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Rabbit Recombinant Monoclonal KMT6 / EZH2 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra) and reacts with Mouse, Human samples.

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Images

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-KMT6 / EZH2 antibody [EPR25353-284] (AB317360), expandable thumbnail
  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-KMT6 / EZH2 antibody [EPR25353-284] (AB317360), expandable thumbnail
  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-KMT6 / EZH2 antibody [EPR25353-284] (AB317360), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)IHC-PICC/IF
Human
Tested
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2 (PubMed:22323599, PubMed:30923826). Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.

Alternative names

KMT6, EZH2, Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6

Recommended products

Rabbit Recombinant Monoclonal KMT6 / EZH2 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra) and reacts with Mouse, Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25353-284
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-KMT6 / EZH2 antibody [EPR25353-284] (ab317360), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-KMT6 / EZH2 antibody [EPR25353-284] (ab317360)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized EZH2 knockout HAP1 (Left) / parental HAP1 (HOWT01) (human chronic myelogenous leukemia near-haploid cell, Right) cells labelling KMT6 / EZH2 with ab317360 at 1/500 dilution(0.1ug)/(Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-KMT6 / EZH2 antibody [EPR25353-284] (ab317360), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-KMT6 / EZH2 antibody [EPR25353-284] (ab317360)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized B16-F10 (mouse skin melanoma cell) cells labelling KMT6 / EZH2 with ab317360 at 1/500 dilution(0.1ug)/(Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-KMT6 / EZH2 antibody [EPR25353-284] (ab317360), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-KMT6 / EZH2 antibody [EPR25353-284] (ab317360)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NCCIT (human pluripotent embryonic carcinoma epithelial cell) cells labelling KMT6 / EZH2 with ab317360 at 1/500 dilution(0.1ug)/(Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com