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AB325782

Alexa Fluor® 647 Anti-LAMA3 antibody [EPR28286-78]

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Rabbit Recombinant Monoclonal LAMA3 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human samples.

View Alternative Names

LAMNA, Laminin subunit alpha-3, Epiligrin 170 kDa subunit, Epiligrin subunit alpha, Kalinin subunit alpha, Laminin-5 subunit alpha, Laminin-6 subunit alpha, Laminin-7 subunit alpha, Nicein subunit alpha, E170

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-LAMA3 antibody [EPR28286-78] (AB325782)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-LAMA3 antibody [EPR28286-78] (AB325782)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling LAMA3 with ab325782 at 1/50 (10.0 µg/ml) dilution.

Positive staining in basement membrane of human colon.

The primary antibody was incubated overnight at 4°C (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-LAMA3 antibody [EPR28286-78] (AB325782)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-LAMA3 antibody [EPR28286-78] (AB325782)

Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling LAMA3 with ab325782 at 1/50 (10.0 µg/ml) dilution

Positive staining in basement membrane of human skin.

The primary antibody was incubated overnight at 4°C (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-LAMA3 antibody [EPR28286-78] (AB325782)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-LAMA3 antibody [EPR28286-78] (AB325782)

Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling LAMA3 with ab325782 at 1/50 (10.0 µg/ml) dilution

Positive staining in basement membrane of human endometrial carcinoma.

The primary antibody was incubated overnight at 4°C (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-LAMA3 antibody [EPR28286-78] (AB325782)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-LAMA3 antibody [EPR28286-78] (AB325782)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling LAMA3 with ab325782 at 1/50 (10.0 µg/ml) dilution

Negative control : no staining in human spleen.

The primary antibody was incubated overnight at 4°C (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28286-78

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin subunit alpha-3 also known by the gene name LAMA3 is a critical component of the extracellular matrix. The protein has a molecular weight of approximately 400 kDa. LAMA3 is primarily expressed in epithelial tissues contributing significantly to the structural integrity of basement membranes. The protein often partners with other laminin subunits to form different laminin isoforms which are essential for cellular adhesion and signaling.
Biological function summary

LAMA3 plays an important role in the formation of stable basement membranes and interacts with other proteins to maintain tissue architecture. As part of the laminin-332 complex alongside beta and gamma laminin chains LAMA3 ensures cell adhesion migration and differentiation processes. Its expression in tissues like skin and mucosal surfaces highlights its function in providing structural support and cellular microenvironment regulation.

Pathways

Laminin subunit alpha-3 is essential in pathways related to cell adhesion and migration such as integrin signaling and epithelial-mesenchymal transition. These pathways are pivotal during wound healing and tissue regeneration. LAMA3 associates with integrins particularly beta-1 and beta-4 integrins facilitating communication between cells and their extracellular environment. The interaction with integrins enables cells to respond to structural changes and external stimuli.

Mutations or alterations in LAMA3 can lead to conditions such as Epidermolysis Bullosa and Junctional Epidermolysis Bullosa where the integrity of the skin and epithelial tissues is compromised. Laminin subunit gamma-2 often interacts with LAMA3 and anomalies in this partnership contribute to these severe blistering disorders. Understanding the link between LAMA3 and these diseases helps inform the development of targeted therapies aimed at restoring normal cell-matrix interactions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.. Laminin-5 is thought to be involved in (1) cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes, (2) signal transduction via tyrosine phosphorylation of pp125-FAK and p80, (3) differentiation of keratinocytes.
See full target information Laminin subunit alpha-3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com