AB223943

Alexa Fluor® 647 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker

RabMAb
Recombinant
What is this?

(1 Review)

(1 Publication)

Rabbit Recombinant Monoclonal Lamin B1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

LMN2, LMNB, LMNB1, Lamin-B1

2 Images

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB223943)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB223943)

Overlay histogram showing HeLa cells stained with ab223943 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223943, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor^® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB223943)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB223943)

ab223943 staining Lamin A + B1 + C in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223943 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
578 PE

PE Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
660 APC

APC Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
HRP

HRP Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
Unconjugated

Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker
Carrier free

Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4068

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody recognizes full length Lamin A/B1/C and the cleaved small unit.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lamin A Lamin B1 and Lamin C are nuclear envelope proteins each representing different forms of lamin molecules. These proteins are important components of the nuclear lamina. Lamin A and Lamin C are encoded by the LMNA gene and result from alternative splicing. Lamin A has a molecular weight of approximately 74 kDa while Lamin C weighs around 65 kDa. Lamin B1 with a molecular weight of about 66 kDa is a product of the LMNB1 gene. These proteins express largely in various tissues and are central to maintaining nuclear structure and integrity.

Biological function summary

Lamin proteins regulate nuclear architecture and maintain chromatin organization. They participate in forming the nuclear lamina a dense fibrillar network inside the nucleus. The lamina provides structural support to the nuclear envelope and plays roles in DNA replication and transcription. Lamin molecules also interact with nuclear pore complexes therefore contributing to molecular transport across the nuclear envelope. Lamin A/C and Lamin B1 function together with other proteins like emerin and lamin associated polypeptides in forming multi-protein complexes that influence cell cycle regulation and differentiation.

Pathways

Lamin proteins integrate into pathways governing the cell cycle and DNA repair. They contribute to the mitotic pathway assisting in the disassembly and reassembly of the nuclear envelope during cell division. Lamin molecules also engage in the DNA damage response pathway cooperating with repair proteins such as p53 and BRCA1. Through these pathways lamin proteins ensure proper progression through the cell cycle and maintain genomic stability by aiding in the repair of DNA damage.

Lamins associate with a variety of nuclear envelopathies and arepsponsible for rare genetic disorders like Hutchinson-Gilford Progeria Syndrome (HGPS) and Emery-Dreifuss Muscular Dystrophy (EDMD). Mutations in the LMNA gene affect nuclear mechanics leading to accelerated aging in HGPS. Similarly disruptions in lamin A/C's interactions with emerin result in EDMD characterized by muscular dystrophy and cardiac issues. These connections highlight the role of lamin proteins in nuclear stability and their impact on disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed : 28716252, PubMed : 32910914). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed : 28716252, PubMed : 32910914). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed : 28716252, PubMed : 32910914).

See full target information LMNB1

Additional targets

LMNA,LMNA

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of tissue engineering 15:20417314241306681 PubMed39712077

2024

Harnessing bone marrow mesenchymal stem cell-derived extracellular vesicles and biomimetic peptide WKYMVm in self-healing hydrogel for enhanced bone repair in femoral defects.

Applications

Unspecified application

Species

Unspecified reactive species

Keyu Luo,Yufei Jin,Baiyi Liu,Yingbo Wang,Yaoyao Liu,Si Qiu,Jianhua Zhao,Xiang Yin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB223943)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB223943)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

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