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AB200427

Alexa Fluor® 647 Anti-Lamin B2 antibody [EPR9701(B)]

4

(1 Review)

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(3 Publications)

Rabbit Recombinant Monoclonal Lamin B2 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

LMN2, LMNB2, Lamin-B2

3 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Lamin B2 antibody [EPR9701(B)] (AB200427)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Lamin B2 antibody [EPR9701(B)] (AB200427)

ab200427 staining Lamin B2 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200427 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2μg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Lamin B2 antibody [EPR9701(B)] (AB200427)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Lamin B2 antibody [EPR9701(B)] (AB200427)

Immunohistochemistry analysis of paraffin-embedded human cerebrum tissue section labelling Lamin B2 with ab200427 at 1/50 dilution. The section was incubated with ab200427 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins.
Nuclear envelope staining on human cerebrum tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Negative control : Phosphate buffered saline (PBS).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Lamin B2 antibody [EPR9701(B)] (AB200427)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Lamin B2 antibody [EPR9701(B)] (AB200427)

Immunohistochemistry analysis of paraffin-embedded human bladder transitional cell carcinoma tissue section labelling Lamin B2 with ab200427 at 1/50 dilution. The section was incubated with ab200427 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins.
Nuclear envelope staining on human bladder transitional cell carcinoma tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Negative control : Phosphate buffered saline (PBS).

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Lamin B2 antibody [EPR9701(B)]

  • 578 PE

    PE Anti-Lamin B2 antibody [EPR9701(B)]

  • 660 APC

    APC Anti-Lamin B2 antibody [EPR9701(B)]

  • HRP

    HRP Anti-Lamin B2 antibody [EPR9701(B)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Lamin B2 antibody [EPR9701(B)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Lamin B2 antibody [EPR9701(B)]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Lamin B2 antibody [EPR9701(B)]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Lamin B2 antibody [EPR9701(B)]

  • Unconjugated

    Anti-Lamin B2 antibody [EPR9701(B)]

  • Carrier free

    Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9701(B)

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lamin B2 also referred to as 'ln43' or 'lmnB2' is a lamin molecule with a molecular weight of approximately 66 kDa. It is an important component of the nuclear lamina an essential structure that provides mechanical support to the nuclear envelope. Lamin B2 is expressed in most cell types and is particularly abundant in cells with high proliferative capacity such as those in the fetal brain and liver. The protein is encoded by the LMNB2 gene and functions as an integral part of the nuclear envelope's architecture by interacting with other nuclear proteins.
Biological function summary

Lamin B2 plays a critical role in maintaining nuclear stability and chromatin organization. It is a part of a larger complex that includes Lamin B and other nuclear matrix proteins. This structural matrix is important for chromosomal segregation during cell division ensuring that the genetic material is evenly distributed between daughter cells. Lamin B2 also facilitates processes such as DNA replication and repair by serving as a scaffold for anchoring various molecular components within the nucleus.

Pathways

Lamin B2 is implicated in the regulation of the mitotic spindle formation during cell division which is a part of the cell cycle pathway. It interacts with proteins such as Lamin A and other nucleoporins contributing to the proper formation of nuclear pore complexes. Furthermore it plays a role in the pathway of apoptosis by modulating the release of factors from the nucleus that trigger cell death mechanisms. These interactions emphasize its importance in maintaining cellular function and integrity.

Alterations in Lamin B2 expression or function have been linked to certain types of cancer such as glioblastoma as well as to laminopathies. Laminopathies are a group of disorders caused by mutations in lamin proteins leading to defects in nuclear structure and function. Besides aberrant Lamin B2 is associated with neurological disorders through its interactions with other nuclear envelope protein components like Lamin A/C. These connections highlight the importance of Lamin B2 in both physiological processes and pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed : 33033404). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed : 33033404). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed : 33033404).
See full target information LMNB2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 5:1263 PubMed36400937

2022

UnMICST: Deep learning with real augmentation for robust segmentation of highly multiplexed images of human tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Clarence Yapp,Edward Novikov,Won-Dong Jang,Tuulia Vallius,Yu-An Chen,Marcelo Cicconet,Zoltan Maliga,Connor A Jacobson,Donglai Wei,Sandro Santagata,Hanspeter Pfister,Peter K Sorger

Cell reports methods 1: PubMed34485971

2021

Toward reproducible, scalable, and robust data analysis across multiplex tissue imaging platforms.

Applications

Unspecified application

Species

Unspecified reactive species

Erik A Burlingame,Jennifer Eng,Guillaume Thibault,Koei Chin,Joe W Gray,Young Hwan Chang

Cancer cell international 20:202 PubMed32514247

2020

Circular RNA circ-CSPP1 regulates CCNE2 to facilitate hepatocellular carcinoma cell growth via sponging miR-577.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Sun,Rui Yu,Chunfeng Wang,Jianning Yao,Lianfeng Zhang
View all publications
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Product promise

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