AB237307

Alexa Fluor® 647 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker

RabMAb
Recombinant
What is this?

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(6 Publications)

Rabbit Recombinant Monoclonal LAMP1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Mouse samples. Cited in 6 publications.

View Alternative Names

CD107a, Lamp-1, Lamp1, Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, 120 kDa lysosomal membrane glycoprotein, CD107 antigen-like family member A, LGP-120, Lysosomal membrane glycoprotein A, P2B, LGP-A

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker (AB237307)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker (AB237307)

ab237307 staining LAMP1 in NIH/3T3 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237307 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker
578 PE

PE Anti-LAMP1 antibody [EPR21026] - Lysosome Marker
Unconjugated

Anti-LAMP1 antibody [EPR21026] - Lysosome Marker
Carrier free

Anti-LAMP1 antibody [EPR21026] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21026

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Mouse

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in NIH/3T3 fixed with 100% methanol (5 min).</p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.

Biological function summary

LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.

Pathways

The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.

LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis (PubMed : 15121881). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (By similarity). Also plays an important role in NK-cells cytotoxicity (PubMed : 23847195). Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (By similarity). In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content (PubMed : 23847195). Presents carbohydrate ligands to selectins (By similarity). Also implicated in tumor cell metastasis (PubMed : 2676155).

See full target information Lamp1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:110504 PubMed40701245

2025

PERK and IRE1α promote exosome secretion via blocking lysosomal degradation of multiple vesicular body.

Applications

Unspecified application

Species

Unspecified reactive species

Shixin Zhou,Zihan Zhou,Si Chen,Ming Wang,Likun Wang

Nature communications 16:1175 PubMed39885146

2025

Oligodendrocyte precursor cells facilitate neuronal lysosome release.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Pao Fang,Ching-Hsin Lin,Yasser Medlej,Renping Zhao,Hsin-Fang Chang,Qilin Guo,Zhonghao Wu,Yixun Su,Na Zhao,Davide Gobbo,Amanda Wyatt,Vanessa Wahl,Frederic Fiore,Szu-Min Tu,Ulrich Boehm,Wenhui Huang,Shan Bian,Amit Agarwal,Marcel A Lauterbach,Chenju Yi,Jianqin Niu,Anja Scheller,Frank Kirchhoff,Xianshu Bai

Journal of leukocyte biology 116:632-643 PubMed38484156

2024

B-1a cells scavenge NETs to attenuate sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Kensuke Murata,Atsushi Murao,Chuyi Tan,Ping Wang,Monowar Aziz

Microbiology spectrum 11:e0225323 PubMed37796020

2023

Lactate promotes intracellular replication and systemic infection via driving macrophage M2 polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyue Wang,Bin Yang,Shuangshuang Ma,Xiaolin Yan,Shuai Ma,Hongmin Sun,Yuyang Sun,Lingyan Jiang

Nature immunology 24:991-1006 PubMed37095377

2023

Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yavuz F Yazicioglu,Eros Marin,Ciaran Sandhu,Silvia Galiani,Iwan G A Raza,Mohammad Ali,Barbara Kronsteiner,Ewoud B Compeer,Moustafa Attar,Susanna J Dunachie,Michael L Dustin,Alexander J Clarke

Scientific reports 13:6393 PubMed37076504

2023

Dentin defects caused by a Dspp frameshift mutation are associated with the activation of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Liang,Charles E Smith,Yuanyuan Hu,Hong Zhang,Chuhua Zhang,Qian Xu,Yongbo Lu,Ling Qi,Jan C-C Hu,James P Simmer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-LAMP1 antibody [EPR21026] - Lysosome Marker (AB237307)

Related conjugates and formulations (6)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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Product promise