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AB225383

Alexa Fluor® 647 Anti-LC3B antibody [EPR18709] - Autophagosome Marker

5

(3 Reviews)

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(10 Publications)

Anti-LC3B antibody [EPR18709] - Autophagosome Marker - Alexa Fluor® 647 conjugated (ab225383) is a rabbit recombinant monoclonal antibody detecting LC3B in ICC/IF. Suitable for Human.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

MAP1ALC3, MAP1LC3B, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1A/MAP1B LC3 B

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-LC3B antibody [EPR18709] - Autophagosome Marker (AB225383)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-LC3B antibody [EPR18709] - Autophagosome Marker (AB225383)

ab225383 staining LC3B in HeLa chloroquine-treated (50μM, 24 hours) cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225383 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-LC3B antibody [EPR18709] - Autophagosome Marker (AB225383)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-LC3B antibody [EPR18709] - Autophagosome Marker (AB225383)

ab225383 staining LC3B in wild-type HAP1 cells and knockout cells, untreated and chloroquine-treated (50μM, 24 hours). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab225383 at 0.5μg/ml (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-LC3B antibody [EPR18709] - Autophagosome Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709]

  • Unconjugated

    Anti-LC3B antibody [EPR18709] - Autophagosome Marker

  • Carrier free

    Anti-LC3B antibody [EPR18709] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18709

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in chloroquine- treated HeLa cells and chloroquine-treated HAP1 cells fixed with 100% methanol (5 min)</p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in chloroquine- treated HeLa cells and chloroquine-treated HAP1 cells fixed with 100% methanol (5 min)</p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-LC3B antibody [EPR18709] - Autophagosome Marker (ab225383) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone EPR18709 is also available pre-conjugated to a variety of labels for your convenience – Anti-LC3B Alexa Fluor® 647 [EPR18709] - Autophagosome Marker (ab225383).

Specificity confirmed
The specificity of Alexa Fluor® 647 Anti-LC3B antibody [EPR18709] - Autophagosome Marker (ab225383) has been confirmed by Immunocytochemistry/ Immunofluorescence testing in MAP1LC3B Knockout HAP1 cell line.

Other related products
We have a range of other formats of antibody clone [EPR18709] also available for your convenience: ab192890, Carrier free - ab221794, Alexa Fluor® 488 - ab225382, Alexa Fluor® 647 - ab225383, Alexa Fluor® 555 - ab307768, ab322912

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LC3B also known as microtubule-associated protein 1 light chain 3 beta plays an important role in autophagy a process that maintains cellular homeostasis. The LC3B protein with a molecular weight of approximately 14 kDa undergoes lipidation to form LC3-II which associates with autophagosomal membranes. This protein expresses ubiquitously in various tissues including liver muscle and brain. Researchers often detect LC3B using techniques like LC3B western blot and LC3B immunofluorescence due to its function as a marker indicating autophagy levels.
Biological function summary

LC3B contributes significantly to the formation and maturation of autophagosomes. LC3B part of the autophagy-related protein complex binds to autophagic membranes. During this process LC3-I converts to LC3-II a lipid-phosphatidylethanolamine conjugate essential for autophagosome membrane expansion and closure. This mechanism helps remove damaged organelles and misfolded proteins from cells therefore contributing to cellular quality control.

Pathways

LC3B integrates into the autophagy pathway which is critical for cellular adaptive responses to stress. The mammalian target of rapamycin (mTOR) pathway regulates autophagy where mTOR inhibition activates LC3B promoting autophagosome formation. Moreover LC3B operates alongside other proteins like Beclin-1 and ULK1 facilitating the initiation and progression of autophagy under nutrient starvation conditions. These interactions highlight LC3's role in cellular energy balance and survival mechanisms.

LC3B connects with conditions such as cancer and neurodegenerative diseases. Altered autophagy levels mediated by LC3B often associate with tumorigenesis where its dysregulation can affect cancer progression. Furthermore LC3B also links to neurodegenerative diseases like Alzheimer's where impaired autophagy disrupts neuronal function. LC3B interacts with proteins such as p62/SQSTM1 which affects protein aggregate clearance a critical factor in neurodegenerative pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed : 20418806, PubMed : 23209295, PubMed : 28017329). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed : 23209295, PubMed : 28017329). In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria (PubMed : 22922758). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed : 20418806, PubMed : 23209295, PubMed : 28017329). Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (PubMed : 24089205). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed : 31006537, PubMed : 31006538). Upon nutrient stress, directly recruits cofactor JMY to the phagophore membrane surfaces and promotes JMY's actin nucleation activity and autophagosome biogenesis during autophagy (PubMed : 30420355).
See full target information MAP1LC3B
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadv8640 PubMed40512859

2025

Interaction between NF-κB and PLAC8 impairs autophagy providing a survival advantage to prostate cells transformed by cadmium.

Applications

Unspecified application

Species

Unspecified reactive species

Vaibhav Shukla,Ashish Tyagi,Balaji Chandrasekaran,Bhawna Tyagi,Balpreet Singh,Thulasidharan Nair Devanarayanan,Venkatesh Kolluru,Murali K Ankem,Chendil Damodaran

Scientific reports 15:15077 PubMed40301490

2025

The potentially therapeutic effects of ascorbic acid in different cell line in attempt to reduce the risk of radiation therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Rasha S Shams El Dine,Heba T Youseef,Ashraf K Awaad,Sabahh I Hammoury,Ehab I Mohamed

Journal for immunotherapy of cancer 12: PubMed38926151

2024

Tumor cell-released autophagosomes (TRAPs) induce PD-L1-decorated NETs that suppress T-cell function to promote breast cancer pulmonary metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohe Zhou,Chengdong Wu,Xuru Wang,Ning Pan,Xiaotong Sun,Bohao Chen,Shiya Zheng,Yiting Wei,Jing Chen,Yuyang Wu,Fengjiao Zhu,Jinpeng Chen,Huabiao Chen,Li-Xin Wang

Journal of immunology research 2022:1260423 PubMed35615533

2022

Overexpression of TP53INP2 Promotes Apoptosis in Clear Cell Renal Cell Cancer via Caspase-8/TRAF6 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xunjia Li,Daixing Hu,Ying Li,Yan Luo,Bing Liang,Kexiao Yu,Weijian Xiong,Deyu Zuo

Food science & nutrition 10:2773-2785 PubMed35959262

2022

Vitamin D ameliorates asthma-induced lung injury by regulating HIF-1α/Notch1 signaling during autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Chaowen Huang,Ming Peng,Jinzhai Tong,Xueying Zhong,Jun Xian,Liandi Zhong,Jiongrui Deng,Yanming Huang

PLoS pathogens 18:e1010159 PubMed34995322

2022

Rhinovirus C replication is associated with the endoplasmic reticulum and triggers cytopathic effects in an in vitro model of human airway epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Talita B Gagliardi,Monty E Goldstein,Daniel Song,Kelsey M Gray,Jae W Jung,Maxinne A Ignacio,Kimberly M Stroka,Gregg A Duncan,Margaret A Scull

Oncology letters 22:695 PubMed34457050

2021

miR-425 regulates lipophagy via SIRT1 to promote sorafenib resistance in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Gongping Sun,Liang Yang,Shibo Wei,Hongyuan Jin,Bowen Li,Hangyu Li

Journal of receptor and signal transduction resear :1-8 PubMed33043765

2020

Isoflurane activates AMP-activated protein kinase to inhibit proliferation, and promote apoptosis and autophagy in cervical carcinoma both and .

Applications

Unspecified application

Species

Unspecified reactive species

Hongfang Wei,Tianze Sun,Jie Liu,Xiaowei Wang,Guangping Zhao,Jiong Shi,Yongxue Chen

RSC advances 10:1498-1506 PubMed35494689

2020

Retracted Article: LncRNA SNHG5 regulates the cell viability and apoptosis of glioma cells by the miR-1297/KPNA2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xueyuan Li,Qiankun Liu,Kang Wang,Wenzheng Luo,Tiansong Liang,Shanpeng Yuan,Yingwei Zhen,Dongming Yan

Nature communications 10:3668 PubMed31413316

2019

Autophagy inhibition elicits emergence from metastatic dormancy by inducing and stabilizing Pfkfb3 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Alyssa La Belle Flynn,Benjamin C Calhoun,Arishya Sharma,Jenny C Chang,Alexandru Almasan,William P Schiemann
View all publications
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