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AB322021

Alexa Fluor® 647 Anti-Lhx2/LH2 antibody [EPR20449]

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Rabbit Recombinant Monoclonal Lhx2/LH2 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Mouse, Rat samples.

View Alternative Names

LH2, LHX2, LIM/homeobox protein Lhx2, Homeobox protein LH-2, LIM homeobox protein 2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Lhx2/LH2 antibody [EPR20449] (AB322021)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Lhx2/LH2 antibody [EPR20449] (AB322021)

Immunohistochemical analysis of paraffin-embedded Rat telencephalon tissue labeling Lhx2/LH2 with ab322021 at 1/50 (10.0 ug/ml) dilution.

Nuclear staining on rat telencephalon.
The section was incubated with ab322021 for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Lhx2/LH2 antibody [EPR20449] (AB322021)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Lhx2/LH2 antibody [EPR20449] (AB322021)

Immunohistochemical analysis of paraffin-embedded Mouse telencephalon tissue labeling Lhx2/LH2 with ab322021 at 1/50 (10.0 ug/ml) dilution.

Nuclear staining on mouse telencephalon.
The section was incubated with ab322021 for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20449

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Lhx2 protein also known as LH2 is a member of the LIM homeobox family of transcription factors. It has a molecular mass of approximately 45 kDa. Known for its dual LIM domains and a homeodomain Lhx2 directly binds DNA and regulates gene expression. This protein is expressed in various tissues including the developing brain retina and liver. Notably Lhx2 plays a role in embryonic development by impacting the growth and differentiation of several tissue types.
Biological function summary

Researchers link the Lhx2 protein to key processes in cellular differentiation and organ development. Lhx2 often partners within transcriptional complexes that guide cellular fate decisions. By interacting with other cofactors Lhx2 influences the expression of genes involved in tissue-specific differentiation and morphogenesis. This regulatory action is essential for the proper formation and function of certain organs and structures in the body.

Pathways

Lhx2 impacts several intricate biological pathways notably in neural development. It functions within the Wnt signaling pathway where it modulates neural progenitor cell populations in the central nervous system. Through this pathway Lhx2 associates with proteins like β-catenin to control gene expression linked to brain development. Additionally Lhx2 is involved in the Sonic hedgehog (Shh) signaling pathway aligning with proteins such as Gli1 to influence neuronal patterning.

Mutations or dysregulation of Lhx2 have connections to conditions like anophthalmia and brain malformations. Aberrant expression of Lhx2 can disrupt normal development contributing to these congenital anomalies. Furthermore Lhx2's interaction with proteins including Pax6 plays a role in ocular development and anomalies in these interactions can lead to abnormal eye formation. Researchers continue to investigate the significance of Lhx2 in various developmental diseases to develop targeted therapeutic approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types (By similarity).
See full target information LHX2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com