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Rabbit Recombinant Monoclonal Ly6g antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P, Flow Cyt and reacts with Mouse samples. Cited in 1 publication.

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Images

Flow Cytometry - Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135] (AB303467), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135] (AB303467), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135] (AB303467), expandable thumbnail

Publications

  • BMC medical genomics 16:3142023
    Key mA regulators mediated methylation modification pattern and immune infiltration characterization in hepatic ischemia-reperfusion injury.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Yixi Zhang,Can Qi,Yiwen Guo,Xuefeng Li,Zebin Zhu
    PubMed 38049811

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow Cyt
Mouse
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Version

    Target data

    Function

    Alternative names

    Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g

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    Rabbit Recombinant Monoclonal Ly6g antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P, Flow Cyt and reacts with Mouse samples. Cited in 1 publication.

    Alternative names

    Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g

    Key facts

    Isotype

    IgG

    Conjugation

    Alexa Fluor® 647

    Excitation/Emission

    Ex: 650nm, Em: 665nm

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Clone number

    EPR22909-135

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate short-term storage duration

    1-2 weeks

    Appropriate short-term storage conditions

    +4°C

    Appropriate long-term storage conditions

    -20°C

    Aliquoting information

    Upon delivery aliquot

    Storage information

    Avoid freeze / thaw cycle, Store in the dark

    Notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Supplementary info

    Activity summary

    Ly6G also known as lymphocyte antigen 6 complex locus G6D 1A8 or 8C5 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein. The molecule has a molecular weight of about 25-30 kDa. Its expression is mainly on neutrophils and a subpopulation of eosinophils in mice. It plays a role in the innate immune system marking these immune cells for differentiation and response to infection. Researchers consider it a specific marker for detecting and isolating mouse neutrophils often using methods such as Ly6G staining or anti-Ly6G antibodies for identification.

    Biological function summary

    Ly6G functions in the immune response by facilitating the migration and adhesion of neutrophils to the site of infection or injury. It is part of a protein complex on the cell surface that includes other Ly6 proteins. This group of proteins helps to modulate signal transduction for immune cell functions. Ly6G serves as an anchor through its GPI linkage aiding cell-to-cell communication and signal amplification in inflammation processes.

    Pathways

    Ly6G is significantly integrated into the immune signaling pathways notably the toll-like receptor (TLR) signaling pathway. Through these pathways Ly6G influences neutrophil migration and activation. Closely associated proteins include CD11b and CD18 which interact with Ly6G in neutrophil functions including adhesion and transmigration. These pathways are essential for orchestrating the body's defense mechanisms against pathogens.

    Associated diseases and disorders

    Ly6G plays an essential role in inflammatory conditions and autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of Ly6G can alter neutrophil behavior which contributes to disease pathology. Researchers are investigating the relationship between Ly6G and other immune-related proteins like myeloperoxidase (MPO) and elastase as these protein interactions may impact the development and progression of inflammatory diseases.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
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    3 product images

    • Flow Cytometry - Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135] (ab303467), expandable thumbnail

      Flow Cytometry - Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135] (ab303467)

      Flow Cytometry analysis of mouse blood cells (Right panel) compared to Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) (Left panel) using ab303467 at a 1/50 dilution (1µg).
      Cells were stained with rabbit IgG or ab303467. Then stained with anti-CD11b conjugated to BV510.
      Gated on viable cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135] (ab303467), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135] (ab303467)

      Immunohistochemical analysis of paraffin-embedded mouse lung tissue stained for Ly6g using ab303467 at a 1/50 dilution (10.0 μg/ml) (Red). Positive staining on scattered cells (arrows) in mouse lung.
      The section was incubated with ab303467 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
      The immunostaining was performed on a Leica Biosystems BOND RX instrument.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135] (ab303467), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135] (ab303467)

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue stained for Ly6g using ab303467 at a 1/50 dilution (10.0 μg/ml) (Red). Positive staining on scattered neutrophil-like cells (arrows) in mouse spleen.
      The section was incubated with ab303467 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
      The immunostaining was performed on a Leica Biosystems BOND RX instrument.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins).

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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