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AB320783

Alexa Fluor® 647 Anti-LY75/DEC-205 antibody [EPR27222-93]

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Rabbit Recombinant Monoclonal LY75 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt and reacts with Mouse samples.

View Alternative Names

CD205, Cd205, SMU_610, spaP, Lymphocyte antigen 75, Ly-75, DEC-205

1 Images
Flow Cytometry - Alexa Fluor® 647 Anti-LY75/DEC-205 antibody [EPR27222-93] (AB320783)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Anti-LY75/DEC-205 antibody [EPR27222-93] (AB320783)

Flow cytometric analysis of Mouse splenocyte cells labelling LY75/DEC-205 with ab320783 at 1/500 dilution (0.1ug) (Red) and Blue compared with a isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Cells were stained with CD11c conjugated to PE/CY7 and CD8a conjugated to Brilliant Violet 421. Gated on viable CD11c+ CD8a neg and CD11c+ CD8a+ populations.

  • 578 PE

    PE Anti-LY75/DEC-205 antibody [EPR27222-93]

  • Unconjugated

    Anti-LY75/DEC-205 antibody [EPR27222-93]

  • Carrier free

    Anti-LY75/DEC-205 antibody [EPR27222-93] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27222-93

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Not suitable for Flow Cytometry in human samples.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein LY75 also known as DEC-205 is a type I transmembrane glycoprotein belonging to the C-type lectin receptor family. It has a molecular mass of approximately 205 kDa. LY75 is expressed mainly on dendritic cells macrophages and B lymphocytes playing a critical role in antigen uptake and processing. The receptor exhibits high-affinity binding to carbohydrate structures facilitating its function in the immune response.
Biological function summary

LY75/DEC-205 functions primarily in the immune system by mediating endocytosis of antigens for processing and presentation to T cells. It operates either alone or as part of a larger protein complex guiding immune surveillance and response. In particular it influences the maturation and activation of dendritic cells critical processes for initiating adaptive immunity.

Pathways

LY75/DEC-205 is integral to the antigen processing and presentation pathway interacting significantly with proteins like the major histocompatibility complex (MHC) molecules. The receptor also engages in the Toll-like receptor signaling pathway linking innate and adaptive immunity through its collaborations with these receptor proteins.

LY75/DEC-205 associates with autoimmune diseases and certain cancers. Its expression and function in dendritic cells implicate it in autoimmune conditions such as lupus where immune regulation is disrupted. It may also relate to tumor progression in cancers with an immune evasion component like melanoma. This connection involves proteins like PD-L1 which tumor cells express to escape immune recognition.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment. Causes reduced proliferation of B lymphocytes (By similarity).
See full target information Ly75
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com