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Rabbit Recombinant Monoclonal MAdCAM1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, IHC-P and reacts with Transfected cell line - Human, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAdCAM1 antibody [EPR27555-87] (AB317666), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAdCAM1 antibody [EPR27555-87] (AB317666), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAdCAM1 antibody [EPR27555-87] (AB317666), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MAdCAM1 antibody [EPR27555-87] (AB317666), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-P
Human
Expected
Tested
Transfected cell line - Human
Tested
Not recommended

Tested
Tested

Species

Transfected cell line - Human

Dilution info

1/250

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Transfected cell line - Human

Dilution info

-

Notes

-

Target data

Function

Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal MAdCAM1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, IHC-P and reacts with Transfected cell line - Human, Human samples.

Key facts

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR27555-87

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MAdCAM-1 also known as mucosal addressin cell adhesion molecule 1 or mucosal tag is a protein with a molecular mass of approximately 60 kDa. The MAdCAM-1 protein is expressed prominently on high endothelial venules within mucosal tissues such as the gut and the colon. Additionally it also appears in the endothelial cells of lymphoid tissues. Another name for it is MECA-79 highlighting its recognition by the MECA-79 antibody. Mucosal tags label regions where MAdCAM-1 assists in cell adhesion processes.

Biological function summary

MAdCAM-1 facilitates lymphocyte homing to mucosal tissues. It plays a part in the adhesive interactions between endothelial cells and lymphocytes as a component of the immune surveillance system enabling T cells and B cells to move into areas where they are needed. MAdCAM-1 forms complexes with integrins notably alpha4beta7 which are integral to its adhesion functions. Through its role in guiding lymphocytes it influences immune response and inflammation in mucosal environments.

Pathways

MAdCAM-1 contributes importantly to the trafficking of immune cells. It is involved in the regulation of cell adhesion and migration pathways. The protein interacts extensively with the integrin family especially integrin alpha4beta7 which facilitates lymphocyte entry into the intestinal wall. Additionally it works with chemokine receptors and cytokines that further influence cell movement and homing to specific tissues. This integrative role reinforces its importance in immune response and mucosal immunity.

Associated diseases and disorders

MAdCAM-1 links closely to inflammatory diseases such as Crohn's disease and ulcerative colitis. Its expression is upregulated in these inflammatory conditions which leads to increased lymphocyte accumulation in the gut. This condition exacerbates the inflammation and tissue damage characteristic of these disorders. MECA-79 a monoclonal antibody targets MAdCAM-1 to aid therapeutic approaches in mitigating inflammation driven by lymphocyte infiltration. Understanding MAdCAM-1 and its function offers potential pathways to target and treat these chronic inflammatory diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAdCAM1 antibody [EPR27555-87] (ab317666), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAdCAM1 antibody [EPR27555-87] (ab317666)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling MAdCAM1 with ab317666 at 1/50 (10.0 ug/ml) dilution.

    Negative control: no staining on human skeletal muscle. The section was incubated with ab317666 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAdCAM1 antibody [EPR27555-87] (ab317666), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAdCAM1 antibody [EPR27555-87] (ab317666)

    Immunohistochemical analysis of paraffin-embedded Human gastric adenoc tissue labeling MAdCAM1 with ab317666 at 1/50 (10.0 ug/ml) dilution.

    Positive staining on blood vessels of human gastric adenocarcinoma. The section was incubated with ab317666 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAdCAM1 antibody [EPR27555-87] (ab317666), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAdCAM1 antibody [EPR27555-87] (ab317666)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MAdCAM1 with ab317666 at 1/50 (10.0 ug/ml) dilution.

    Positive staining on blood vessels of human colon. The section was incubated with ab317666 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MAdCAM1 antibody [EPR27555-87] (ab317666), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MAdCAM1 antibody [EPR27555-87] (ab317666)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling MAdCAM1 with ab317666 at 1/250 (2.0 ug/ml) dilution (Green).

    Confocal image showing positive staining in 293T cells transfected with a human MAdCAM1 expression vector containing a myc tag (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 488 Anti-Myc tag antibody [9E10] ab202008 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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