Alexa Fluor® 647 Anti-MAdCAM1 antibody [EPR27555-87]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MAdCAM1 antibody [EPR27555-87] (AB317666)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling MAdCAM1 with ab317666 at 1/250 (2.0 ug/ml) dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a human MAdCAM1 expression vector containing a myc tag (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab202008 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAdCAM1 antibody [EPR27555-87] (AB317666)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling MAdCAM1 with ab317666 at 1/50 (10.0 ug/ml) dilution.

Negative control : no staining on human skeletal muscle. The section was incubated with ab317666 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAdCAM1 antibody [EPR27555-87] (AB317666)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MAdCAM1 with ab317666 at 1/50 (10.0 ug/ml) dilution.

Positive staining on blood vessels of human colon. The section was incubated with ab317666 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAdCAM1 antibody [EPR27555-87] (AB317666)

Immunohistochemical analysis of paraffin-embedded Human gastric adenoc tissue labeling MAdCAM1 with ab317666 at 1/50 (10.0 ug/ml) dilution.

Positive staining on blood vessels of human gastric adenocarcinoma. The section was incubated with ab317666 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.