AB225315

Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] - Neuronal Marker

Name: Alexa Fluor® 647 conjugated Anti-MAP2 antibody [EPR19691] - Neuronal Marker (ab225315) | Abcam
Brand: Abcam Limited
SKU: AB225315
Price: 565 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(3 Publications)

Anti-MAP2 antibody [EPR19691] - Alexa Fluor® 647 conjugated (ab225315) is a rabbit recombinant monoclonal antibody detecting MAP2 in IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Microtubule-associated protein 2, MAP-2, MAP2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB225315)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB225315)

IHC image of MAP2 staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6) in a Biocare Medical NxGen pressure cooker using retrieval settings of 110^oC for 20 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab225315 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB225315)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum tissue labelling with ab225315 at 1 : 50 dilution (red). The nuclear counterstain was DAPI (Blue).

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB225315)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB225315)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labelling with ab225315 at 1 : 50 dilution (red). The nuclear counterstain was DAPI (Blue).

Carrier free

Anti-MAP2 antibody [EPR19691] – Chicken IgY (Chimeric) – BSA and Azide Free
Carrier free

Anti-MAP2 antibody [EPR19691] - BSA and Azide free
Unconjugated

Anti-MAP2 antibody [EPR19691] - Neuronal Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-MAP2 antibody [EPR19691] - Neuronal Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-MAP2 antibody [EPR19691] - Neuronal Marker
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] - Neuronal Marker
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-MAP2 antibody [EPR19691] - Neuronal Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19691

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), IHC-P, IHC-Fr, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Suitable for mouse and rat IHC-Fr

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "IHC-Fr is suitable for mouse and rat", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "IHC-Fr is suitable for mouse and rat", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] (ab225315) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Mouse, Rat samples.

Related products
Antibody clone EPR19691 is also available pre-conjugated to a variety of labels for your convenience – Anti-MAP2 Alexa Fluor® 647 [EPR19691] (ab225315).

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

See full target information MAP2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113334 PubMed40917877

2025

Human cerebral organoids model tumor initiation and infiltration in an autologous astrocyte-supported setting.

Applications

Unspecified application

Species

Unspecified reactive species

Esther Schickel,Tamara Bender,Leon Kaysan,Simone Hufgard,Margot Mayer,David R Grosshans,Christiane Thielemann,Insa S Schroeder

Scientific reports 15:3215 PubMed39863746

2025

The role of Box A of HMGB1 in producing γH2AX associated DNA breaks in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sirapat Settayanon,Pithi Chanvorachote,Apiwat Mutirangura

Communications medicine 3:170 PubMed38017287

2023

AAV-mediated editing of PMP22 rescues Charcot-Marie-Tooth disease type 1A features in patient-derived iPS Schwann cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Yoshioka,Juliana Bosso Taniguchi,Hidenori Homma,Takuya Tamura,Kyota Fujita,Maiko Inotsume,Kazuhiko Tagawa,Kazuharu Misawa,Naomichi Matsumoto,Masanori Nakagawa,Haruhisa Inoue,Hikari Tanaka,Hitoshi Okazawa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] - Neuronal Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB225315)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB225315)

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB225315)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise