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Anti-MAP2 [EPR19691] Alexa Fluor® 647 conjugated antibody (ab225315) rabbit monoclonal antibody that is used to detect MAP2 in IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB225315), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB225315), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB225315), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-FrIHC-P
Human
Expected
Tested
Mouse
Tested
Expected
Rat
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

IHC-Fr is suitable for mouse and rat

Species
Rat
Dilution info
1/50
Notes

IHC-Fr is suitable for mouse and rat

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
1/100
Notes

-

Species
Mouse
Dilution info
1/100
Notes

-

Associated Products

Select an associated product type

13 products for Alternative Product

5 products for Alternative Version

Target data

Function

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

Alternative names

Microtubule-associated protein 2, MAP-2, MAP2

Recommended products

Anti-MAP2 [EPR19691] Alexa Fluor® 647 conjugated antibody (ab225315) rabbit monoclonal antibody that is used to detect MAP2 in IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19691
Purification technique
Affinity purification Protein A
Specificity

Suitable for mouse and rat IHC-Fr

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

What is this antibody validated in?


Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] (ab225315) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), in Human, Mouse, Rat samples.

Recommended positive controls


IHC-P: Normal human cerebral cortex tissue sections.IHC-Fr: Mouse and Rat cerebellum tissue sections.

Related products


Antibody clone EPR19691 is also available pre-conjugated to a variety of labels for your convenience – Anti-MAP2 Alexa Fluor® 647 [EPR19691] (ab225315).

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (ab225315), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (ab225315)

    MAP2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-MAP2 antibody

    IHC image of MAP2 staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6) in a Biocare Medical NxGen pressure cooker using retrieval settings of 110oC for 20 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab225315 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (ab225315), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (ab225315)

    MAP2 Immunohistochemistry (Frozen sections) staining of Mouse cerebellum using rabbit Anti-MAP2 antibody

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labelling with ab225315 at 1:50 dilution (red). The nuclear counterstain was DAPI (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (ab225315), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MAP2 antibody [EPR19691] - Neuronal Marker (ab225315)

    MAP2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat cerebellum using rabbit Anti-MAP2 antibody

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum tissue labelling with ab225315 at 1:50 dilution (red). The nuclear counterstain was DAPI (Blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com