JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB207386

Alexa Fluor® 647 Anti-MAT2A + MAT1A antibody [EPR10496]

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal MAT1A antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

AMS1, MATA1, MAT1A, S-adenosylmethionine synthase isoform type-1, AdoMet synthase 1, Methionine adenosyltransferase 1, Methionine adenosyltransferase I/III, MAT 1, MAT-I/III

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MAT2A + MAT1A antibody [EPR10496] (AB207386)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MAT2A + MAT1A antibody [EPR10496] (AB207386)

ab207386 staining MAT2A in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207386 at a 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10496

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAT2A (Methionine Adenosyltransferase 2A) and MAT1A (Methionine Adenosyltransferase 1A) are enzymes that catalyze the formation of S-adenosylmethionine (SAM) from methionine and ATP. MAT2A also known as hepatic MAT has a mass of approximately 52 kDa while MAT1A also sometimes referred to as extrahepatic MAT typically weighs around 48 kDa. Both enzymes differ in their tissue expression: MAT1A is predominantly found in the liver whereas MAT2A expresses across various tissues such as the brain and intestines.
Biological function summary

MAT2A and MAT1A are key in the methionine cycle impacting gene regulation through methylation reactions. They facilitate the synthesis of SAM a universal methyl donor essential for transmethylation processes within cells. Specifically MAT1A forms a complex with glycine N-methyltransferase to regulate homocysteine and methylation homeostasis. Both enzymes perform a role in controlling the abundance of SAM which influences various cellular processes including nucleic acid protein and lipid metabolism.

Pathways

MAT2A and MAT1A participate actively in methionine and SAM metabolism. They integrate within the one-carbon metabolism pathway which is critical for methylation processes affecting gene expression and epigenetic regulation. MAT2A interacts with proteins like MAT2B which modulates the activity of MAT2A impacting the balance between SAM and S-adenosylhomocysteine. This pathway interaction is important for maintaining methylation balance and cellular proliferation.

MAT2A and MAT1A relate to liver diseases and cancer reflecting their roles in SAM metabolism. Abnormal function or expression of these enzymes affects SAM levels linking to liver cirrhosis and hepatocellular carcinoma. MAT1A mutations can impair methionine metabolism exacerbating liver conditions. Similarly altered MAT2A expression associates with certain cancers where it modulates cellular methylation states and growth. Proteins like SAM might get affected creating an imbalance that contributes to the pathology of liver disease and oncogenesis.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme : formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.
See full target information MAT1A

Additional targets

MAT2A
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2022:4036274 PubMed35069973

2022

Oxidative Stress Aggravates Apoptosis of Nucleus Pulposus Cells through mA Modification of MAT2A Pre-mRNA by METTL16.

Applications

Unspecified application

Species

Unspecified reactive species

Peng-Bo Chen,Gui-Xun Shi,Tao Liu,Bo Li,Sheng-Dan Jiang,Xin-Feng Zheng,Lei-Sheng Jiang

Cell systems 11:478-494.e9 PubMed33113355

2020

Receptor-Driven ERK Pulses Reconfigure MAPK Signaling and Enable Persistence of Drug-Adapted BRAF-Mutant Melanoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Luca Gerosa,Christopher Chidley,Fabian Fröhlich,Gabriela Sanchez,Sang Kyun Lim,Jeremy Muhlich,Jia-Yun Chen,Sreeram Vallabhaneni,Gregory J Baker,Denis Schapiro,Mariya I Atanasova,Lily A Chylek,Tujin Shi,Lian Yi,Carrie D Nicora,Allison Claas,Thomas S C Ng,Rainer H Kohler,Douglas A Lauffenburger,Ralph Weissleder,Miles A Miller,Wei-Jun Qian,H Steven Wiley,Peter K Sorger
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB186129

Anti-MAT2A antibody - N-terminal

Western blot - Anti-MAT2A antibody - N-terminal (AB186129)

  • 0
  • 0
Primary Antibodies

AB154343

Anti-MAT2A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAT2A antibody (AB154343)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB252420

Anti-METTL16 antibody [EPR23459-39]

RabMAb|Recombinant

Immunoprecipitation - Anti-METTL16 antibody [EPR23459-39] (AB252420)

  • 0
  • 0
Primary Antibodies

AB13847

Anti-Caspase-3 antibody

KO Validated

Western blot - Anti-Caspase-3 antibody (AB13847)

  • 4
  • 56
Primary Antibodies

AB129176

Anti-MAT1A + MAT2A antibody [EPR7938]

RabMAb|Recombinant

Immunoprecipitation - Anti-MAT1A + MAT2A antibody [EPR7938] (AB129176)

  • 0
  • 0
Primary Antibodies

AB177477

Anti-IMP3 antibody [EPR12021]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-IMP3 antibody [EPR12021] (AB177477)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com