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AB210425

Alexa Fluor® 647 Anti-MCM5 antibody [EP2683Y]

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(1 Publication)

Rabbit Recombinant Monoclonal MCM5 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

CDC46, MCM5, DNA replication licensing factor MCM5, CDC46 homolog, P1-CDC46

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MCM5 antibody [EP2683Y] (AB210425)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MCM5 antibody [EP2683Y] (AB210425)

ab210425 staining MCM5 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210425 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2683Y

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Human, Mouse

Applications

ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB6154

Anti-MCM5 antibody [CRCT5.1 (A2.7A3)]

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Primary Antibodies

AB17967

Anti-MCM5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody (AB17967)

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Primary Antibodies

AB76023

Anti-MCM5 antibody [EP2682Y]

RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (AB76023)

  • 1
  • 1

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

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Primary Antibodies

AB199091

Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199091)

  • 0
  • 0
Primary Antibodies

AB199093

Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein MCM5 also known as Minichromosome Maintenance Complex Component 5 plays a significant role in DNA replication. It is a part of the minichromosome maintenance (MCM) family which consists of several proteins forming an important component of the pre-replication complex. MCM5 has a molecular mass of approximately 82 kDa. This protein is ubiquitously expressed in cells underlining its fundamental role in the cell cycle. The presence of MCM5 ensures the fidelity and timing of DNA replication initiation during the S phase of cell division.
Biological function summary

As a component of the MCM2-7 hexameric complex MCM5 contributes to the unwinding of DNA an essential step for replication. This complex acts as a helicase unwinding the DNA double helix at replication origins. This activity is required for laying down the replication fork and allowing access for the DNA polymerase. MCM5 integrates into the larger assembly of the replication machinery working in concert with cell cycle regulators to ensure proper cell cycle progression.

Pathways

MCM5 participates in DNA replication and cell cycle control pathways. It closely associates with the GINS complex and CDC45 forming the CMG (CDC45-MCM-GINS) complex which is critical for replication fork progression. MCM5 also interacts with the ATR-mediated DNA damage repair pathway. This relationship provides a checkpoint mechanism to halt replication in response to DNA damage allowing time for repair processes to maintain genomic stability.

MCM5 has implications in cancer and replication stress-related conditions. Overexpression or dysregulation of MCM5 can lead to uncontrolled cell proliferation contributing to oncogenesis. MCM5 has a notable connection to cyclin D1 and the Retinoblastoma (Rb) protein pathway often disrupted in cancers. Moreover in diseases characterized by replication stress such as certain neurodegenerative disorders improper MCM5 function can exacerbate genomic instability leading to disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells (PubMed : 40940420). Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 16899510, PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 32453425).
See full target information MCM5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Regenerative therapy 26:203-212 PubMed38948130

2024

Modified human skin cell isolation protocol and its influence on keratinocyte and melanocyte culture.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Liu,Shunxin Jin,Dapeng Cheng,Hao Chen,Yuxiang Wang,Chao Ji,Zhenzhen Yan,Xiao Fang,Shichu Xiao,Xinling Bi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com