JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB204638

Alexa Fluor® 647 Anti-MEKK2 antibody [EP626Y]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal MEKK2 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

MAPKKK2, MEKK2, MAP3K2, Mitogen-activated protein kinase kinase kinase 2, MAPK/ERK kinase kinase 2, MEK kinase 2, MEKK 2

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MEKK2 antibody [EP626Y] (AB204638)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MEKK2 antibody [EP626Y] (AB204638)

ab204638 staining MEKK2 in MCF7 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204638 at 1/500 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Carrier free

    Anti-MEKK2 antibody [EP626Y] - BSA and Azide free

  • Unconjugated

    Anti-MEKK2 antibody [EP626Y]

  • 578 PE

    PE Anti-MEKK2 antibody [EP626Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP626Y

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

Flow Cyt (Intra), ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Proteins & Peptides

AB85299

Recombinant human MEKK2 protein

Functional Studies - Recombinant human MEKK2 protein (AB85299)

  • 0
  • 0
Primary Antibodies

AB178876

Anti-MEK1 + MEK2 antibody [EPR16667]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 + MEK2 antibody [EPR16667] (AB178876)

  • 5
  • 1
Primary Antibodies

AB184699

Anti-ERK1 + ERK2 antibody [EPR17526]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-ERK1 + ERK2 antibody [EPR17526] (AB184699)

  • 5
  • 1
Primary Antibodies

AB76299

Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunoprecipitation - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (AB76299)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p>This product gave a positive signal in MCF7 cells fixed with 100% methanol (5 min).</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEKK2 also known as MAP3K2 is a kinase enzyme with an approximate mass of 70 kDa. It functions as part of the MAP kinase signaling pathway where it phosphorylates downstream proteins playing an essential role in transmitting signals within cells. This protein is mainly expressed in tissues such as the brain heart and liver. It acts by activating specific MAP kinase pathways facilitating various cellular responses to external stimuli.
Biological function summary

This kinase serves as a critical mediator in cellular processes like proliferation differentiation and apoptosis. MEKK2 forms part of larger signaling complexes including the MAP3K complex where it regulates multiple cellular activities. Through its kinase activity MEKK2 passes on signals that affect gene expression and cellular stress responses impacting how cells react to their environment.

Pathways

MEKK2 functions prominently in the MAPK signaling and NF-kB pathways. These pathways involve signal transduction mechanisms that MEKK2 influences through its interactions with related proteins such as MEK5 and ERK5. By modulating these pathways MEKK2 participates in a variety of cellular responses including inflammation and immune responses.

MEKK2 has associations with conditions like cancer and inflammatory diseases. Aberrant expression of MEKK2 can affect the behavior of cancer cells due to its influence on the MAPK pathway's signaling leading to unchecked cell growth. Additionally MEKK2 interacts with proteins like NF-kB which are important in inflammation thereby implicating it in disorders related to immune system dysfunctions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and-run dynamics.
See full target information MAP3K2
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neurotoxicity research 31:545-559 PubMed28155214

2017

RA Differentiation Enhances Dopaminergic Features, Changes Redox Parameters, and Increases Dopamine Transporter Dependency in 6-Hydroxydopamine-Induced Neurotoxicity in SH-SY5Y Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fernanda M Lopes,Leonardo Lisbôa da Motta,Marco A De Bastiani,Bianca Pfaffenseller,Bianca W Aguiar,Luiz F de Souza,Geancarlo Zanatta,Daiani M Vargas,Patrícia Schönhofen,Giovana F Londero,Liana M de Medeiros,Valder N Freire,Alcir L Dafre,Mauro A A Castro,Richard B Parsons,Fabio Klamt
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com