AB225500

Alexa Fluor® 647 Anti-MelanA antibody [EPR20380]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal MelanA antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 5 publications.

View Alternative Names

MART1, MLANA, Melanoma antigen recognized by T-cells 1, MART-1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A

2 Images

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-MelanA antibody [EPR20380] (AB225500)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-MelanA antibody [EPR20380] (AB225500)

Overlay histogram showing Malme-3M cells stained with ab225500 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225500, 1/50000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor^® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

This antibody gave a positive signal in Malme-3M cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MelanA antibody [EPR20380] (AB225500)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MelanA antibody [EPR20380] (AB225500)

ab225500 staining MelanA in Malme-3M cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225500 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

578 PE

PE Anti-MelanA antibody [EPR20380]
660 APC

APC Anti-MelanA antibody [EPR20380]
Unconjugated

Anti-MelanA antibody [EPR20380]
Carrier free

Anti-MelanA antibody [EPR20380] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20380

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human, Human, Mouse

Applications

Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "This product gave a positive signal in Malme-3M cells fixed with 100% methanol (5 min)", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50000", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.

See full target information MLANA

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in medicine 12:1583271 PubMed40421296

2025

Injectable crosslinked HA hydrogel: a promising carrier for cell transplantation to treat stable vitiligo.

Applications

Unspecified application

Species

Unspecified reactive species

Qianren Zheng,Jie Chen,Yixun Huang,Weikai Chen,Dandan Cheng,Qianqian Jia,Meiqin Zhu,Youguo Liao,Qiulin He,Shunli Wu

Cancer immunology research 12:1421-1437 PubMed38949179

2024

NK Receptor Signaling Lowers TCR Activation Threshold, Enhancing Selective Recognition of Cancer Cells by TAA-Specific CTLs.

Applications

Unspecified application

Species

Unspecified reactive species

Bowen Dong,Nataša Obermajer,Takemasa Tsuji,Junko Matsuzaki,Cindy M Bonura,Cindy Sander,Henry Withers,Mark D Long,Colin Chavel,Scott H Olejniczak,Hans Minderman,John M Kirkwood,Robert P Edwards,Walter J Storkus,Pedro Romero,Pawel Kalinski

Regenerative therapy 26:203-212 PubMed38948130

2024

Modified human skin cell isolation protocol and its influence on keratinocyte and melanocyte culture.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Liu,Shunxin Jin,Dapeng Cheng,Hao Chen,Yuxiang Wang,Chao Ji,Zhenzhen Yan,Xiao Fang,Shichu Xiao,Xinling Bi

Nano research 15:2300-2314 PubMed36089987

2022

CD137 agonist potentiates the abscopal efficacy of nanoparticle-based photothermal therapy for melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Preethi Bala Balakrishnan,Debbie K Ledezma,Juliana Cano-Mejia,Jaclyn Andricovich,Erica Palmer,Vishal A Patel,Patricia S Latham,Eric S Yvon,Alejandro Villagra,Rohan Fernandes,Elizabeth E Sweeney

Frontiers in cell and developmental biology 8:191 PubMed32363189

2020

CD38 Causes Autophagic Flux Inhibition and Cardiac Dysfunction Through a Transcriptional Inhibition Pathway Under Hypoxia/Ischemia Conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyue Zhang,Lingfei Li,Qiong Zhang,Qinglin Wei,Jiezhi Lin,Jiezhi Jia,Junhui Zhang,Tiantian Yan,Yanling Lv,Xupin Jiang,Peng Zhang,Huapei Song,Dongxia Zhang,Yuesheng Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-MelanA antibody [EPR20380]

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-MelanA antibody [EPR20380] (AB225500)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MelanA antibody [EPR20380] (AB225500)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Product promise