AB252135

Alexa Fluor® 647 Anti-Mesothelin antibody [EPR19025-42]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal Mesothelin antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

MPF, MSLN, Mesothelin, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

1 Images

Flow Cytometry - Alexa Fluor® 647 Anti-Mesothelin antibody [EPR19025-42] (AB252135)

Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 647 Anti-Mesothelin antibody [EPR19025-42] (AB252135)

Flow cytometry overlay histogram showing HeLa cells stained with ab252135 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum followed by the antibody (ab252135) (1x 10⁶ in 100μl at 1 μg/ml (1/500)) for 30 min on ice.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor^® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 660/20 bandpass filter.

578 PE

PE Anti-Mesothelin antibody [EPR19025-42]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Mesothelin antibody [EPR19025-42]
Unconjugated

Anti-Mesothelin antibody [EPR19025-42]
Carrier free

Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free (Capture)
Carrier free

Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19025-42

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human, Human

Applications

Flow Cyt, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Membrane-anchored forms may play a role in cellular adhesion.. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

See full target information MSLN

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1563095 PubMed40792273

2025

Insights into the role of MSLN-positive circulating tumor cell as an auxiliary diagnostic biomarker in epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Xu,Min Wang,Shuting Wu,Qinke Li,Jinlong Wang,Siying Zhang,Qiongming Liu,Mengting Wang,Ruifang Li,Zhiyuan Hu,Yi Liu,Zhu Yang

British journal of pharmacology 182:5057-5070 PubMed40258390

2025

FGF1 ameliorates DSS-induced ulcerative colitis by reducing neutrophil recruitment through the MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Feng,Yanyan Jin,Xinrui Ni,Haoxin Zheng,Linling Wu,Ying Xia,Changzhi Zhou,Tong Liang,Yunfei Zhu,Juyi Xu,Qijin Wu,Yong Yang,Longwei Zhao,Shentian Zhuang,Xianjing Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-Mesothelin antibody [EPR19025-42]

Flow Cytometry - Alexa Fluor® 647 Anti-Mesothelin antibody [EPR19025-42] (AB252135)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise