JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB307520

Alexa Fluor® 647 Anti-MIP-1 alpha/CCL3 + CCL3L1 antibody [EPR22529-19]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal MIP-1 alpha/CCL3 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.

View Alternative Names

G0S19-1, MIP1A, SCYA3, CCL3, C-C motif chemokine 3, G0/G1 switch regulatory protein 19-1, Macrophage inflammatory protein 1-alpha, PAT 464.1, SIS-beta, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein, MIP-1-alpha

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MIP-1 alpha/CCL3 + CCL3L1 antibody [EPR22529-19] (AB307520)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MIP-1 alpha/CCL3 + CCL3L1 antibody [EPR22529-19] (AB307520)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling MIP-1 alpha/CCL3 + CCL3L1 with ab307520 at 1/250 (2.0 ug/ml) dilution. Confocal image showing cytoplasmic staining in THP-1 treated with Phorbol-12-myristate-13-acetate (100ng/ml) for 56 h, followed by adding Lipopolysaccharide (1ug/ml) for a further 16 h. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-MIP-1 alpha/CCL3 + CCL3L1 antibody [EPR22529-19]

  • 578 PE

    PE Anti-MIP-1 alpha/CCL3 + CCL3L1 antibody [EPR22529-19]

  • Unconjugated

    Anti-MIP-1 alpha/CCL3 + CCL3L1 antibody [EPR22529-19]

  • Carrier free

    Anti-MIP-1 alpha/CCL3 + CCL3L1 antibody [EPR22529-19] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22529-19

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MIP-1 alpha also known as CCL3 or CCL3L1 is a chemokine with important roles in immune regulation. It has a molecular mass of approximately 7.8 kDa. This protein is expressed mainly in immune cells like macrophages T cells and natural killer (NK) cells. The expression of MIP-1 alpha occurs in response to inflammatory signals and it acts to recruit other immune cells to the site of infection or injury. Its function is essential for orchestrating the cellular movements during immune surveillance and defensive responses.
Biological function summary

The MIP-1 alpha functions as a chemotactic cytokine. It binds to receptors like CCR1 and CCR5 on the surface of target cells resulting in activation and migration toward areas of inflammation. MIP-1 alpha does not form a complex with other proteins; however its interactions with its receptors are vital for signal transduction. It also promotes the production of additional inflammatory cytokines contributing to amplifying immune responses. The chemotactic properties of MIP-1 alpha make it an important player in the body's defense against pathogens.

Pathways

MIP-1 alpha is involved in important immune pathways like the NF-kB signaling pathway. This pathway plays a significant role in immune cell activation and the inflammatory response. MIP-1 alpha interacts with other chemokines like CCL4 (MIP-1 beta) which shares similar functions and binds to CCR5. The coordinated activities of these chemokines enhance the recruitment and activation of various leukocyte populations facilitating an effective immune response.

MIP-1 alpha exhibits a connection to inflammatory diseases such as rheumatoid arthritis and HIV infection. In rheumatoid arthritis MIP-1 alpha contributes to joint inflammation and damage by attracting immune cells to synovial joints. In the context of HIV it interacts with the CCR5 receptor affecting viral entry into CD4+ T cells. The inhibition of MIP-1 alpha and its interaction with CCR5 provides a therapeutic strategy in controlling HIV progression. These associations highlight the chemokine's impact on both autoimmune disorders and infectious diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
See full target information CCL3

Additional targets

CCL3L1
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB229400

Human MIP-1 alpha/CCL3 ELISA Kit, Fluorescent

CatchPoint SimpleStep ELISA|Recombinant

Sandwich ELISA - Human MIP-1 alpha/CCL3 ELISA Kit, Fluorescent (AB229400)

  • 0
  • 0
Primary Antibodies

AB45690

Anti-CCL4/MIP-1 beta antibody [EP521Y]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-CCL4/MIP-1 beta antibody [EP521Y] (AB45690)

  • 1
  • 1
Primary Antibodies

AB259372

Anti-CCL3 / MIP-1 alpha + CCL3L1 antibody [EPR23751-54]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL3 / MIP-1 alpha + CCL3L1 antibody [EPR23751-54] (AB259372)

  • 0
  • 0
Primary Antibodies

AB303458

Alexa Fluor® 647 Anti-IL-6 antibody [EPR21711]

RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-IL-6 antibody [EPR21711] (AB303458)

  • 0
  • 0
Primary Antibodies

AB183218

Anti-TNF alpha antibody [EPR19147]

KO Validated|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [EPR19147] (AB183218)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com