Alexa Fluor® 647 Mouse IgG2a monoclonal [PPV-04] - Isotype Control
IgG2a
Mouse
Alexa Fluor® 647
Ex: 650nm, Em: 665nm
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: PBS
Liquid
Monoclonal
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application Flow Cyt | Reactivity Expected | Dilution info - | Notes The reagent is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg per ml of incubation mixture), change the conditions of your experiment to reduce the background. |
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Alexa Fluor® 647 Mouse IgG2a monoclonal [PPV-04] - Isotype Control
IgG2a
Mouse
Alexa Fluor® 647
Ex: 650nm, Em: 665nm
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: PBS
Liquid
Monoclonal
PPV-04
Size-exclusion chromatography
Purified from TCS. The purified antibody is conjugated with Alexa Fluor® 647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Blue Ice
+4°C
+4°C
Store in the dark
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