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Rabbit Recombinant Monoclonal MSH3 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MSH3 antibody [EPR4334(2)] (AB225125), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/100
Notes

This product gave a positive signal in HT-29 cells fixed with 4% formaldehyde (10 min)

Associated Products

Select an associated product type

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Target data

Function

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.

Alternative names

DUC1, DUG, MSH3, DNA mismatch repair protein Msh3, hMSH3, Divergent upstream protein, Mismatch repair protein 1, DUP, MRP1

Recommended products

Rabbit Recombinant Monoclonal MSH3 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4334(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The mutS homolog 3 (MSH3) protein plays an important role in DNA mismatch repair. It is part of the family of MutS proteins and is sometimes referred to by its gene name MSH3. MSH3 forms a heterodimer with MSH2 to create MutSβ. This complex targets insertion and deletion loops. The mass of MSH3 is approximately 127 kDa. Expression of MSH3 occurs in various tissues with high levels in the colon small intestine and testis.

Biological function summary

MSH3 contributes to genome stability by correcting DNA replication errors. As part of the MutSβ complex it identifies and initiates repair of insertion and deletion loops which helps maintain genomic integrity. In eukaryotes MSH3 along with its partner MSH2 cooperates with other proteins such as MLH1 and PMS2 to ensure proper mismatch repair. These interactions help to prevent mutations from becoming permanent.

Pathways

MSH3 participates in the DNA mismatch repair pathway important for maintaining genomic fidelity. It works closely with MSH2 to form MutSβ enhancing the repair of small loops. Another important association includes the MLH1-PMS2 complex within the mismatch repair pathways. This collaboration ensures errors in DNA during replication do not lead to genomic instability.

Associated diseases and disorders

Mutations or malfunctioning of MSH3 have been associated with colorectal cancer and Lynch syndrome. Alterations in MSH3 can impair DNA repair processes contributing to cancer development. The MSH2 protein which pairs with MSH3 also plays a critical role in these disorders. Understanding MSH3 function helps illuminate its involvement in genetic stability and its impact on health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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1 product image

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MSH3 antibody [EPR4334(2)] (ab225125), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MSH3 antibody [EPR4334(2)] (ab225125)

    ab225125 staining MSH3 in HT-29 (human colorectal adenocarcinoma cell line) cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225125 at 1/100 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com