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AB320369

Alexa Fluor® 647 Anti-Munc 13-4 antibody [EPR4914]

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Rabbit Recombinant Monoclonal Munc 13-4 antibody - conjugated to Alexa Fluor® 647. Suitable for Antibody Labelling, Target Binding Affinity, Flow Cyt (Intra) and reacts with Human, Mouse samples.

View Alternative Names

Protein unc-13 homolog D, Munc13-4, UNC13D

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Munc 13-4 antibody [EPR4914]

  • 578 PE

    PE Anti-Munc 13-4 antibody [EPR4914]

  • 660 APC

    APC Anti-Munc 13-4 antibody [EPR4914]

  • Unconjugated

    Anti-Munc 13-4 antibody [EPR4914]

  • Carrier free

    Anti-Munc 13-4 antibody [EPR4914] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4914

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Mouse

Applications

Antibody Labelling, Target Binding Affinity, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "AntibodyLabelling" : {"fullname" : "Antibody Labelling", "shortname":"Antibody Labelling"}, "TargetBindingAffinity" : {"fullname" : "Target Binding Affinity", "shortname":"Target Binding Affinity"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Munc13-4 also known as UNC13D acts as a critical mediator in the fusion of vesicles particularly in the exocytosis of cytotoxic granules in immune cells. This protein weighs approximately 120 kDa and resides in various immune cell types such as cytotoxic T lymphocytes and natural killer cells. Munc13-4 exists in the cytosol and relocates to the cell membrane upon activation supporting its role in exocytosis.
Biological function summary

Munc13-4 serves an essential function in immune response by facilitating granule-mediated cytotoxicity. This protein does not operate alone; it acts within a complex network with other members of the Unc13 protein family. The synergy between these proteins ensures the precise docking and priming of secretory vesicles aiding in effective immune responses against infected or malignant cells.

Pathways

Munc13-4 participates in the granule exocytosis pathway a central component of immune system functionality. Within this pathway Munc13-4 acts alongside proteins such as Syntaxin 11 and Rab27a influencing the regulation and release of cytolytic granules. Another vital pathway involving Munc13-4 is the calcium-dependent signaling pathway which modulates its activity and highlights its exocytotic role.

Defects in Munc13-4 have a strong association with familial hemophagocytic lymphohistiocytosis type 3 (FHL3) a severe immunological disorder. The impaired function of Munc13-4 results in defective cytotoxic granule exocytosis leading to improper immune regulation. Additionally connections exist between Munc13-4 dysfunction and systemic lupus erythematosus (SLE) which implicates proteins such as Perforin and Rab27a in altered immune responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Regulates assembly of recycling and late endosomal structures, leading to the formation of an endosomal exocytic compartment that fuses with perforin-containing granules at the immunologic synapse and licences them for exocytosis. Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells.
See full target information UNC13D
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com