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Anti-Myc tag [9E10] Alexa Fluor® 647 conjugated antibody (ab223895) mouse monoclonal antibody that is used to detect Myc tag in Flow Cytometry (Intra), Flow Cytometry, ICC/IF.


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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (AB223895), expandable thumbnail
  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (AB223895), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (AB223895), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (AB223895), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (AB223895), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)ICC/IF
Tag
Tested
Tested

Tested
Tested

Species
Tag
Dilution info
1/500
Notes

-

Tested
Tested

Species
Tag
Dilution info
1/100
Notes

This product gave a positive signal in 293T cells transfected with TMEM119 with myc tag fixed with 4% formaldehyde (10 min).

Associated Products

Select an associated product type

14 products for Alternative Version

11 products for Alternative Product

Target data

Function

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed:24940000, PubMed:25956029). Activates the transcription of growth-related genes (PubMed:24940000, PubMed:25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000, PubMed:25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808).

Alternative names

BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39

Recommended products

Anti-Myc tag [9E10] Alexa Fluor® 647 conjugated antibody (ab223895) mouse monoclonal antibody that is used to detect Myc tag in Flow Cytometry (Intra), Flow Cytometry, ICC/IF.


Key facts

Isotype
IgG1
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
9E10
Purity
IgG fraction
Specificity

This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc. A publication by Baker AM et al. 2016 (PMID: 26826706 DOI: 10.1111/his.12939) shows the IHC staining generated by the 9E10 clone does not correlate with c-myc mRNA expression.

Epitope
aa 410-419 of human Myc.
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

What is this antibody validated in?


Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF).

Tagged protein detection


Myc tag antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Related products


Antibody clone 9E10 is also available pre-conjugated to a variety of labels for your convenience – Anti-Myc tag Alexa Fluor® 647 [9E10] (ab223895).

Other related products


We have a range of other formats of antibody clone [9E10] also available for your convenience: Anti-Myc tag antibody [9E10] ab32, Carrier free - DyLight® 550 Anti-Myc tag antibody [9E10] ab117511, Biotin - Biotin Anti-Myc tag antibody [9E10] - C-terminal ab197139, Anti-Myc tag antibody [9E10] ab206486, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] ab223894, Alexa Fluor® 647 - ab223895, APC - APC Anti-Myc tag antibody [9E10] ab223896



Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

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9 product images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney) transfected with a Nucleoprotein expression vector containing a myc-tag. cells labelling HCoV-OC43 Nucleoprotein with Anti-HCoV-OC43 Nucleoprotein antibody [abd384] ab309964 at 1/100 (10.87 ug/ml) dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) antibody at 1/100 dilution (Green).
    Confocal image showing cytoplasmic staining in 293T cells transfected with HCoV-OC43 Nucleoprotein expression vector containing a myc tag. No staining on HEK-293T transfected with empty vector containing a myc-tag.
    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
    ab223895 Anti-Myc tag Mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) at 1/100 dilution.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)

    Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-tagged TMEM119 expression vector labelling Myc-tag with ab223895 at 1/500 dilution (right) compared with Mouse IgG1 (monoclonal) Alexa Fluor® 647 (left). Cells were surface-stained with TMEM119 antibody (Anti-TMEM119 antibody [106-6] - Microglial marker ab210405), then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag antibody (ab223895) and Alexa Fluor® 488 conjugated anti-Rabbit secondary antibody (for TMEM119). Only TMEM119 (+) population showed Myc-tag positive staining.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)

    ab223895 staining Myc-tag (pseudocolored in white) in 293T cells transfected with TMEM119 with myc tag. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223895 at 1/100 dilution and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue) and Alexa Fluor® 488 conjugated control Myc-tag antibody (different clone) is shown in green.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Flow Cytometry - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)

    Flow Cytometry analysis of HEK-293T (Human embryonic kidney epithelial cell) cells transfected with a SARS-CoV-2 nsp9 (Left) or SARS-CoV2 nsp15 (Right) expression vector containing a myc tag labeling SARS-CoV2 nsp15 with Anti-SARS-CoV-2 nsp15 antibody [EPR24840-74] ab284629 at 1/500 dilution (left and right). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1/5000 dilution. Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895) was used to label transfected cells.

  • Flow Cytometry - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)

    Flow cytometric analysis 4% Paraformaldehyde fixed 90% methanol permeabilised HEK-293T (Human embryonic kidney epithelial cell) cells transfected with a SARS-CoV-2 nsp1 (Left) or SARS-CoV2 nsp13 (Right) expression vector containing a myc tag labelling SARS-CoV-2 nsp13 with Anti-SARS-CoV-2 nsp13 antibody [EPR24838-18] ab286909 at 1/50 dilution. Goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895) was used to label transfected cells.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney) transfected with a Nucleoprotein expression vector containing a myc-tag. cells labelling HCoV-OC43 Nucleoprotein with Anti-HCoV-OC43 Nucleoprotein antibody [abd385] ab309966 at 1/90 (10.36 ug/ml) dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) antibody at 1/100 dilution (Green).
    Confocal image showing cytoplasmic staining in 239T cells transfected with HCoV-OC43 Nucleoprotein expression vector containing a myc tag. No staining on HEK-293T transfected with empty vector containing a myc-tag.
    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
    ab223895 Anti-Myc tag Mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) at 1/100 dilution.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling hMPV prefusion F with Anti-hMPV fusion Protein antibody [EPR29365-579] ab320652 at 1/1000 (0.517 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing positive staining in 293T cells transfected with a hMVP prefusion F expression vector containing a myc-His-tag®(shown in green) but no staining in 293T cells transfected with a hRSV prefusion F expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    ab223895 Anti-Myc tag Mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK293T (human embryonic kidney epithelial cell) cells labelling Stra8 with Anti-Stra8 antibody [EPR27083-56] ab308124 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

    Confocal image showing nuclear staining in HEK-293T cells transfected with a mouse STRA8 expression vector containing a myc tag.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab223895 Anti-Myc tag Mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 dilution (10 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney) transfected with a CBX1-V5-tagged expression vector containing a His-tag. cells labelling V5 tag with Anti-V5 tag antibody [RM1083] ab314555 at 1/500 (1.08 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1015 (2ug/ml) dilution (Green). Confocal image showing nuclear staining in 293T cells transfected with V5 tag protein expression vector containing a myc tag, no staining on HEK-293T transfected with empty vector containing a His-tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab223895 Anti-Myc tag Mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain at 1/100 (5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1015 (2ug/ml) dilution.

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