JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB315963

Alexa Fluor® 647 Anti-Myosin heavy chain antibody [A4.1025]

Be the first to review this product! Submit a review

|

(0 Publication)

Anti-Myosin heavy chain antibody [A4.1025] (ab315963) is a mouse monoclonal antibody - conjugated to Alexa Fluor® 647 - detecting myosin heavy chains 1,2,3,4,5,6,7 and 8 in IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat.

View Alternative Names

Myosin-1, Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d, MyHC-2x, MyHC-IIx/d, MYH1, MYHSA2, MYH2, Myosin-2, Myosin heavy chain 2, Myosin heavy chain 2a, Myosin heavy chain IIa, MyHC-2a, MyHC-IIa

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Myosin heavy chain antibody [A4.1025] (AB315963)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Myosin heavy chain antibody [A4.1025] (AB315963)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Myosin (MYH 1/2) with ab315963 at 1/100 (5.0 ug/ml) dilution.

Negative control : no staining on human liver. The section was incubated with ab315963 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Myosin heavy chain antibody [A4.1025] (AB315963)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Myosin heavy chain antibody [A4.1025] (AB315963)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Myosin (MYH 1/2) with ab315963 at 1/100 (5.0 ug/ml) dilution.

Cytoplasmic staining on human cardiac muscle. The section was incubated with ab315963 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Myosin heavy chain antibody [A4.1025] (AB315963)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Myosin heavy chain antibody [A4.1025] (AB315963)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Myosin (MYH 1/2) with ab315963 at 1/100 (5.0 ug/ml) dilution.

Cytoplasmic staining on human skeletal muscle. The section was incubated with ab315963 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Myosin heavy chain antibody [A4.1025] (AB315963)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Myosin heavy chain antibody [A4.1025] (AB315963)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Myosin (MYH 1/2) with ab315963 at 1/500 (1.0 ug/ml) dilution (Green).

Negative control : confocal image showing no staining on rat liver (PMID : 30274168). The nuclear counterstain was DAPI (Blue). The section was incubated with ab315963 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Myosin heavy chain antibody [A4.1025] (AB315963)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Myosin heavy chain antibody [A4.1025] (AB315963)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Myosin (MYH 1/2) with ab315963 at 1/100 (5.0 ug/ml) dilution.

Cytoplasmic staining on rat skeletal muscle. The section was incubated with ab315963 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Myosin heavy chain antibody [A4.1025] (AB315963)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Myosin heavy chain antibody [A4.1025] (AB315963)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Myosin (MYH 1/2) with ab315963 at 1/100 (5.0 ug/ml) dilution.

Cytoplasmic staining on mouse skeletal muscle. The section was incubated with ab315963 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Myosin heavy chain antibody [A4.1025] (AB315963)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Myosin heavy chain antibody [A4.1025] (AB315963)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Rat skeletal muscle (fresh) tissue labeling Myosin (MYH 1/2) with ab315963 at 1/500 (1.0 ug/ml) dilution (Green).

Confocal image showing positive staining on rat skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab315963 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Myosin heavy chain antibody [A4.1025] (AB315963)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Myosin heavy chain antibody [A4.1025] (AB315963)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling Myosin (MYH 1/2) with ab315963 at 1/500 (1.0 ug/ml) dilution (Green).

Negative control : confocal image showing no staining on mouse liver (PMID : 30274168). The nuclear counterstain was DAPI (Blue). The section was incubated with ab315963 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Myosin heavy chain antibody [A4.1025] (AB315963)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Myosin heavy chain antibody [A4.1025] (AB315963)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (fresh) tissue labeling Myosin (MYH 1/2) with ab315963 at 1/500 (1.0 ug/ml) dilution (Green).

Confocal image showing positive staining on mouse skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab315963 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Carrier free

    Anti-Myosin heavy chain antibody [A4.1025] - BSA and Azide free

  • Unconjugated

    Anti-Myosin heavy chain antibody [A4.1025]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Myosin heavy chain antibody [A4.1025]

  • HRP

    HRP Anti-Myosin antibody [A4.1025]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

A4.1025

Isotype

IgG2a

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Mouse, Rat, Human, Mouse, Rat

Applications

IHC-Fr, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ATP binding loop of myosin heavy chain skeletal and cardiac muscle.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myosin is a molecular motor protein responsible for converting chemical energy into mechanical force facilitating muscle contraction and cellular movement. Also known as the myosin heavy chain (MHC) or MYH myosin has a commonly referenced heavy chain Myosin heavy chain 1 (MYH1) with a molecular mass of roughly 200 kDa. Myosin is broadly expressed in muscle tissues including skeletal cardiac and smooth muscles and appears in various isoforms depending on the specific type of muscle tissue.
Biological function summary

Myosin interacts intricately with actin filaments to form a complex that executes muscle contraction and cell motility. Myosin heads bind to actin filaments employing ATP hydrolysis to generate motion. This protein is a fundamental component of the sarcomere the structural unit of muscle fibers and works in concert with other proteins like tropomyosin and troponin to regulate muscle contraction.

Pathways

Myosin has a significant role in the muscle contraction and cell motility pathways. Myosin and actin interaction is central to the sliding filament theory which is an essential part of the muscle contraction pathway. Myosin is also involved in non-muscle cell movement through the actin-myosin contractile ring in the process of cytokinesis. Myosin interacts with proteins such as actin and ATP in these pathways to facilitate cellular processes.

Myosin has connections to conditions like hypertrophic cardiomyopathy and various myopathies. Mutations in the myosin heavy chain can lead to hypertrophic cardiomyopathy affecting the heart's ability to pump blood effectively. Additionally disruptions in myosin function can contribute to muscular diseases impairing muscle strength and function. Myosin's interaction with actin and related sarcomeric proteins is pivotal in these pathological scenarios often resulting in altered muscle dynamics and associated clinical manifestations.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Required for normal hearing. It plays a role in cochlear amplification of auditory stimuli, likely through the positive regulation of prestin (SLC26A5) activity and outer hair cell (OHC) electromotility.
See full target information MYH1

Additional targets

MYH2
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com