AB195186

Alexa Fluor® 647 Anti-N Cadherin antibody [EPR1791-4]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal N Cadherin antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

CD325, CDHN, NCAD, CDH2, Cadherin-2, CDw325, Neural cadherin, N-cadherin

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-N Cadherin antibody [EPR1791-4] (AB195186)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-N Cadherin antibody [EPR1791-4] (AB195186)

IHC image of N Cadherin staining in a section of formalin-fixed paraffin-embedded normal human liver.

Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab195186 at 1/100 dilution 5.0 μg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval using ab93678 (Citrate buffer, pH 6.0).

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-N Cadherin antibody [EPR1791-4]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-N Cadherin antibody [EPR1791-4]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-N Cadherin antibody [EPR1791-4]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-N Cadherin antibody [EPR1791-4]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-N Cadherin antibody [EPR1791-4]
Unconjugated

Anti-N Cadherin antibody [EPR1791-4]
Carrier free

Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free
Carrier free

Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1791-4

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-Cadherin also known as CDH2 or neuronal cadherin is a calcium-dependent cell adhesion protein. It is characterized by its role in mediating intercellular connections primarily through its presence on neurons fibroblasts and certain epithelial cells. N-Cadherin has an approximate mass of 130 kDa. The protein is integral in forming homophilic interactions where N-Cadherin molecules on adjacent cells interact to establish robust cell-cell adhesion.

Biological function summary

N-Cadherin significantly influences cell-cell interaction and communication facilitating cellular adhesion and signal transduction. It is a vital component of adherens junctions contributing to tissue morphogenesis and stability. N-Cadherin interacts with other cytoplasmic proteins such as catenins forming a complex essential for linking the actin cytoskeleton to the cell membrane. This interaction affects cellular behaviors including migration and differentiation.

Pathways

N-Cadherin plays a significant role in the neural development and epithelial-to-mesenchymal transition (EMT) pathways important for development and cancer progression. It engages with related proteins such as beta-catenin which helps transduce signals within these pathways. N-Cadherin's interactions within these pathways highlight its role in maintaining multicellular structure and signaling processes important for development and pathogenesis.

N-Cadherin is associated with cancer and heart disease. In cancer particularly in the context of the EMT N-Cadherin facilitates tumor progression and metastasis cooperating with proteins like Twist and Snail to promote these processes. In heart disease alterations in N-Cadherin expression and function can impact cardiac muscle integrity and syncytium highlighting potential interactions with connexin43 to maintain cardiac function. Understanding these associations provides insights into therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone : upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).

See full target information CDH2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 10:572895 PubMed33117705

2020

Detection of EGFR Mutations in cfDNA and CTCs, and Comparison to Tumor Tissue in Non-Small-Cell-Lung-Cancer (NSCLC) Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan E Liu,Meghah Vuppalapaty,Charles Wilkerson,Corinne Renier,Michael Chiu,Clementine Lemaire,James Che,Melissa Matsumoto,James Carroll,Steve Crouse,Violet R Hanft,Stefanie S Jeffrey,Dino Di Carlo,Edward B Garon,Jonathan Goldman,Elodie Sollier

Analytical chemistry 91:11078-11084 PubMed31373191

2019

Single-Cell Phenotypic Profiling of CTCs in Whole Blood Using an Integrated Microfluidic Device.

Applications

Unspecified application

Species

Unspecified reactive species

Haimeng Pei,Lu Li,Yuan Wang,Renjie Sheng,Yiguo Wang,Shuting Xie,Lingling Shui,Haibin Si,Bo Tang

Journal of experimental & clinical cancer research 37:237 PubMed30249278

2018

Long noncoding RNA MLK7-AS1 promotes ovarian cancer cells progression by modulating miR-375/YAP1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Yan,Hong Li,Pengyun Li,Xia Li,Jianjian Lin,Linlin Zhu,Maria A Silva,Xiaofang Wang,Ping Wang,Zhan Zhang

NPJ genomic medicine 2:34 PubMed29263843

2017

Workflow optimization of whole genome amplification and targeted panel sequencing for CTC mutation detection.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan E Liu,Melanie Triboulet,Amin Zia,Meghah Vuppalapaty,Evelyn Kidess-Sigal,John Coller,Vanita S Natu,Vida Shokoohi,James Che,Corinne Renier,Natalie H Chan,Violet R Hanft,Stefanie S Jeffrey,Elodie Sollier-Christen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-N Cadherin antibody [EPR1791-4]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-N Cadherin antibody [EPR1791-4] (AB195186)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise