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AB310068

Alexa Fluor® 647 Anti-Ndufs4 antibody [EP7832]

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Rabbit Recombinant Monoclonal Ndufs4 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.

View Alternative Names

Complex I-18 kDa, Complex I-AQDQ, NADH-ubiquinone oxidoreductase 18 kDa subunit, CI-18 kDa, CI-AQDQ, NDUFS4

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Ndufs4 antibody [EP7832] (AB310068)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Ndufs4 antibody [EP7832] (AB310068)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Ndufs4 with ab310068 at 1/50 (10.0 ug/ml) dilution. ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution. Confocal image showing cytoplasmic staining in HeLa cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Ndufs4 antibody [EP7832] (AB310068)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Ndufs4 antibody [EP7832] (AB310068)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling Ndufs4 with ab310068 at 1/50 (10.0 ug/ml) dilution. ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution. Confocal image showing cytoplasmic staining in HepG2 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 578 PE

    PE Anti-Ndufs4 antibody [EP7832]

  • 660 APC

    APC Anti-Ndufs4 antibody [EP7832]

  • HRP

    HRP Anti-Ndufs4 antibody [EP7832]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Ndufs4 antibody [EP7832]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Ndufs4 antibody [EP7832]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Ndufs4 antibody [EP7832]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Ndufs4 antibody [EP7832]

  • Unconjugated

    Anti-Ndufs4 antibody [EP7832]

  • Carrier free

    Anti-Ndufs4 antibody [EP7832] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP7832

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }
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Product details

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ndufs4 also known as NADH:Ubiquinone Oxidoreductase Subunit S4 is an important component of mitochondrial complex I which is part of the electron transport chain. It has a molecular mass of approximately 18 kDa. The protein is mainly found in the inner mitochondrial membrane where it plays an essential role in cellular respiration by transferring electrons from NADH to ubiquinone. Ndufs4 is widely expressed in tissues with high energy demands such as the brain and heart.
Biological function summary

Ndufs4 functions as a part of mitochondrial complex I a large protein enzyme complex critical for oxidative phosphorylation. The complex comprises multiple subunits of which Ndufs4 is a small but essential part. By participating in electron transfer and proton pumping Ndufs4 supports ATP synthesis which is the main energy currency of the cell. Deficiency in Ndufs4 leads to dysfunctional energy production linked with metabolic disorders.

Pathways

The protein is an integral part of the respiratory chain pathway where it supports oxidative phosphorylation enabling effective ATP production. Within these pathways Ndufs4 interacts with other complex I subunits including Ndufs1 and Ndufa9 which stabilize electron transport. Disruptions in this chain can affect cellular energy metabolism and have downstream effects on various cellular processes.

Mutations in the Ndufs4 gene are associated with Leigh syndrome and mitochondrial complex I deficiency. Leigh syndrome is a severe neurological disorder characterized by progressive loss of mental and movement abilities. Ndufs4 mutations also lead to complex I deficiency causing diverse symptoms ranging from neurological impairments to muscle weakness. These disorders highlight the critical link between Ndufs4 and proper mitochondrial function. The protein's connection to other complex I subunits such as Ndufs1 emphasizes its importance in maintaining regular cellular functions and preventing mitochondrial diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
See full target information NDUFS4
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