Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker

41 product images

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat dorsal root ganglia (fresh) tissue labeling Nav1.8/SCN10A with Anti-Nav1.8/SCN10A antibody [EPR25132-222] ab307817 at 1/50 dilution (10.64 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).
  Panel A: merged staining of anti-Nav1.8/SCN10A (Anti-Nav1.8/SCN10A antibody [EPR25132-222] ab307817, green) and anti-NeuN (ab190565, red) on rat dorsal root ganglia.
  Panel B: anti-Nav1.8/SCN10A stained on rat dorsal root ganglia.
  Panel C: anti-NeuN stained in neurons of rat dorsal root ganglia.
  The section was incubated in two rounds of staining: in the order of Anti-Nav1.8/SCN10A antibody [EPR25132-222] ab307817 and ab190565 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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  He WM et al ci Rep. 2019 Feb 19;9(1):2287. doi: 10.1038/s41598-019-38750-0. Fig 5a. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  Effects of delayed treatment of L655,708 on neurogenesis in the peri-infarct region. Ki67, a proliferative marker, and NeuN, a neuronal marker, were used to evaluate neurogenesis in the peri-infarct region. The nuclei were counter-stained with DAPI. Asterisk represents the significant difference from vehicle (n = 8/group; p < 0.05). Pond represents the significant difference from 1 mg group (n = 8/group; p < 0.05). Scale bar = 25 μm.
  

  Panel A shown only. Rat brain sections were first pre-treated with citrate buffer (0.01 mol/L, pH 6.0) for 5 minutes at 85 °C, followed by incubation with 5% normal goat serum for 1 hour at room temperature. Sections were then incubated with anti-Ki67 (rabbit monoclonal antibody to Ki67, 1/500; Anti-Ki67 antibody [SP6] ab16667) and anti-NeuN (rabbit monoclonal antibody to NeuN, 1/500; ab190565), overnight at 4°C. Sections were then rinsed in phosphate-buffered saline 3 times, followed by incubation with rabbit secondary antibody (anti-rabbit IgG, 1/1000) for 1 hour at room temperature. Fluorescence signals were then detected under a microscope. Negative control was conducted by incubating sections with PBS instead of primary antibodies. No positive signals were shown in negative control.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  IHC image of ab190565 staining in formalin fixed paraffin embedded tissue section of normal human cerebellum.

  The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with ab190565 (1/50) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then counterstained and mounted with SlowFade^® Gold Antifade Mountant with DAPI.

  The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor^® 647 signal is dervied directly from bound ab190565. The separate images of ab190565 and DAPI alone, combined with the merged version of both signals, shows predominant co-localisation of the Alexa Fluor^® 647 signal in the nuclei of the cerebellar granule layer.

  For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  Immunohistochemistry (Frozen sections) analysis of mouse cerebellum tissue sections labeling NeuN with Purified ab190565 at 1/1000 (2.0 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  ab190565 staining NeuN in U87-MG cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab190565 at 1/50 dilution(shown in red) and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Alexa Fluor^® 488 Goat anti-Mouse secondary (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  Immunohistochemistry (Frozen sections) analysis of rat cerebellum tissue sections labeling NeuN with Purified ab190565 at 1/1000 (2.0 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  ab190565 staining NeuN in NGF-differentiated PC12 cells (7 days). The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab190565 at 1/50 dilution (shown in red) and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Alexa Fluor^® 488 Goat anti-Mouse secondary (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  IHC image of ab190565 staining in formalin fixed paraffin embedded tissue section of normal rat cerebellum.

  The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with ab190565 (1/50) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then counterstained and mounted with SlowFade^® Gold Antifade Mountant with DAPI.

  The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor^® 647 signal is dervied directly from bound ab190565. The separate images of ab190565 and DAPI alone, combined with the merged version of both signals, shows predominant co-localisation of the Alexa Fluor^® 647 signal in the nuclei of the cerebellar granule layer.

  For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  IHC image of ab190565 staining in formalin fixed paraffin embedded tissue section of normal mouse cerebellum.

  The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with ab190565 (1/50) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then counterstained and mounted with SlowFade^® Gold Antifade Mountant with DAPI.

  The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor^® 647 signal is dervied directly from bound ab190565. The separate images of ab190565 and DAPI alone, combined with the merged version of both signals, shows predominant co-localisation of the Alexa Fluor^® 647 signal in the nuclei of the cerebellar granule layer.

  For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Mouse hippocampus (fresh frozen) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh frozen) tissue labeling Dynorphin A with Anti-Dynorphin A antibody [EPR28632-17] ab319045 at 1/50 (10.2 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Dynorphin A (Anti-Dynorphin A antibody [EPR28632-17] ab319045, green), anti-NeuN (ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, magenta) on mouse hippocampus.
  
  Panel B: anti-Dynorphin A stained on mouse hippocampus.
  
  Panel C: anti-NeuN stained in neurons of mouse hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of mouse hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-Dynorphin A antibody [EPR28632-17] ab319045 and ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Rat hippocampus (fresh frozen) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh frozen) tissue labeling Dynorphin A with Anti-Dynorphin A antibody [EPR28632-17] ab319045 at 1/50 (10.2 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Dynorphin A (Anti-Dynorphin A antibody [EPR28632-17] ab319045, green), anti-NeuN (ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, magenta) on rat hippocampus.
  
  Panel B: anti-Dynorphin A stained on rat hippocampus.
  
  Panel C: anti-NeuN stained in neurons of rat hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of rat hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-Dynorphin A antibody [EPR28632-17] ab319045 and ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

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  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Rat hippocampus (perfused fixed) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (perfused fixed) tissue labeling ALDH1L1 with Anti-ALDH1L1 antibody [RM1230] - Astrocyte Marker ab321905 at 1/100 (5.16 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Aldh1l1 (Anti-ALDH1L1 antibody [RM1230] - Astrocyte Marker ab321905, green), anti-NeuN (ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, red) on rat hippocampus.
  
  Panel B: anti-Aldh1l1 stained on rat hippocampus.
  
  Panel C: anti-NeuN stained in neurons of rat hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of rat hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-ALDH1L1 antibody [RM1230] - Astrocyte Marker ab321905 and ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Mouse hippocampus (perfused fixed) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (perfused fixed) tissue labeling ALDH1L1 with Anti-ALDH1L1 antibody [RM1230] - Astrocyte Marker ab321905 at 1/100 (5.16 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Aldh1l1 (Anti-ALDH1L1 antibody [RM1230] - Astrocyte Marker ab321905, green), anti-NeuN (ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, red) on mouse hippocampus.
  
  Panel B: anti-Aldh1l1 stained on mouse hippocampus.
  
  Panel C: anti-NeuN stained in neurons of mouse hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of mouse hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-ALDH1L1 antibody [RM1230] - Astrocyte Marker ab321905 and ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Rat cerebellum (perfused fixed) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (perfused fixed) tissue labeling ER81/ETV1 with Anti-ER81/ETV1 antibody [DZR-1-29] ab322139 at 1/50 (9.94 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-ETV1 (Anti-ER81/ETV1 antibody [DZR-1-29] ab322139, green), anti-NeuN (ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, magenta) on rat cerebellum.
  
  Panel B: anti-ETV1 stained on rat cerebellum.
  
  Panel C: anti-NeuN stained in neurons of rat cerebellum.
  
  Panel D: anti-GFAP stained in astrocytes of rat cerebellum.
  
  The section was incubated in two rounds of staining: in the order of Anti-ER81/ETV1 antibody [DZR-1-29] ab322139 and ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Mouse cerebellum using rabbit Anti-NeuN antibody

  Image A: composite multiplex immunohistochemistry staining using conjugates panel composed of anti-P2Y12 (Alexa Fluor^® 488) (Alexa Fluor® 488 Anti-P2Y12 antibody [EPR26298-93] ab309607, 1/50 dilution, 10 µg/mL) (green), anti-GFAP (Alexa Fluor^® 555) (Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201735, 1/100 dilution, 5 µg/mL) (magenta) and anti-NeuN (Alexa Fluor^® 647) (ab190565, 1/100 dilution, 5 µg/mL) (white) on Frozen sections of mouse cerebellum. Nuclear DNA was labelled with DAPI (blue).

  Image B: Frozen section of mouse cerebellum stained with anti- P2Y12 (Alexa Fluor^® 488) (Alexa Fluor® 488 Anti-P2Y12 antibody [EPR26298-93] ab309607, 1/50 dilution, 10 µg/mL).

  Image C: Frozen section of mouse cerebellum stained with anti-GFAP (Alexa Fluor^® 555) (Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201735, 1/100 dilution, 5 µg/mL).

  Image D: Frozen section of mouse cerebellum stained with anti-NeuN (Alexa Fluor^® 647) (ab190565, 1/100 dilution, 5 µg/mL).

  Immunohistochemistry staining was performed on a Leica Biosystems BOND^® RX instrument. The section was initially fixed using 10% formaldehyde in 1XPBS for 10 minutes. The section was then incubated at room temperature for 1 hour and mounted using Dako Fluorescence Mounting Medium^®.

  Image acquisition was performed with Leica SP8 confocal microscope. For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Mouse cerebellum (perfused-fixed) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized Mouse cerebellum (perfused-fixed) tissue labeling Ppp1r17 with Anti-Ppp1r17 antibody [EPR29124-227] ab317831 at a 1/500 (1.046 µg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at a 1/1000 (2 µg/mL) dilution.

  Panel A: merged staining of anti-Ppp1r17 (Anti-Ppp1r17 antibody [EPR29124-227] ab317831, green), anti-NeuN (ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, magenta) on mouse cerebellum.
  Panel B: anti-Ppp1r17 stained on mouse cerebellum.
  Panel C: anti-NeuN stained in neurons of mouse cerebellum.
  Panel D: anti-GFAP stained in astrocytes of mouse cerebellum.

  The section was incubated in two rounds of staining: in the order of Anti-Ppp1r17 antibody [EPR29124-227] ab317831 and ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Rat cerebellum (perfused-fixed) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized Rat cerebellum (perfused-fixed) tissue labeling Ppp1r17 with Anti-Ppp1r17 antibody [EPR29124-227] ab317831 at a 1/500 (1.046 µg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at a 1/1000 (2 µg/mL) dilution.

  Panel A: merged staining of anti-Ppp1r17 (Anti-Ppp1r17 antibody [EPR29124-227] ab317831, green), anti-NeuN (ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, magenta) on rat cerebellum.
  Panel B: anti-Ppp1r17 stained on rat cerebellum.
  Panel C: anti-NeuN stained in neurons of rat cerebellum.
  Panel D: anti-GFAP stained in astrocytes of rat cerebellum.

  The section was incubated in two rounds of staining: in the order of Anti-Ppp1r17 antibody [EPR29124-227] ab317831 and ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Mouse hippocampus (perfused fixed) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (perfused fixed) tissue labeling SCRN1 with Anti-SCRN1 antibody [EPR29587-538] ab323711 at 150 (9.96 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Secernin-1 (Anti-SCRN1 antibody [EPR29587-538] ab323711, green), anti-NeuN (ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, magenta) on mouse hippocampus.
  
  Panel B: anti-Secernin-1stained on mouse hippocampus.
  
  Panel C: anti-NeuN stained in neurons of mouse hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of mouse hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-SCRN1 antibody [EPR29587-538] ab323711 and ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Rat hippocampus (perfused fixed) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (perfused fixed) tissue labeling SCRN1 with Anti-SCRN1 antibody [EPR29587-538] ab323711 at 150 (9.96 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Secernin-1 (Anti-SCRN1 antibody [EPR29587-538] ab323711, green), anti-NeuN (ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, magenta) on rat hippocampus.
  
  Panel B: anti-Secernin-1 stained on rat hippocampus.
  
  Panel C: anti-NeuN stained in neurons of rat hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of rat hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-SCRN1 antibody [EPR29587-538] ab323711 and ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Mouse hippocampus (fresh frozen) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh frozen) tissue labeling Synaptojanin with Anti-Synaptojanin antibody [EPR28576-183] ab323848 at 1/50 (10.62 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Synaptojanin-1 (Anti-Synaptojanin antibody [EPR28576-183] ab323848, green), anti-NeuN (ab190565, white) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, magenta) on mouse hippocampus.
  
  Panel B: anti-Synaptojanin-1 stained on mouse hippocampus.
  
  Panel C: anti-NeuN stained in neurons of mouse hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of mouse hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-Synaptojanin antibody [EPR28576-183] ab323848 and ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Rat hippocampus (fresh frozen) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh frozen) tissue labeling Synaptojanin with Anti-Synaptojanin antibody [EPR28576-183] ab323848 at 1/50 (10.62 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Synaptojanin-1 (Anti-Synaptojanin antibody [EPR28576-183] ab323848, green), anti-NeuN (ab190565, white) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, magenta) on rat hippocampus.
  
  Panel B: anti-Synaptojanin-1 stained on rat hippocampus.
  
  Panel C: anti-NeuN stained in neurons of rat hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of rat hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-Synaptojanin antibody [EPR28576-183] ab323848 and ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Mouse hippocampus (fresh frozen) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh frozen) tissue labeling Neogenin with Anti-Neogenin antibody [EPR30444-522] ab324107 at 1/50 (9.76 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Neogenin (Anti-Neogenin antibody [EPR30444-522] ab324107, green), anti-NeuN (ab190565, white) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, magenta) on mouse hippocampus.
  
  Panel B: anti-Neogenin stained on mouse hippocampus.
  
  Panel C: anti-NeuN stained in neurons of mouse hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of mouse hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-Neogenin antibody [EPR30444-522] ab324107 and ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Mouse cerebrum (fresh frozen) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh frozen) tissue labeling Agrin with Anti-Agrin antibody [RM2082] ab324022 at 1/50 (9.98 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-AGRN (Anti-Agrin antibody [RM2082] ab324022, green), anti-NeuN (ab190565, white) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, magenta) on mouse cerebrum.
  
  Panel B: anti-AGRN stained in endothelial cells on mouse cerebrum.
  
  Panel C: anti-NeuN stained in neurons of mouse cerebrum.
  
  Panel D: anti-GFAP stained in astrocytes of mouse cerebrum.
  
  The section was incubated in two rounds of staining: in the order of Anti-Agrin antibody [RM2082] ab324022 and ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Rat hippocampus (fresh frozen) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh frozen) tissue labeling Neogenin with Anti-Neogenin antibody [EPR30444-522] ab324107 at 1/50 (9.76 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Neogenin (Anti-Neogenin antibody [EPR30444-522] ab324107, green), anti-NeuN (ab190565, white) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, magenta) on rat hippocampus.
  
  Panel B: anti-Neogenin stained on rat hippocampus.
  
  Panel C: anti-NeuN stained in neurons of rat hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of rat hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-Neogenin antibody [EPR30444-522] ab324107 and ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Rat cerebrum (fresh frozen) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh frozen) tissue labeling SUN1 with Anti-SUN1 antibody [EPR29563-76] ab323868 at 1/50 (10.2 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-SUN1 (Anti-SUN1 antibody [EPR29563-76] ab323868, green), anti-NeuN (ab190565, magenta) on rat cerebrum.
  
  Panel B: anti-SUN1 stained on rat cerebrum.
  
  Panel C: anti-NeuN stained in neurons of rat cerebrum.
  
  The section was incubated in two rounds of staining: in the order of Anti-SUN1 antibody [EPR29563-76] ab323868 and ab190565, for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Mouse cerebrum (fresh frozen) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh frozen) tissue labeling SUN1 with Anti-SUN1 antibody [EPR29563-76] ab323868 at 1/50 (10.2 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-SUN1 (Anti-SUN1 antibody [EPR29563-76] ab323868, green), anti-NeuN (ab190565, magenta) on mouse cerebrum.
  
  Panel B: anti-SUN1 stained on mouse cerebrum.
  
  Panel C: anti-NeuN stained in neurons of mouse cerebrum.
  
  The section was incubated in two rounds of staining: in the order of Anti-SUN1 antibody [EPR29563-76] ab323868 and ab190565, for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh frozen) tissue labeling nNOS (neuronal) with Anti-nNOS (neuronal) antibody [RM1237] ab323183 at 1/100 (5.05 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

  Panel A: merged staining of anti-nNOS (neuronal) (Anti-nNOS (neuronal) antibody [RM1237] ab323183, green), anti-Synapsin I (Alexa Fluor® 594 Anti-Synapsin I - Synaptic Marker antibody [EPR23531-50] ab309972, magenta) and anti-NeuN (ab190565, ) on rat cerebellum.
  
  Panel B: anti-nNOS (neuronal) stained on rat cerebellum.
  
  Panel C: anti-Synapsin I stained in neurons of rat cerebellum.
  
  Panel D: anti-NeuN stained in neurons of rat cerebellum.
  
  The section was incubated in two rounds of staining: in the order of Anti-nNOS (neuronal) antibody [RM1237] ab323183 and Alexa Fluor® 594 Anti-Synapsin I - Synaptic Marker antibody [EPR23531-50] ab309972, ab190565 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh frozen) tissue labeling nNOS (neuronal) with Anti-nNOS (neuronal) antibody [RM1237] ab323183 at 1/100 (5.05 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

  Panel A: merged staining of anti-nNOS (neuronal) (Anti-nNOS (neuronal) antibody [RM1237] ab323183, green), anti-Synapsin I (Alexa Fluor® 594 Anti-Synapsin I - Synaptic Marker antibody [EPR23531-50] ab309972, magenta) and anti-NeuN (ab190565, ) on mouse cerebellum.
  
  Panel B: anti-nNOS (neuronal) stained on mouse cerebellum.
  
  Panel C: anti-Synapsin I stained in neurons of mouse cerebellum.
  
  Panel D: anti-NeuN stained in neurons of mouse cerebellum.
  
  The section was incubated in two rounds of staining: in the order of Anti-nNOS (neuronal) antibody [RM1237] ab323183 and Alexa Fluor® 594 Anti-Synapsin I - Synaptic Marker antibody [EPR23531-50] ab309972, ab190565 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse dorsal root ganglion (fresh frozen) tissue labeling SCN11A with Anti-SCN11A antibody [EPR28603-66] ab316324 at 1/200 (2.455 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

  Panel A: merged staining of anti-SCN11A (Anti-SCN11A antibody [EPR28603-66] ab316324, green) and anti-NeuN (ab190565, magenta) on mouse dorsal root ganglia.
  
  Panel B: anti-SCN11A stained on mouse dorsal root ganglia.
  
  Panel C: anti-NeuN stained in neurons of mouse dorsal root ganglia.
  
  The section was incubated in two rounds of staining: in the order of Anti-SCN11A antibody [EPR28603-66] ab316324 and ab190565 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse dorsal root ganglion (fresh) tissue labeling Kv4.2/KCND2 with Anti-Kv4.2/KCND2 antibody [EPR26384-89] ab307710 at 1/100 (5.32 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Panel A: merged staining of anti-Kv4.2 (Anti-Kv4.2/KCND2 antibody [EPR26384-89] ab307710, green) and anti-NeuN (ab190565, red) on mouse dorsal root ganglion.Panel B: anti-Kv4.2 stained on the mouse dorsal root ganglion.Panel C: anti-NeuN stained in neurons of mouse dorsal root ganglion.Negative control: dorsal root ganglion (PMID: 19668710).The nuclear counterstain was DAPI (Blue). The section was incubated in two rounds of staining: in the order of Anti-Kv4.2/KCND2 antibody [EPR26384-89] ab307710 and ab190565 for 1 hr at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh) tissue labeling Kv4.2/KCND2 with Anti-Kv4.2/KCND2 antibody [EPR26384-89] ab307710 at 1/100 (5.32 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Panel A: merged staining of anti-Kv4.2 (Anti-Kv4.2/KCND2 antibody [EPR26384-89] ab307710, green) and anti-NeuN (ab190565, red) on rat cerebellum.Panel B: anti-Kv4.2 stained on the rat cerebellum.Panel C: anti-NeuN stained in neurons of rat cerebellum.The nuclear counterstain was DAPI (Blue). The section was incubated in two rounds of staining: in the order of Anti-Kv4.2/KCND2 antibody [EPR26384-89] ab307710 and ab190565 for 1 hr at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh) tissue labeling Kv4.2/KCND2 with Anti-Kv4.2/KCND2 antibody [EPR26384-89] ab307710 at 1/100 (5.32 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Panel A: merged staining of anti-Kv4.2 (Anti-Kv4.2/KCND2 antibody [EPR26384-89] ab307710, green) and anti-NeuN (ab190565, red) on mouse cerebellum.Panel B: anti-Kv4.2 stained on the mouse cerebellum.Panel C: anti-NeuN stained in neurons of mouse cerebellum.The nuclear counterstain was DAPI (Blue). The section was incubated in two rounds of staining: in the order of Anti-Kv4.2/KCND2 antibody [EPR26384-89] ab307710 and ab190565 for 1 hr at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat dorsal root ganglion (fresh) tissue labeling Kv4.2/KCND2 with Anti-Kv4.2/KCND2 antibody [EPR26384-89] ab307710 at 1/100 (5.32 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Panel A: merged staining of anti-Kv4.2 (Anti-Kv4.2/KCND2 antibody [EPR26384-89] ab307710, green) and anti-NeuN (ab190565, red) on rat dorsal root ganglion.Panel B: anti-Kv4.2 stained on the rat dorsal root ganglion.Panel C: anti-NeuN stained in neurons of rat dorsal root ganglion.Negative control: dorsal root ganglion (PMID: 19668710).The nuclear counterstain was DAPI (Blue). The section was incubated in two rounds of staining: in the order of Anti-Kv4.2/KCND2 antibody [EPR26384-89] ab307710 and ab190565 for 1 hr at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cortex (fresh) tissue labeling TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at 1/100 dilution (5.12 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
  Panel A: merged staining of anti-TRIM46 (Anti-TRIM46 antibody [EPR26957-34] ab307967, green) and anti-NeuN (ab190565, red) on mouse cortex.
  Panel B: anti-TRIM46 stained on the mouse cortex.
  Panel C: anti-NeuN stained in neurons of mouse cortex.
  The section was incubated in two rounds of staining: in the order of Anti-TRIM46 antibody [EPR26957-34] ab307967 and ab190565 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cortex (fresh) tissue labeling TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at 1/100 dilution (5.12 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
  Panel A: merged staining of anti-TRIM46 (Anti-TRIM46 antibody [EPR26957-34] ab307967, green) and anti-NeuN (ab190565, red) on rat cortex.
  Panel B: anti-TRIM46 stained on the rat cortex.
  Panel C: anti-NeuN stained in neurons of rat cortex.
  The section was incubated in two rounds of staining: in the order of Anti-TRIM46 antibody [EPR26957-34] ab307967 and ab190565 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Mouse striatum (fresh frozen) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse striatum (fresh frozen) tissue labeling SV2C with ab324233 at 1/50 (10.36 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

  Panel A: merged staining of anti-SV2C (ab324233, green), anti-NeuN (ab190565, white) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, magenta) on mouse striatum.
  
  Panel B: anti-SV2C stained on mouse striatum.
  
  Panel C: anti-NeuN stained in neurons of mouse striatum.
  
  Panel D: anti-GFAP stained in astrocytes of mouse cerebrum.
  
  The section was incubated in two rounds of staining: in the order of ab324233 and ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver (fresh) tissue labeling Nav1.8/SCN10A with Anti-Nav1.8/SCN10A antibody [EPR25132-222] ab307817 at 1/50 dilution (10.64 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).
  Negative control: confocal image showing no staining on rat liver (PMID: 34409080).
  The section was incubated in two rounds of staining: in the order of Anti-Nav1.8/SCN10A antibody [EPR25132-222] ab307817 and ab190565 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Rat striatum (fresh frozen) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat striatum (fresh frozen) tissue labeling SV2C with ab324233 at 1/50 (10.36 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

  Panel A: merged staining of anti-SV2C (ab324233, green), anti-NeuN (ab190565, white) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, magenta) on rat striatum.
  
  Panel B: anti-SV2C stained on rat striatum.
  
  Panel C: anti-NeuN stained in neurons of mouse striatum.
  
  Panel D: anti-GFAP stained in astrocytes of rat cerebrum.
  
  The section was incubated in two rounds of staining: in the order of ab324233 and ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Mouse hippocampus (perfused fixed) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (perfused fixed) tissue labeling GABRA4 with Anti-GABRA4 antibody [EPR28365-209] ab323502 at 1/50 (10.24 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-GABRA4 (Anti-GABRA4 antibody [EPR28365-209] ab323502, green), anti-NeuN (ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, magenta) on mouse hippocampus.
  
  Panel B: anti-GABRA4 stained on mouse hippocampus.
  
  Panel C: anti-NeuN stained in neurons of mouse hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of mouse hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-GABRA4 antibody [EPR28365-209] ab323502 and ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Rat hippocampus (perfused fixed) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (perfused fixed) tissue labeling GABRA4 with Anti-GABRA4 antibody [EPR28365-209] ab323502 at 1/50 (10.24 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-GABRA4 (Anti-GABRA4 antibody [EPR28365-209] ab323502, green), anti-NeuN (ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, magenta) on rat hippocampus.
  
  Panel B: anti-GABRA4 stained on rat hippocampus.
  
  Panel C: anti-NeuN stained in neurons of rat hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of rat hippocampus.
  
  The section was incubated in two rounds of staining: in the order of Anti-GABRA4 antibody [EPR28365-209] ab323502 and ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (AlexaFluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

• 

  Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

  NeuN Immunohistochemistry (Frozen sections) staining of Mouse hippocmapus (fresh frozen) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocmapus (fresh frozen) tissue labeling Munc18-1 with Anti-Munc18-1 antibody [EPR27956-86] ab315893 at 1/50 (9.8 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-Munc18-1 (Anti-Munc18-1 antibody [EPR27956-86] ab315893, green), anti-NeuN (ab190565, grey) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, red) on mouse hippocampus.
  
  Panel B: anti-Munc18-1 stained on mouse hippocampus.
  
  Panel C: anti-NeuN stained in neurons of mouse hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of mouse hippocampus.
  
  The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Munc18-1 antibody [EPR27956-86] ab315893 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.