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AB309245

Alexa Fluor® 647 Anti-NeuroD1 antibody [EPR17084]

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Rabbit Recombinant Monoclonal NeuroD1 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-Fr and reacts with Mouse samples.

View Alternative Names

BHLHA3, NEUROD, NEUROD1, Neurogenic differentiation factor 1, NeuroD, NeuroD1, Class A basic helix-loop-helix protein 3, bHLHa3

2 Images
Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuroD1 antibody [EPR17084] (AB309245)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuroD1 antibody [EPR17084] (AB309245)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh) tissue labelling NeuroD1 with ab309245 at 1/50 (10.0 ug/ml) dilution (Green). Confocal image showing positive staining on mouse hippocampus. The nuclear counterstain was DAPI (Blue). The section was incubated with ab309245 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuroD1 antibody [EPR17084] (AB309245)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuroD1 antibody [EPR17084] (AB309245)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labelling NeuroD1 with ab309245 at 1/50 (10.0 ug/ml) dilution (Green). Negative control : confocal image showing no staining on mouse liver (PMID : 24309898). The nuclear counterstain was DAPI (Blue). The section was incubated with ab309245 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17084

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Mouse

Applications

IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NeuroD1 also referred to as Neurogenic Differentiation 1 is a basic helix-loop-helix transcription factor with a molecular mass of approximately 40 kDa. This protein is expressed mainly in the central nervous system as well as in the pancreas. NeuroD1 plays a role in activating transcription by binding to DNA sequences in specific target genes. This process involves dimerization with E proteins facilitating its function as a transcriptional activator.
Biological function summary

NeuroD1 influences neural development and differentiation. It is vital in converting undifferentiated neural precursors into mature neurons ensuring proper brain formation. NeuroD1 forms part of a transcriptional complex with other neural transcription factors which coordinates the expression of genes needed for neuron specification. Apart from neurogenesis it regulates insulin gene expression in pancreatic beta cells linking neural functions to endocrine regulation.

Pathways

NeuroD1 functions in the neurogenesis pathway and interacts closely with the Notch signaling pathway. It plays a role by influencing the expression of genes essential for neuronal lineage commitment. In the insulin signaling pathway NeuroD1 cooperates with other transcription factors like Pdx1 and MafA managing the expression of insulin and other important beta-cell genes. Interactions with proteins like E47 increase its regulatory effects on these pathways.

Defects in NeuroD1 function connect to conditions such as diabetes mellitus and medulloblastoma. In diabetes mellitus NeuroD1 mutations can disturb insulin gene regulation impacting glucose metabolism severely. The connection with Pdx1 further magnifies its involvement in pancreatic beta-cell function. In medulloblastoma aberrant expression or mutations in NeuroD1 affect neuronal differentiation leading to tumor development. NeuroD1 influences cellular pathways interacting with proteins such as Sox9 which contributes to oncogenic processes in neural tissues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a transcriptional activator : mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis (By similarity).
See full target information NEUROD1
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Complementary Products

Proteins & Peptides

AB211934

Recombinant Human NeuroD1 protein

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Product promise

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