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Rabbit Recombinant Monoclonal nkx6.1 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human, Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-nkx6.1 antibody [EPR20405] (AB322233), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-nkx6.1 antibody [EPR20405] (AB322233), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-nkx6.1 antibody [EPR20405] (AB322233), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-nkx6.1 antibody [EPR20405] (AB322233), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested
Mouse
Tested

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in the development of insulin-producing beta cells in the islets of Langerhans at the secondary transition (By similarity). Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals (By similarity).

Alternative names

NKX6A, NKX6-1, Homeobox protein Nkx-6.1, Homeobox protein NK-6 homolog A

Rabbit Recombinant Monoclonal nkx6.1 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR20405
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Nkx6.1 is a homeodomain-containing transcription factor also known as NKX6A. It weighs approximately 36 kDa and is mainly expressed in the developing central nervous system and pancreas. In the pancreas it is found during early developmental stages in islet and exocrine cells. The protein acts as a transcriptional regulator and influences the expression of genes important for cell differentiation and development.

Biological function summary

Nkx6.1 plays a significant role in pancreatic beta-cell lineage development. It regulates genes that are essential for maintaining beta-cell identity and functions such as insulin gene transcription. Nkx6.1 functions as a part of a transcription factor complex and works in conjunction with other proteins to achieve its effects. It upregulates target genes necessary for cell cycle progression and insulin regulation ensuring proper beta-cell function.

Pathways

Nkx6.1 is strongly associated with the pancreatic development pathway and cell differentiation processes. It works closely with proteins like Pax4 and Pax6 to control the fate of pancreatic progenitor cells. The Notch signaling pathway also involves Nkx6.1 where it influences the determination of specific cell types in the pancreas. This interaction helps regulate the balance between proliferation and differentiation of precursor cells.

Associated diseases and disorders

Nkx6.1 has connections to diabetes especially type 1 diabetes as it affects insulin-producing beta-cells. A deficiency or malfunction in Nkx6.1 can lead to impaired insulin synthesis and secretion. Nkx2.2 is another protein involved in the pancreatic beta-cell function and shares regulatory pathways with Nkx6.1. Additionally research hints at Nkx6.1 involvement in certain central nervous system disorders since alterations in its expression could result in developmental abnormalities.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-nkx6.1 antibody [EPR20405] (ab322233), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-nkx6.1 antibody [EPR20405] (ab322233)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling nkx6.1 with ab322233 at 1/50 (10.0 ug/ml) dilution.

    Nuclear staining on islet of human pancreas?

    The section was incubated with ab322233 for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-nkx6.1 antibody [EPR20405] (ab322233), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-nkx6.1 antibody [EPR20405] (ab322233)

    Immunohistochemical analysis of paraffin-embedded Human pancreas cancer tissue labeling nkx6.1 with ab322233 at 1/50 (10.0 ug/ml) dilution.

    Nuclear staining on tumor cells of human pancreatic cancer.

    The section was incubated with ab322233 for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-nkx6.1 antibody [EPR20405] (ab322233), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-nkx6.1 antibody [EPR20405] (ab322233)

    Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling nkx6.1 with ab322233 at 1/50 (10.0 ug/ml) dilution.

    Nuclear staining on islet of mouse pancreas.

    The section was incubated with ab322233 for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-nkx6.1 antibody [EPR20405] (ab322233), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-nkx6.1 antibody [EPR20405] (ab322233)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling nkx6.1 with ab322233 at 1/50 (10.0 ug/ml) dilution.

    Negative control: no staining on human colon.

    The section was incubated with ab322233 for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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