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AB198154

Alexa Fluor® 647 Anti-NNT antibody [8B4BB10]

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(1 Publication)

Mouse Monoclonal NNT antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Nicotinamide nucleotide transhydrogenase, Pyridine nucleotide transhydrogenase, NNT

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NNT antibody [8B4BB10] (AB198154)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NNT antibody [8B4BB10] (AB198154)

ab198154 staining NNT in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab198154 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8B4BB10

Isotype

IgG1

Light chain type

kappa

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Proprietary technique
Purification notes
Produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nicotinamide nucleotide transhydrogenase (NNT) is an enzyme that plays an important role in cellular redox balance by catalyzing the hydride transfer between NADH and NADP+ across the inner mitochondrial membrane. Also known as NNT in common scientific references the enzyme has a mass of approximately 109 kDa. NNT is primarily expressed in tissues with high metabolic demand such as the liver heart and adrenal glands. Its high expression in the mitochondria reflects its essential role in maintaining efficient energy homeostasis.
Biological function summary

NNT regulates the conversion of NADP+ to NADPH a form that serves as an important reducing agent in cellular mechanisms. The enzyme does not function alone but integrates into the mitochondrial membrane working as a part of a complex with other mitochondrial proteins like ATP synthase and cytochrome oxidase. This role is important for antioxidant defense as NADPH is utilized in pathways that mitigate reactive oxygen species maintaining cellular integrity.

Pathways

NNT is a central component in energy metabolism and detoxification processes. It is intricately involved in the pentose phosphate pathway where it helps generate reducing equivalents in the form of NADPH. Additionally NNT is linked to the oxidative phosphorylation pathway ensuring efficient ATP production. The enzyme's activity is closely associated with proteins such as glutathione reductase and thioredoxin which rely on the reducing power of NADPH provided by NNT for their antioxidant functions.

NNT mutations have been associated with familial glucocorticoid deficiency and metabolic disorders which highlight its importance in endocrine and energy balance. Altered NNT activity has been observed in various cases of hypertension and heart failure. The enzyme's interrelationship with proteins like cortisol which is important in adrenal function and insulin in relation to metabolic processes and regulation indicate its broad influence in both energy homeostasis and redox balance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane (By similarity). May play a role in reactive oxygen species (ROS) detoxification in the adrenal gland (PubMed : 22634753).
See full target information NNT
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:5788 PubMed37031254

2023

Possible frequent multiple mitochondrial DNA copies in a single nucleoid in HeLa cells.

Applications

Unspecified application

Species

Unspecified reactive species

Vojtěch Pavluch,Tomáš Špaček,Hana Engstová,Andrea Dlasková,Petr Ježek
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Product promise

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