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AB270116

Alexa Fluor® 647 Anti-NR2F2 antibody [EPR18443]

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Rabbit Recombinant Monoclonal NR2F2 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, IHC-P and reacts with Mouse, Human, Rat samples.

View Alternative Names

ARP1, TFCOUP2, NR2F2, COUP transcription factor 2, COUP-TF2, Apolipoprotein A-I regulatory protein 1, COUP transcription factor II, Nuclear receptor subfamily 2 group F member 2, ARP-1, COUP-TF II

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NR2F2 antibody [EPR18443] (AB270116)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NR2F2 antibody [EPR18443] (AB270116)

Immunohistochemistry analysis of paraffin-embedded sections human breast cancer tissue labeling NR2F2 with ab270116 at 1/50 dilution, incubated at 4°C overnight (shown in magenta), followed by an Autofluorescence blocker Kit according to its datasheet. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0). Nuclear staining on tumor cells of human breast cancer. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NR2F2 antibody [EPR18443] (AB270116)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NR2F2 antibody [EPR18443] (AB270116)

Immunohistochemistry analysis of paraffin-embedded sections human testis tissue labeling NR2F2 with ab270116 at 1/50 dilution, incubated at 4°C overnight (shown in magenta), followed by an Autofluorescence blocker Kit according to its datasheet. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0). Nuclear staining on mesenchymal cells of human testis. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NR2F2 antibody [EPR18443] (AB270116)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NR2F2 antibody [EPR18443] (AB270116)

ab270116 staining NR2F2 in NIH/3T3 (Mouse embryo fibroblast cell line) cells.

The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab270116 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor®488), at 1/250 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NR2F2 antibody [EPR18443] (AB270116)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NR2F2 antibody [EPR18443] (AB270116)

Immunohistochemistry analysis of paraffin-embedded sections mouse liver tissue labeling NR2F2 with ab270116 at 1/50 dilution, incubated at 4°C overnight (shown in magenta), followed by an Autofluorescence blocker Kit according to its datasheet. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0). Nuclear staining on mesenchymal cells of mouse liver. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NR2F2 antibody [EPR18443] (AB270116)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NR2F2 antibody [EPR18443] (AB270116)

Immunohistochemistry analysis of paraffin-embedded sections rat liver tissue labeling NR2F2 with ab270116 at 1/50 dilution, incubated at 4°C overnight (shown in magenta), followed by an Autofluorescence blocker Kit according to its datasheet. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0). Nuclear staining on mesenchymal cells of rat liver. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18443

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Mouse, Human, Rat, Human, Mouse, Rat

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in NIH3T3 fixed with 4% formaldehyde (10 min).</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NR2F2 also known as COUP-TFII (Chicken Ovalbumin Upstream Promoter Transcription Factor II) is a nuclear receptor that functions as a transcription factor. It has a molecular mass of approximately 48 kDa. It binds to DNA target sequences and regulates gene expression involved in various developmental processes. NR2F2 is expressed in the heart blood vessels and various tissues such as the lungs and the central nervous system. Its role in transcriptional regulation makes it important for proper cell and tissue differentiation.
Biological function summary

NR2F2 is involved in the regulation of angiogenesis and cardiovascular development. It forms a heterodimer with retinoid X receptor (RXR) to regulate gene expression. This interaction places NR2F2 as part of a larger network controlling organ development and metabolic processes. NR2F2 activity also influences energy homeostasis and adipogenesis through its transcriptional regulation abilities.

Pathways

NR2F2 influences the Wnt signaling and Notch signaling pathways which play important roles in cell proliferation and differentiation. These pathways interact with other proteins such as beta-catenin and Notch receptors. NR2F2 modulates gene expression at different cellular stages and contributes to the precise regulation required within these pathways.

NR2F2 is linked to congenital heart defects and cancer. Aberrant NR2F2 expression disrupts normal developmental processes contributing to heart malformations. In cancer altered NR2F2 expression and function can facilitate tumor progression and metastasis often involving interaction with other proteins like TGF-beta and SMADs essential for tumor suppression pathways. The ability of NR2F2 to control critical transcriptional networks makes it a significant target for understanding and potentially treating these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A. May be required to establish ovary identity during early gonad development (PubMed : 29478779).
See full target information NR2F2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com