Mouse Monoclonal Nucleolin antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 4 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Nucleolin antibody [364-5] (AB198580), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-nucleolin-antibody-364-5-ab198580--immunocytochemistry-immunofluorescence-img76640.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Nucleolin antibody [364-5] (AB198580)")
Publications
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- Molecular cell 82:3826-3839.e92022Control of ribosomal RNA synthesis by hematopoietic transcription factors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharles Antony,Subin S George,Justin Blum,Patrick Somers,Chelsea L Thorsheim,Dexter J Wu-Corts,Yuxi Ai,Long Gao,Kaosheng Lv,Michel G Tremblay,Tom Moss,Kai Tan,Jeremy E Wilusz,Austen R D Ganley,Maxim Pimkin,Vikram R ParalkarPubMed 36113481
- Journal of visualized experiments : JoVE :2019Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeng Zhao,Yawen Tang,Patrick J Ernst,Asher Kahn-Krell,Chengming Fan,Danielle Pretorius,Hanxi Zhu,Xi Lou,Lufang Zhou,Jianyi Zhang,Wuqiang ZhuPubMed 31355804
- Frontiers in cellular neuroscience 13:2962019Distinct Conformations, Aggregation and Cellular Internalization of Different Tau Strains.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas K Karikari et. al.PubMed 31338022
- Circulation research 122:88-962017CCND2 Overexpression Enhances the Regenerative Potency of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Remuscularization of Injured Ventricle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWuqiang Zhu,Meng Zhao,Saidulu Mattapally,Sifeng Chen,Jianyi ZhangPubMed 29018036
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
Alexa Fluor® 647
- Excitation/Emission
Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
7 products for Alternative Product
Target data
Function
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Alternative names
Nucleolin, Protein C23, NCL
Recommended products
Mouse Monoclonal Nucleolin antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 4 publications.
Alternative names
Nucleolin, Protein C23, NCL
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
Alexa Fluor® 647
- Excitation/Emission
Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
364-5
- Purification technique
Affinity purification
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nucleolin also known as C23 is a multifunctional protein mainly found in the nucleolus with a molecular weight of approximately 100 kDa. It plays a mechanical role in ribosomal RNA (rRNA) synthesis and ribosome biogenesis. Nucleolin is widely expressed in proliferating cells including HeLa culture and shows higher expression in cancer cells compared to normal cells. The nucleolin protein is involved in various nucleolar processes like the modulation of chromatin structure and nucleocytoplasmic transport.
- Biological function summary
The nucleolin molecular weight protein contributes to regulating cellular processes such as chromatin remodeling rRNA transcription and mRNA stability. Nucleolin often forms complexes with various other proteins and nucleic acids enhancing its functionality within the cell nucleus. Its participation in processes like DNA replication and cell proliferation shows the central role nucleolin plays at the cellular level. It influences cellular responses by binding directly to specific RNA or DNA sequences.
- Pathways
The nucleolin protein engages in major cellular pathways such as the RNA processing and p53 signaling pathways. Through the RNA processing pathway it interacts with proteins like fibrillarin to assist in rRNA maturation. Moreover within the p53 signaling pathway nucleolin regulates the activity of the tumor suppressor protein p53 impacting cell cycle progression and apoptosis. These interactions highlight nucleolin's role in maintaining cellular homeostasis and response to stress.
- Associated diseases and disorders
The nucleolin protein often shows altered expression in cancers and cardiovascular diseases. In cancer nucleolin's high expression levels correlate with tumor progression and metastasis making it a potential target for cancer therapies. In cardiovascular diseases nucleolin interacts with proteins like VEGF to influence angiogenesis which affects disease progression. Understanding nucleolin's involvement in these diseases provides insight into potential therapeutic targets and interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Nucleolin antibody [364-5] (ab198580), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-nucleolin-antibody-364-5-ab198580--immunocytochemistry-immunofluorescence-img76640.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Nucleolin antibody [364-5] (ab198580)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Nucleolin antibody [364-5] (ab198580)
ab198580 staining Nucleolin in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198580 at 1/100 dilution (shown in red) and Anti-beta Tubulin antibody - Loading Control ab6046 (Rabbit polyclonal to beta Tubulin) at 1μg/ml. This was followed by an incubation at room temperature for 1h with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed, at 1μg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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