JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB204018

Alexa Fluor® 647 Anti-NuMA antibody [EP3976]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal NuMA antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NMP22, NUMA, NUMA1, Nuclear mitotic apparatus protein 1, Nuclear matrix protein-22, Nuclear mitotic apparatus protein, SP-H antigen, NMP-22, NuMA protein

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NuMA antibody [EP3976] (AB204018)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NuMA antibody [EP3976] (AB204018)

ab204018 staining NuMA in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204018 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP3976

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Human

Applications

IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB84680

Anti-NuMA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody (AB84680)

  • 5
  • 1
Primary Antibodies

AB241470

Anti-NuMA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody (AB241470)

  • 0
  • 0
Primary Antibodies

AB86129

Anti-NuMA antibody

BOND RX™ Validated

Immunocytochemistry - Anti-NuMA antibody (AB86129)

  • 5
  • 2
Primary Antibodies

AB97585

Anti-NuMA antibody

Immunocytochemistry/ Immunofluorescence - Anti-NuMA antibody (AB97585)

  • 3
  • 2
Primary Antibodies

AB212689

Anti-NuMA antibody [SPM300] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody [SPM300] - BSA and Azide free (AB212689)

  • 0
  • 0

Complementary Products

Proteins & Peptides

AB114324

Recombinant Human NuMA protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human NuMA protein (GST tag N-Terminus) (AB114324)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB191468

Anti-CRISPR-Cas9 antibody [7A9-3A3]

Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [7A9-3A3] (AB191468)

  • 4
  • 11
Primary Antibodies

AB18203

Anti-N Cadherin antibody - Intercellular Junction Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-N Cadherin antibody - Intercellular Junction Marker (AB18203)

  • 4
  • 48
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min).</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NuMA also known as "nuclear mitotic apparatus protein" is a large protein with a mass of approximately 250 kDa. It localizes in the nucleus and becomes highly concentrated at the spindle poles during mitosis. NuMA proteins appear in a variety of cells especially in cells undergoing division. Researchers often refer to it by alternate names such as "anti NuMA" when discussing its interactions in cellular contexts. Scientists have developed products such as NuMA polyclonal antibodies to study this protein's function.
Biological function summary

The protein plays an important role in the organization of the mitotic spindle during cell division. It ensures that the spindle microtubules are correctly aligned and positioned. NuMA acts within a complex that includes dynein and dynactin facilitating proper chromosomal segregation. These actions are critical for maintaining genomic stability through accurate cell division.

Pathways

NuMA's involvement in the cell cycle is significant especially within the mitotic checkpoint control pathways. It interacts closely with proteins like pericentrin and LGN ensuring the efficient completion of mitosis. These interactions confirm the protein's importance in pathway regulation particularly during transition phases within the cell cycle.

Abnormal NuMA function has associations with cancer and certain genetic disorders like microcephaly. Changes in NuMA expression or malfunction can disrupt normal spindle formation leading to chromosomal instability a common hallmark of cancer cells. Its interaction with proteins like p53 further connects it to oncogenic processes suggesting that understanding NuMA's pathways could contribute to therapeutic strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division (PubMed : 17172455, PubMed : 19255246, PubMed : 24996901, PubMed : 26195665, PubMed : 27462074, PubMed : 7769006). Functions to tether the minus ends of MTs at the spindle poles, which is critical for the establishment and maintenance of the spindle poles (PubMed : 11956313, PubMed : 12445386). Plays a role in the establishment of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner (PubMed : 23870127, PubMed : 24109598, PubMed : 24996901, PubMed : 26765568). In metaphase, part of a ternary complex composed of GPSM2 and G(i) alpha proteins, that regulates the recruitment and anchorage of the dynein-dynactin complex in the mitotic cell cortex regions situated above the two spindle poles, and hence regulates the correct oritentation of the mitotic spindle (PubMed : 22327364, PubMed : 23027904, PubMed : 23921553). During anaphase, mediates the recruitment and accumulation of the dynein-dynactin complex at the cell membrane of the polar cortical region through direct association with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), and hence participates in the regulation of the spindle elongation and chromosome segregation (PubMed : 22327364, PubMed : 23921553, PubMed : 24371089, PubMed : 24996901). Binds also to other polyanionic phosphoinositides, such as phosphatidylinositol 3-phosphate (PIP), lysophosphatidic acid (LPA) and phosphatidylinositol triphosphate (PIP3), in vitro (PubMed : 24371089, PubMed : 24996901). Also required for proper orientation of the mitotic spindle during asymmetric cell divisions (PubMed : 21816348). Plays a role in mitotic MT aster assembly (PubMed : 11163243, PubMed : 11229403, PubMed : 12445386). Involved in anastral spindle assembly (PubMed : 25657325). Positively regulates TNKS protein localization to spindle poles in mitosis (PubMed : 16076287). Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority of the nuclear volume (PubMed : 10075938). Required for epidermal differentiation and hair follicle morphogenesis (By similarity).
See full target information NUMA1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Ultrasonics sonochemistry 117:107339 PubMed40215791

2025

Anti-inflammatory and gut microbiota regulatory effects of ultrasonic degraded polysaccharides from Auricularia auricula-judae in DSS-induced colitis mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tahidul Islam,Baojun Xu,Zhaoxiang Bian
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com