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AB225100

Alexa Fluor® 647 Anti-Olig2 antibody [EPR2673] - Oligodendrocyte Marker

5

(1 Review)

|

(3 Publications)

Anti-Olig2 antibody [EPR2673] - Alexa Fluor® 647 conjugated (ab225100) is a rabbit recombinant monoclonal antibody detecting Olig2 in IHC-Fr. Suitable for Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

BHLHB1, BHLHE19, PRKCBP2, RACK17, OLIG2, Oligodendrocyte transcription factor 2, Oligo2, Class B basic helix-loop-helix protein 1, Class E basic helix-loop-helix protein 19, Protein kinase C-binding protein 2, Protein kinase C-binding protein RACK17, bHLHb1, bHLHe19

2 Images
Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Olig2 antibody [EPR2673] - Oligodendrocyte Marker (AB225100)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Olig2 antibody [EPR2673] - Oligodendrocyte Marker (AB225100)

Immunofluorescence staining of Olig2 in a section of frozen rat cerebellum.

The section was fixed using 10% formaldehyde in 1X PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BONDTM. The section was incubated at room temperature for 1 hour with ab225100 at 1/100 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Olig2 antibody [EPR2673] - Oligodendrocyte Marker (AB225100)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Olig2 antibody [EPR2673] - Oligodendrocyte Marker (AB225100)

Immunohistochemistry (Frozen sections) analysis of Mouse cerebrum (fresh frozen) labeling Olig2 with ab225100 at 1/50. The section was fixed using 4% PFA and permeabilised with 0.2% Triton X-100. Nuclear counterstain was DAPI. Positive staining on mouse cerebrum.

  • Unconjugated

    Anti-Olig2 antibody [EPR2673] - Oligodendrocyte Marker

  • Carrier free

    Anti-Olig2 antibody [EPR2673] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] - Oligodendrocyte Marker

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Olig2 antibody [EPR2673]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Olig2 antibody [EPR2673] - Oligodendrocyte Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Olig2 antibody [EPR2673]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2673

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Rat

Applications

IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50 - 1/100", "IHCFr-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-Olig2 antibody [EPR2673] (ab225100) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-Fr) in Mouse, Rat samples.

Related products
Antibody clone EPR2673 is also available pre-conjugated to a variety of labels for your convenience – Anti-Olig2 Alexa Fluor® 647 [EPR2673] (ab225100).

Other related products
We have a range of other formats of antibody clone [EPR2673] also available for your convenience: ab109186, Carrier free - ab220796, Alexa Fluor® 488 - ab225099, Alexa Fluor® 647 - ab225100, Alexa Fluor® 594 - ab300729

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Olig2 also known as Oligo2 or OLIG2 is a protein with a molecular mass of around 32 kDa. It belongs to the basic helix-loop-helix (bHLH) family of transcription factors which play roles in neurodevelopment. Olig2 is expressed mainly in the central nervous system particularly in oligodendrocyte progenitor cells and motor neuron progenitors. As an important marker Olig2 staining is often used in studies involving these cell types to understand differentiation processes.
Biological function summary

Olig2 regulates gene expression important for the proper development of the central nervous system. It functions both independently and as a part of transcriptional complexes to influence the fate of neural progenitors. In particular its presence decides whether these progenitors will differentiate into neurons astrocytes or oligodendrocytes. Among the proteins it interacts with are factors that modulate neurogenesis and myelination highlighting its importance in maintaining normal brain homeostasis.

Pathways

Olig2 plays an important role in the oligodendrocyte differentiation and motor neuron generation pathways. It works closely with proteins such as SOX10 and NKX2.2 to regulate oligodendrocyte maturation. In the motor neuron pathway Olig2 interacts with proteins like NKX6.1 and LHX3 coordinating the development of motor neurons. These pathways demonstrate Olig2's function as a critical transcription factor influencing neural development and plasticity.

Abnormalities in Olig2 expression or function relate to conditions such as gliomas and Multiple Sclerosis (MS). Elevated levels of Olig2 are often seen in gliomas where it might cooperate with proteins like IDH1 to drive tumor growth. In MS the impairment of oligodendrocyte function can link back to disruptions in normal Olig2 activity or expression. These associations make Olig2 a significant target for potential therapeutic interventions in neurological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Functions together with ZNF488 to promote oligodendrocyte differentiation. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
See full target information OLIG2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell 185:2184-2199.e16 PubMed35649412

2022

Glioma progression is shaped by genetic evolution and microenvironment interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Frederick S Varn,Kevin C Johnson,Jan Martinek,Jason T Huse,MacLean P Nasrallah,Pieter Wesseling,Lee A D Cooper,Tathiane M Malta,Taylor E Wade,Thais S Sabedot,Daniel Brat,Peter V Gould,Adelheid Wöehrer,Kenneth Aldape,Azzam Ismail,Santhosh K Sivajothi,Floris P Barthel,Hoon Kim,Emre Kocakavuk,Nazia Ahmed,Kieron White,Indrani Datta,Hyo-Eun Moon,Steven Pollock,Christine Goldfarb,Ga-Hyun Lee,Luciano Garofano,Kevin J Anderson,Djamel Nehar-Belaid,Jill S Barnholtz-Sloan,Spyridon Bakas,Annette T Byrne,Fulvio D'Angelo,Hui K Gan,Mustafa Khasraw,Simona Migliozzi,D Ryan Ormond,Sun Ha Paek,Erwin G Van Meir,Annemiek M E Walenkamp,Colin Watts,Tobias Weiss,Michael Weller,Karolina Palucka,Lucy F Stead,Laila M Poisson,Houtan Noushmehr,Antonio Iavarone,Roel G W Verhaak

Nature neuroscience 25:285-294 PubMed35210624

2022

Clonal relations in the mouse brain revealed by single-cell and spatial transcriptomics.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Ratz,Leonie von Berlin,Ludvig Larsson,Marcel Martin,Jakub Orzechowski Westholm,Gioele La Manno,Joakim Lundeberg,Jonas Frisén

Nature protocols 16:1629-1646 PubMed33495627

2021

Nuclei isolation of multiple brain cell types for omics interrogation.

Applications

Unspecified application

Species

Unspecified reactive species

Alexi Nott,Johannes C M Schlachetzki,Bethany R Fixsen,Christopher K Glass
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
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AB136253

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Alternative Version
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AB220796

Anti-Olig2 antibody [EPR2673] - BSA and Azide free

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Alternative Version
Primary Antibodies

AB225099

Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] - Oligodendrocyte Marker

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5

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Alternative Version
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AB300729

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Alternative Version
Primary Antibodies

AB302808

Alexa Fluor® 568 Anti-Olig2 antibody [EPR2673] - Oligodendrocyte Marker

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Alternative Version
Primary Antibodies

AB300730

Alexa Fluor® 555 Anti-Olig2 antibody [EPR2673]

primary-antibodies

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0

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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