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Rabbit Recombinant Monoclonal C-Myc/MYC antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse samples.

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Images

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-pan-myc antibody [EPR18863] (AB319080), expandable thumbnail
  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-pan-myc antibody [EPR18863] (AB319080), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-pan-myc antibody [EPR18863] (AB319080), expandable thumbnail
  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-pan-myc antibody [EPR18863] (AB319080), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)ICC/IF
Human
Tested
Not recommended
Mouse
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Species
Mouse
Dilution info
1/500 - 1/5000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Target data

Function

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed:24940000, PubMed:25956029). Activates the transcription of growth-related genes (PubMed:24940000, PubMed:25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000, PubMed:25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808).

Alternative names

BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39

Recommended products

Rabbit Recombinant Monoclonal C-Myc/MYC antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR18863
Purification technique
Affinity purification Protein A
Specificity

Unsuitable for human ICC

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Pan-MYC refers to a group of transcription factors in the MYC family primarily comprising c-Myc N-Myc and L-Myc with c-Myc weighing around 49 kDa. MYC proteins are nuclear phosphoproteins that regulate gene expression. MYC proteins express in various tissues including embryonic stem cells and proliferating cells and they are known for their involvement in cell cycle progression apoptosis and cellular transformation.

Biological function summary

MYC proteins perform an important role in the regulation of transcription by binding to DNA at E-box sequences. They function as part of the MYC/MAX/MAD network a transcriptional complex with MAX as the obligate partner. This network influences genes involved in cellular growth metabolism and differentiation. Deregulation of MYC proteins affects many cellular processes and can influence normal physiology.

Pathways

The MYC proteins are central to the regulation of the cell cycle and Wnt signaling pathways. In the PI3K/Akt signaling MYC interacts with other signaling molecules to control cell growth and survival. The E-box binding allows MYC to regulate multiple downstream targets including cyclins and growth factor receptors. MYC's ability to form a complex with MAX also interlinks it with other survival and proliferation pathways.

Associated diseases and disorders

Aberrant MYC expression correlates with various cancers such as Burkitt's lymphoma and neuroblastoma. Overexpression of MYC often results from chromosomal translocations or gene amplifications in these malignancies. MYC's connection with the BCL2 protein leads to the disruption of apoptosis promoting oncogenic transformation. Control of MYC expression and function remains a critical focus in cancer research due to its role in tumor development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-pan-myc antibody [EPR18863] (ab319080), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-pan-myc antibody [EPR18863] (ab319080)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling pan-myc with ab319080 at 1/50 dilution (1ug)(Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).

  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-pan-myc antibody [EPR18863] (ab319080), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-pan-myc antibody [EPR18863] (ab319080)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Raw 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling pan-myc with ab319080 at 1/500 dilution(0.1ug)(Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-pan-myc antibody [EPR18863] (ab319080), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-pan-myc antibody [EPR18863] (ab319080)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling pan-myc with ab319080 at 1/50 (10.0 ug/ml) dilution (Green).

    Confocal image showing nuclear staining in RAW 264.7 cells (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-pan-myc antibody [EPR18863] (ab319080), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-pan-myc antibody [EPR18863] (ab319080)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling pan-myc with ab319080 at 1/5000 dilution (0.01ug)(Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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