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AB308534

Alexa Fluor® 647 Anti-pan SCN antibody [EPR25134-4]

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Rabbit Recombinant Monoclonal SCN2A antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

HBA, NAC2, SCN2A1, SCN2A2, SCN2A, Sodium channel protein type 2 subunit alpha, HBSC II, Sodium channel protein brain II subunit alpha, Sodium channel protein type II subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.2

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-pan SCN antibody [EPR25134-4] (AB308534)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-pan SCN antibody [EPR25134-4] (AB308534)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling SCN2A with ab308534 at 1/100 (5.0 ug/ml). Positive staining on human cerebrum. The section was incubated with ab308534 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-pan SCN antibody [EPR25134-4] (AB308534)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-pan SCN antibody [EPR25134-4] (AB308534)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling SCN2A with ab308534 at 1/100 (5.0 ug/ml). Negative control : no staining on human cardiac muscle. The section was incubated with ab308534 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-pan SCN antibody [EPR25134-4] (AB308534)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-pan SCN antibody [EPR25134-4] (AB308534)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling SCN2A with ab308534 at 1/100 (5.0 ug/ml). Positive staining on mouse cerebrum. The section was incubated with ab308534 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-pan SCN antibody [EPR25134-4] (AB308534)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-pan SCN antibody [EPR25134-4] (AB308534)

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling SCN2A with ab308534 at 1/100 (5.0 ug/ml). Positive staining on mouse cerebellum. The section was incubated with ab308534 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-pan SCN antibody [EPR25134-4] (AB308534)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-pan SCN antibody [EPR25134-4] (AB308534)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling SCN2A with ab308534 at 1/100 (5.0 ug/ml). Positive staining on rat cerebrum. The section was incubated with ab308534 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25134-4

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody reacts with human SCN1A, SCN3A, and SCN9A but does not react with human SCN4A, SCN5A, SCN8A, SCN10A, and SCN11A.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target "pan SCN" also referred to as "IP SCN" and sometimes identified by "647" refers to a group encompassing several voltage-gated sodium channels commonly expressed in excitable tissues like the brain and skeletal muscles. These channels have different subunits that might have varying molecular weights but generally range around 220-260 kDa. They are essential for generating and propagating action potentials in neurons.
Biological function summary

Voltage-gated sodium channels aid in the rapid depolarization phase of the action potential in excitable cells. They open in response to membrane depolarization allowing the influx of sodium ions which is critical for the feedback cycle that continues the action potential. These channels typically comprise a core alpha subunit which forms the pore and optional accessory beta subunits that modulate functionality.

Pathways

These sodium channels integrate into critical signaling pathways like the ion homeostasis pathway and the sodium ion transport pathway. They interact with various proteins such as different isoforms of potassium channels and calcium channels to maintain the delicate balance of ionic gradients necessary for cell excitability and signaling integrity.

Mutations or dysfunctions in voltage-gated sodium channels relate strongly to conditions like epilepsy and certain skeletal muscle disorders. Epilepsy often associates with abnormal activity of the channels with related proteins such as GABA receptors and potassium channels also being implicated in the disorder's pathophysiology. Skeletal muscle disorders involve disruptions in ion channel functioning where calcium channels show significant interplay with these sodium channels in maintaining muscle contractions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient (PubMed : 1325650, PubMed : 17021166, PubMed : 28256214, PubMed : 29844171). Implicated in the regulation of hippocampal replay occurring within sharp wave ripples (SPW-R) important for memory (By similarity).
See full target information SCN2A

Additional targets

SCN1A,SCN1A,SCN9A
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Complementary Products

Proteins & Peptides

AB114727

Recombinant Human SCN2A protein

SDS-PAGE - Recombinant Human SCN2A protein (AB114727)

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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