Alexa Fluor® 647 Anti-Pancreatic Polypeptide antibody [EPR23320-10]
- RabMAb
- Recombinant
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Rabbit Recombinant Monoclonal Pancreatic Polypeptide antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Rat, Human samples.
View Alternative Names
PNP, PPY, Pancreatic polypeptide prohormone, PH, Pancreatic polypeptide Y
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB300746)](https://content.abcam.com/products/images/alexa-fluor-647-pancreatic-polypeptide-antibody-epr23320-10-ab300746--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img452566.jpg/_jcr_content/renditions/webp-768.webp)
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Pancreatic Polypeptide antibody [EPR23320-10] (AB300746)
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Pancreatic Polypeptide with ab300746 at 1/200 (2.5 µg/ml). The tissue section was first incubated with TBS containing 0.025% (v/v) Triton X-100, 0.3 M glycine and 1% (w/v) BSA for 1 h at room temperature to block non-specific protein-protein interactions, followed by overnight incubation at +4°C with ab300746 at 1/200 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Positive cytoplasmic staining was observed on rat pancreatic islets.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)
Related conjugates and formulations (1)
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Pancreatic Polypeptide antibody [EPR23320-10]
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
In various mammalian species including rats pancreatic polypeptide plays a significant role in the regulation of appetite and pancreatic secretion. It is not recognized as part of a multi-protein complex but it directly influences the function of digestive organs by inhibiting pancreatic exocrine secretions and gallbladder contraction. This makes it an important mediator in controlling the body's energy balance and digestive functions.
Pathways
Pancreatic polypeptide is involved in the peptidergic pathway regulating energy homeostasis and digestive processes. It interacts with the Y receptor family particularly the Y4 receptor which contributes to its regulatory actions on appetite and digestive enzyme secretion. This peptide works alongside other polypeptides such as neuropeptide Y and peptide YY in mediating these complex physiological pathways.
Product protocols
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Target data
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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