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AB207670

Alexa Fluor® 647 Anti-PHLDA1 antibody [EPR6674]

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(1 Publication)

Rabbit Recombinant Monoclonal PHLDA1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PHRIP, TDAG51, PHLDA1, Pleckstrin homology-like domain family A member 1, Apoptosis-associated nuclear protein, Proline- and glutamine-rich protein, Proline- and histidine-rich protein, T-cell death-associated gene 51 protein, PQ-rich protein, PQR protein

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PHLDA1 antibody [EPR6674] (AB207670)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PHLDA1 antibody [EPR6674] (AB207670)

ab207670 staining PHLDA1 in A375 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207670 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6674

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Human

Applications

IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in A375 cells fixed with 4% formaldehyde (10 min)</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PHLDA1 protein also known as T-cell death-associated gene 51 (TDAG51) weighs approximately 34 kDa. This protein plays roles in regulating apoptosis and cell proliferation. It expresses mainly in the heart kidney pancreas and placenta. PHLDA1 consists of a pleckstrin homology domain which mediates its binding to phosphoinositides allowing it to interact with cell membranes and influence cellular signaling pathways.
Biological function summary

PHLDA1 contributes to cellular processes such as apoptosis survival and proliferation. It does not appear to be part of a larger complex but engages in interactions that regulate cell fate. PHLDA1 mediates cellular response to stress and participates in cellular growth regulation by modulating the activity of other signaling molecules. This function makes it important in maintaining cellular homeostasis.

Pathways

Researchers connect PHLDA1 to the Akt signaling pathway and the TNF receptor pathway. In the Akt signaling pathway PHLDA1 interacts with proteins such as Forkhead box O3 (FOXO3) to influence cell survival and apoptosis. In the TNF receptor pathway PHLDA1 participates in modulating the balance between cell survival and programmed cell death impacting inflammatory responses.

PHLDA1 relates to cancer and neurodegenerative diseases. Its expression levels alter in certain types of cancers such as breast and prostate cancer where it may have either tumor-suppressing or oncogenic roles depending on context. In Alzheimer's disease connections exist between PHLDA1 dysregulation and proteins like amyloid precursor protein (APP) suggesting a role in disease progression. Understanding PHLDA1’s role in these conditions may offer insight into new therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Seems to be involved in regulation of apoptosis. May be involved in detachment-mediated programmed cell death. May mediate apoptosis during neuronal development. May be involved in regulation of anti-apoptotic effects of IGF1. May be involved in translational regulation.
See full target information PHLDA1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 40:1201-1209 PubMed28849073

2017

Endothelial cell-derived exosomes protect SH-SY5Y nerve cells against ischemia/reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Xiao,Yi Chai,Shigang Lv,Minhua Ye,Miaojing Wu,Liyuan Xie,Yanghua Fan,Xingen Zhu,Ziyun Gao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com