JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB205480

Alexa Fluor® 647 Anti-Phosphotyrosine antibody [EPR16871]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Phosphotyrosine antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Modified Amino Acid samples.

View Alternative Names

L-Phosphotyrosine, L-Tyrosine-O-phosphate, O-Phospho-L-tyrosine, O-Phosphotyrosine, Phospho-L-tyrosine, Phosphonotyrosine

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Phosphotyrosine antibody [EPR16871] (AB205480)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Phosphotyrosine antibody [EPR16871] (AB205480)

ab205480 staining Phosphotyrosine in C2C12 cells. The cells were incubated with 2mM H202 for 10 minutes (Treated) or solvent-only for control purposes (Non-treated). Cells were fixed with 4% formaldehyde (10min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab205480 at 1/100 dilution (shown in red) and ab195887 Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 488) at 2μg/ml (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

  • Unconjugated

    Anti-Phosphotyrosine antibody [EPR16871]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Phosphotyrosine antibody [EPR16871]

  • Biotin

    Biotin Anti-Phosphotyrosine antibody [EPR16871]

  • Carrier free

    Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16871

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphotyrosine also known simply as pY is a post-translational modification where a phosphate group binds to the hydroxyl group of a tyrosine amino acid in proteins. This small modification can have a huge impact on proteins' function and interaction. Phosphotyrosine has a molecular weight of approximately 216 Da when it stands on its own. This modification can occur in various proteins expressed widely across tissues playing a role in signaling cell division and morphological changes.
Biological function summary

Phosphorylated tyrosine residues modify the function and activity of proteins within cells. It is a critical component of signal transduction pathways and can alter protein functions when becoming part of large protein complexes. It often regulates receptor proteins and intracellular kinases modifying their ability to interact with other proteins like SH2 and PTB domain-containing proteins. Phosphotyrosine antibodies like anti-phosphotyrosine Ig2 are helpful research tools for detecting these modifications.

Pathways

The modification of tyrosine to phosphotyrosine participates significantly in pathways such as the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways are important for various cellular processes including growth and survival. Proteins like EGFR and PDGFR regulate signaling cascades by phosphorylation of tyrosine residues and often interact with phosphotyrosine and phosphotyrosine antibodies importantly.

The dysregulation of phosphotyrosine levels contributes to conditions like cancer and diabetes. Aberrant activation or overexpression of phosphotyrosine residues on receptors like HER2 and insulin receptors can promote uncontrolled cell proliferation often seen in tumors and contribute to insulin resistance respectively. As such phosphotyrosine serves not only as a biological target but also as a potential therapeutic target in the treatment of these disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information Phosphotyrosine
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB9319

Anti-Phosphotyrosine antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phosphotyrosine antibody (AB9319)

  • 4
  • 2
Primary Antibodies

AB9331

Biotin Anti-Phosphotyrosine antibody

Western blot - Biotin Anti-Phosphotyrosine antibody (AB9331)

  • 0
  • 0
Primary Antibodies

AB5592

Anti-Phosphotyrosine antibody [IG2]

Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [IG2] (AB5592)

  • 0
  • 0
Primary Antibodies

AB10321

Anti-Phosphotyrosine antibody [PY20]

Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [PY20] (AB10321)

  • 4
  • 6
Primary Antibodies

AB16389

HRP Anti-Phosphotyrosine antibody [PY20]

Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (AB16389)

  • 5
  • 1
Primary Antibodies

AB197508

Alexa Fluor® 647 Anti-Phosphotyrosine antibody [PY20]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Phosphotyrosine antibody [PY20] (AB197508)

  • 0
  • 0
Primary Antibodies

AB9329

HRP Anti-Phosphotyrosine antibody

Western blot - HRP Anti-Phosphotyrosine antibody (AB9329)

  • 4
  • 1
Primary Antibodies

AB190824

Anti-Phosphotyrosine antibody [RM111]

Recombinant

Western blot - Anti-Phosphotyrosine antibody [RM111] (AB190824)

  • 0
  • 0

Complementary Products

Assay Kits

AB126431

Phosphotyrosine In-Cell ELISA Kit

In-Cell ELISA - Phosphotyrosine In-Cell ELISA Kit (AB126431)

  • 0
  • 0
Primary Antibodies

AB205479

Alexa Fluor® 488 Anti-Phosphotyrosine antibody [EPR16871]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Phosphotyrosine antibody [EPR16871] (AB205479)

  • 0
  • 0
Primary Antibodies

AB197508

Alexa Fluor® 647 Anti-Phosphotyrosine antibody [PY20]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Phosphotyrosine antibody [PY20] (AB197508)

  • 0
  • 0
Primary Antibodies

AB9319

Anti-Phosphotyrosine antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phosphotyrosine antibody (AB9319)

  • 4
  • 2
Primary Antibodies

AB16389

HRP Anti-Phosphotyrosine antibody [PY20]

Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (AB16389)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com