JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB311130

Alexa Fluor® 647 Anti-PHOX2B antibody [EPR14423] - C-terminal

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal PHOX2B antibody - conjugated to Alexa Fluor® 647. Cited in 2 publications.

View Alternative Names

PMX2B, PHOX2B, Paired mesoderm homeobox protein 2B, Neuroblastoma Phox, PHOX2B homeodomain protein, Paired-like homeobox 2B, NBPhox

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PHOX2B antibody [EPR14423] - C-terminal

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-PHOX2B antibody [EPR14423] - C-terminal

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-PHOX2B antibody [EPR14423] - C-terminal

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-PHOX2B antibody [EPR14423] - C-terminal

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-PHOX2B antibody [EPR14423] - C-terminal

  • Unconjugated

    Anti-PHOX2B antibody [EPR14423] - C-terminal

  • Carrier free

    Anti-PHOX2B antibody [EPR14423] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14423

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PHOX2B also known as Paired-like Homeobox 2B is a transcription factor that plays a significant role in neural development. It is encoded by the PHOX2B gene and has a molecular weight of approximately 31 kDa. This protein is highly expressed in the central and peripheral nervous systems where it influences the differentiation and function of neural crest-derived cells. PHOX2B is particularly active in the brainstem regions that control vital autonomic functions.
Biological function summary

PHOX2B influences the development of noradrenergic neurons and parasympathetic ganglia. It often functions as part of a transcription factor complex interacting with other proteins to drive the expression of genes required for the autonomic nervous system. The control PHOX2B exerts over neuronal differentiation makes it essential for maintaining the proper functioning of neurons responsible for involuntary bodily processes.

Pathways

PHOX2B is integral to the development and maintenance of the autonomic nervous and respiratory systems. It interacts closely with RET a receptor tyrosine kinase within the neurotrophic signaling pathways that regulate neuronal growth and survival. Additionally the protein plays a role in the endothelin signaling pathway further demonstrating its importance in neurodevelopment.

Alterations in PHOX2B have been linked to disorders such as congenital central hypoventilation syndrome (CCHS) and neuroblastoma. CCHS results from mutations in the PHOX2B gene affecting respiratory control. In the context of cancer PHOX2B neuroblastoma pertains to its potential role in tumor formation alongside MYCN amplification which is a common alteration in neuroblastoma. Understanding PHOX2B’s interactions provides insights into its potential as a target for therapeutic interventions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element.
See full target information PHOX2B
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:3840 PubMed40268933

2025

Neural representation of cytokines by vagal sensory neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Tomás S Huerta,Adrian C Chen,Saher Chaudhry,Aisling Tynan,Timothy Morgan,Kicheon Park,Richard Adamovich-Zeitlin,Bilal Haider,Jian Hua Li,Mitali Nagpal,Stavros Zanos,Valentin A Pavlov,Michael Brines,Theodoros P Zanos,Sangeeta S Chavan,Kevin J Tracey,Eric H Chang

Histology and histopathology 40:711-720 PubMed39363579

2024

CRISPR-mediated WNK4 point mutation aggravates tumor progression and weakens chemotherapy sensitivity in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojun Ying,Zhen Ying,Xiaobing Gao,Yong Wang,Xinting Lv
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB198337

Alexa Fluor® 647 Anti-RAB7 antibody [EPR7589] - Late Endosome Marker

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-RAB7 antibody [EPR7589] - Late Endosome Marker (AB198337)

  • 0
  • 0
Primary Antibodies

AB252131

Alexa Fluor® 647 Anti-Myeloperoxidase antibody [EPR17996]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Myeloperoxidase antibody [EPR17996] (AB252131)

  • 4
  • 1
Primary Antibodies

AB281764

Alexa Fluor® 555 Anti-CD16 antibody [EPR16784]

RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CD16 antibody [EPR16784] (AB281764)

  • 0
  • 0
Primary Antibodies

AB216897

Alexa Fluor® 488 Anti-HNF-4-alpha antibody [EPR3648]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-HNF-4-alpha antibody [EPR3648] (AB216897)

  • 3
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com