Alexa Fluor® 647 Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (AB283820)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with ab283820 at 1/100 (5.0 ug/ml).

Negative control : no staining on human cerebrum.

The section was incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab283820 at 1/100 dilution (shown in Red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (AB283820)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with ab283820 at 1/100 (5.0 ug/ml). Membranous staining on human breast.

The section was incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab283820 at 1/100 dilution (shown in Red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (AB283820)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with ab283820 at 1/100 (5.0 ug/ml). Membranous staining on human breast carcinoma.

The section was incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab283820 at 1/100 dilution (shown in Red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)