JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB203328

Alexa Fluor® 647 Anti-PIAS1 antibody [EPR2580(2)]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal PIAS1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DDXBP1, PIAS1, E3 SUMO-protein ligase PIAS1, DEAD/H box-binding protein 1, E3 SUMO-protein transferase PIAS1, Gu-binding protein, Protein inhibitor of activated STAT protein 1, RNA helicase II-binding protein, GBP

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PIAS1 antibody [EPR2580(2)] (AB203328)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PIAS1 antibody [EPR2580(2)] (AB203328)

ab203328 staining PIAS1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203328 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PIAS1 antibody [EPR2580(2)]

  • 578 PE

    PE Anti-PIAS1 antibody [EPR2580(2)]

  • 660 APC

    APC Anti-PIAS1 antibody [EPR2580(2)]

  • HRP

    HRP Anti-PIAS1 antibody [EPR2580(2)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-PIAS1 antibody [EPR2580(2)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-PIAS1 antibody [EPR2580(2)]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-PIAS1 antibody [EPR2580(2)]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-PIAS1 antibody [EPR2580(2)]

  • Unconjugated

    Anti-PIAS1 antibody [EPR2580(2)]

  • Carrier free

    Anti-PIAS1 antibody [EPR2580(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2580(2)

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min).</p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIAS1 also known as Protein Inhibitor of Activated STAT 1 functions as a E3 SUMO-protein ligase which regulates protein function and localization through sumoylation. It has a molecular mass of approximately 70 kDa. PIAS1 is widely expressed in a variety of tissues indicating its involvement in diverse cellular functions. This protein binds to target proteins and modulates their activity impacting how cells respond to different stimuli.
Biological function summary

PIAS1 influences transcription regulation and gene expression. It acts as a transcriptional co-regulator by interacting with various transcription factors and modulating their activity. PIAS1 is part of multiprotein complexes that mediate these regulatory processes. Its activity is important for maintaining a balanced gene expression which if uncontrolled may result in abnormal cell behavior.

Pathways

PIAS1 plays an important role in signal transduction pathways such as the JAK/STAT pathway and the NF-kB signaling pathway. It interacts with proteins like STAT an important component in these pathways to regulate immune responses and cell growth. PIAS1 modulates the activity of these pathways in part by the covalent attachment of the small ubiquitin-like modifier (SUMO) proteins demonstrating its importance in cellular signaling networks.

PIAS1 relates closely to cancer progression and autoimmune diseases. Abnormal PIAS1 function or expression has links to certain cancers due to its role in controlling cell proliferation and differentiation. In autoimmune disorders PIAS1 affects immune regulation through its interaction with Signal Transducer and Activator of Transcription proteins showing altered activity in diseases like rheumatoid arthritis. Understanding PIAS1's contributions to these pathological states helps in developing targeted therapies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor (PubMed : 11583632, PubMed : 11867732, PubMed : 14500712, PubMed : 21965678, PubMed : 36050397). Catalyzes sumoylation of various proteins, such as CEBPB, MRE11, MTA1, PTK2 and PML (PubMed : 11583632, PubMed : 11867732, PubMed : 14500712, PubMed : 21965678, PubMed : 36050397). Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway (PubMed : 11583632, PubMed : 11867732). In vitro, binds A/T-rich DNA (PubMed : 15133049). The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context (PubMed : 11583632, PubMed : 11867732, PubMed : 14500712, PubMed : 21965678, PubMed : 36050397). Mediates sumoylation of MRE11, stabilizing MRE11 on chromatin during end resection (PubMed : 36050397). Sumoylates PML (at 'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation (By similarity). PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed : 21965678). Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (By similarity). Mediates the nuclear mobility and localization of MSX1 to the nuclear periphery, whereby MSX1 is brought into the proximity of target myoblast differentiation factor genes (By similarity). Also required for the binding of MSX1 to the core enhancer region in target gene promoter regions, independent of its sumoylation activity (By similarity). Capable of binding to the core enhancer region TAAT box in the MYOD1 gene promoter (By similarity).. (Microbial infection) Restricts Epstein-Barr virus (EBV) lytic replication by acting as an inhibitor for transcription factors involved in lytic gene expression (PubMed : 29262325). The virus can use apoptotic caspases to antagonize PIAS1-mediated restriction and express its lytic genes (PubMed : 29262325).
See full target information PIAS1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 21:407 PubMed33853558

2021

Humanized anti-DEspR IgG4 antibody increases overall survival in a pancreatic cancer stem cell-xenograft peritoneal carcinomatosis rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher M Gromisch,Glaiza L A Tan,Khristine Amber Pasion,Ann-Marie Moran,Matthew S Gromisch,Mark W Grinstaff,Francis J Carr,Victoria L M Herrera,Nelson Ruiz-Opazo
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB263970

Anti-PIAS1 antibody

Western blot - Anti-PIAS1 antibody (AB263970)

  • 0
  • 0
Primary Antibodies

AB32219

Anti-PIAS1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PIAS1 antibody (AB32219)

  • 4
  • 6
Primary Antibodies

AB239973

Anti-PIAS1 antibody [EPR2580(2)] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PIAS1 antibody [EPR2580(2)] - BSA and Azide free (AB239973)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB137500

Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody

Immunocytochemistry/ Immunofluorescence - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (AB137500)

  • 0
  • 1
Primary Antibodies

AB194719

Alexa Fluor® 647 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB194719)

  • 5
  • 1
Primary Antibodies

AB209439

PE Anti-VEGFA antibody [EP1176Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - PE Anti-VEGFA antibody [EP1176Y] (AB209439)

  • 0
  • 0
Primary Antibodies

AB204014

HRP Anti-YB1 antibody [EP2708Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-YB1 antibody [EP2708Y] (AB204014)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com