AB246778

Alexa Fluor® 647 Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Prostaglandin E Receptor EP2/PTGER2 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Prostaglandin E2 receptor EP2 subtype, PGE receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostanoid EP2 receptor, PTGER2

2 Images

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (AB246778)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (AB246778)

Flow cytometric analysis of 4% PFA-fixed, 90% methanol-permeabilized JAR cells labeling Prostaglandin E Receptor EP2/PTGER2 with ab246778 at 1/500 dilution (red) compared with ab199093 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Alexa Fluor^® 647) (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue).

Blocking : 10% Goat serum.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (AB246778)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (AB246778)

ab246778 staining Prostaglandin E Receptor EP2 in JAR cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab246778 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).

HRP

HRP Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8030(B)

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "This product gave a positive signal in Jar fixed with 4% formaldehyde (10 min).", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prostaglandin E Receptor EP2 also known as PTGER2 or EP2 receptor is a G-protein coupled receptor with a molecular mass of approximately 53 kDa. This receptor primarily binds prostaglandin E2 (PGE2) mediating various cellular responses. EP2 receptor is expressed in tissues such as the brain kidneys lungs and immune cells. Its activation leads to the stimulation of adenylyl cyclase increasing levels of cyclic AMP (cAMP) within the cell.

Biological function summary

The EP2 receptor plays a role in vasodilation immune modulation and regulation of pain and inflammation. It does not act as part of a large protein complex but rather exerts its effects individually through its coupling with G-proteins. EP2 receptor stimulation results in smooth muscle relaxation and has implications in neuroinflammation and renal function.

Pathways

The EP2 receptor is involved in the prostaglandin signaling pathway and contributes to the inflammatory response pathway. It shares a connection with other receptors such as EP1 EP3 and EP4 which also bind PGE2 but induce different cellular responses. These pathways integrate signals that modulate processes like inflammation and immune responses highlighting EP2's role in maintaining physiological balance.

EP2 receptor activity is associated with conditions like asthma and Alzheimer's disease. In asthma the receptor's activity may influence airway hyperresponsiveness through its effects on smooth muscle and immune cells. In Alzheimer's EP2 receptor-related pathways may contribute to neuroinflammation which is linked with disease progression. Connections with proteins like COX-2 can be noted in these disorders as COX-2-dependent prostaglandin synthesis directly affects EP2 receptor signaling and subsequent cellular outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.

See full target information Prostaglandin E2 receptor EP2 subtype

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:2177-2191 PubMed40858905

2025

ERG-driven prostate cancer initiation is cell-context dependent and requires KMT2A and DOT1L.

Applications

Unspecified application

Species

Unspecified reactive species

Weiran Feng,Erik Ladewig,Matthew Lange,Nazifa Salsabeel,Huiyong Zhao,Young Sun Lee,Anuradha Gopalan,Hanzhi Luo,Wenfei Kang,Ning Fan,Eric Rosiek,Elisa de Stanchina,Yu Chen,Brett S Carver,Christina S Leslie,Charles L Sawyers

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)]

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (AB246778)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (AB246778)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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