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AB237232

Alexa Fluor® 647 Anti-Proteasome 20S LMP2 antibody [EPR13785]

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(1 Publication)

Rabbit Recombinant Monoclonal Proteasome 20S LMP2 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

LMP2, PSMB6i, RING12, PSMB9, Proteasome subunit beta type-9, Low molecular mass protein 2, Macropain chain 7, Multicatalytic endopeptidase complex chain 7, Proteasome chain 7, Proteasome subunit beta-1i, Really interesting new gene 12 protein

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Proteasome 20S LMP2 antibody [EPR13785] (AB237232)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Proteasome 20S LMP2 antibody [EPR13785] (AB237232)

ab237232 staining Proteasome 20S LMP2 in Ramos cells. The cells were fixed with 80% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237232 at 1/250 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Unconjugated

    Anti-Proteasome 20S LMP2 antibody [EPR13785]

  • Carrier free

    Anti-Proteasome 20S LMP2 antibody [EPR13785] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13785

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p>This product gave a positive signal in Ramos fixed with 80% methanol (5 min).</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The proteasome LMP2 also known as proteasome subunit beta type-1 or PSMB9 is a component important in the functioning of the 20S proteasome complex. This molecular entity with a mass of approximately 23 kDa is predominantly expressed in immune cells including lymphocytes. LMP2 incorporates into the immunoproteasome a specialized variant of the proteasome that participates prominently in the processing of class I MHC peptides enhancing the immune response.
Biological function summary

LMP2 is integral to protein catabolism and regulation of the cellular protein homeostasis. As part of the larger proteasome complex the LMP2 protein functions in degradative pathways that recycle amino acids from ubiquitin-tagged proteins. This proteasome takes part in antigen processing helping convert proteins into peptides that are presented on the surface of cells for immune system recognition.

Pathways

LMP2 is significantly involved in the ubiquitin-proteasome pathway and immune system processes. It interacts with other immunoproteasome subunits like LMP7 and MECL-1 which work together to enhance the proteolytic capacity for antigen presentation. The presence of LMP2 in these pathways demonstrates its role in adaptive immunity contributing to the clearance of misfolded or damaged proteins and therefore maintaining cell health.

LMP2 associates with immune-related conditions and some cancer types. Dysregulation of LMP2 expression or function can impact antigen presentation potentially leading to autoimmune disorders due to inappropriate immune activation. Moreover alterations in LMP2 expression have been observed in certain cancers such as melanoma where it affects proteasome activity and influences tumor progression. In these contexts interactions with proteins like TAP1 and TAP2 which aid in peptide transport mark its relevance in disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH (PubMed : 33727065, PubMed : 34819510). The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.
See full target information PSMB9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

British journal of pharmacology 178:2443-2460 PubMed33661551

2021

Tectorigenin alleviates intrahepatic cholestasis by inhibiting hepatic inflammation and bile accumulation via activation of PPARγ.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqing Xiang,Guangyan Yang,Chuanrui Ma,Lingling Wei,Han Wu,Wei Zhang,Xiuhua Tao,Lingyun Jiang,Zhen Liang,Lin Kang,Shu Yang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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