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AB310003

Alexa Fluor® 647 Anti-Protein Phosphatase 1 beta antibody [EP1804Y]

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Rabbit Recombinant Monoclonal PPP1CB antibody - conjugated to Alexa Fluor® 647.

View Alternative Names

Serine/threonine-protein phosphatase PP1-beta catalytic subunit, PP-1B, PPP1CD, PPP1CB

  • Unconjugated

    Anti-PPP1CB antibody [EP1804Y]

  • Carrier free

    Anti-PPP1CB antibody [EP1804Y] - BSA and Azide free

  • 578 PE

    PE Anti-Protein Phosphatase 1 beta antibody [EP1804Y]

  • 660 APC

    APC Anti-Protein Phosphatase 1 beta antibody [EP1804Y]

  • HRP

    HRP Anti-Protein Phosphatase 1 beta antibody [EP1804Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Protein Phosphatase 1 beta antibody [EP1804Y]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-PPP1CB antibody [EP1804Y]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-PPP1CB antibody [EP1804Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Protein Phosphatase 1 beta antibody [EP1804Y]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Protein Phosphatase 1 beta antibody [EP1804Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1804Y

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPP1CB also known as Protein Phosphatase 1 Catalytic Subunit Beta is a member of the catalytic subunit of Protein Phosphatase 1 (PP1). It has an approximate mass of 37 kDa. PPP1CB is expressed in various tissues throughout the human body including the heart brain and skeletal muscle. This phosphatase is primarily involved in the dephosphorylation of proteins a critical function to regulate cellular processes. As a catalytic subunit PPP1CB mediates various signaling cascades by removing phosphate groups from target proteins.
Biological function summary

PPP1CB plays key roles in cell division signal transduction and muscle contraction. It acts as part of a larger complex interacting with a variety of regulatory proteins and substrates to fine-tune cellular activities. Through dephosphorylation PPP1CB aids in the transition between different phases of cell cycles impacting cellular growth and proliferation. It is also involved in glycogen metabolism where it removes phosphate groups from glycogen phosphorylase ensuring proper energy balance in cells.

Pathways

PPP1CB participates significantly in the TGF-beta signaling and Wnt signaling pathways. It interacts with several proteins such as SMADs in the TGF-beta pathway contributing to the regulation of transcription and cellular responses to growth factors. In the Wnt signaling pathway PPP1CB deactivates key kinase members thereby modulating cell fate decisions and embryonic development. These pathways underline the phosphate turnover that PPP1CB controls impacting various cellular functions.

PPP1CB has been associated with cancer development and cardiovascular diseases. It contributes to oncogenesis when its regulatory role in cell cycle controls is disrupted leading to unchecked cell proliferation. Alterations in PPP1CB activity have also been linked to heart diseases where improper dephosphorylation affects muscle contraction and cardiac function. In cancer interactions with proteins such as PP2A alter the signaling balance while in heart disease proteins like PLN may connect to aberrant PPP1CB function emphasizing its critical role in maintaining cellular health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed : 23396208). Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation (PubMed : 35768504, PubMed : 35831509, PubMed : 36175670). The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities (PubMed : 35768504, PubMed : 35831509, PubMed : 36175670). The SMP complex enhances the dephosphorylation activity and substrate specificity of PP1c (PubMed : 35768504, PubMed : 36175670).
See full target information PPP1CB
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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