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AB323839

Alexa Fluor® 647 Anti-PTPN12 antibody [EPR28198-148]

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Rabbit Recombinant Monoclonal PTPN12 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Tyrosine-protein phosphatase non-receptor type 12, PTP-PEST, Protein-tyrosine phosphatase G1, PTPG1, PTPN12

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-PTPN12 antibody [EPR28198-148] (AB323839)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-PTPN12 antibody [EPR28198-148] (AB323839)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling PTPN12 with ab323839 at 1/50 (10.0 ug/ml) dilution.

Positive staining on human breast cancer.
The primary antibody was incubated overnight at 4°C (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-PTPN12 antibody [EPR28198-148] (AB323839)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-PTPN12 antibody [EPR28198-148] (AB323839)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PTPN12 with ab323839 at 1/50 (10.0 ug/ml) dilution.

Positive staining on human colon.
The primary antibody was incubated overnight at 4°C (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28198-148

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Human

Applications

Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB314375

Human PTPN12 ELISA Kit

Sandwich ELISA - Human PTPN12 ELISA Kit (AB314375)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Protein Tyrosine Phosphatase Non-Receptor Type 12 (PTPN12) often referred to as PTP-PeST is an enzyme that plays a significant mechanical role within cells. PTPN12 is part of the protein tyrosine phosphatase (PTP) family which is responsible for removing phosphate groups from phosphotyrosine residues on proteins. This activity regulates many cellular processes including growth and differentiation. PTPN12 has a molecular mass of approximately 86 kDa. It is ubiquitously expressed in various tissues but shows higher expression in hematopoietic cells.
Biological function summary

As a non-receptor type phosphatase PTPN12 partners with several proteins to perform essential cellular functions. It interacts with multiple components in the cell participating in the assembly of protein complexes that manage cytoskeletal dynamics and cell movement. PTPN12 influences signal transduction pathways by interacting with adapter proteins such as p130Cas and Paxillin which are vital for cell adhesion and migration. It also plays a role in maintaining cellular homeostasis by controlling signaling cascades that prevent excessive cell proliferation.

Pathways

PTPN12 takes part in critical signal transduction networks most notably the Integrin signaling and RhoA pathways. In the Integrin pathway PTPN12 negatively regulates signaling by dephosphorylating focal adhesion-associated proteins. This regulation affects cell attachment to the extracellular matrix and cell motility. The interaction of PTPN12 with proteins such as Src and FAK (Focal Adhesion Kinase) illustrates its involvement in these processes. In the RhoA pathway PTPN12 influences cytoskeletal rearrangement and cell shape regulation linking to its role in cell migration.

Disruptions in PTPN12 function associate with cancer progression such as breast cancer and inflammatory disorders. In breast cancer physiological studies indicate that PTPN12 acts as a tumor suppressor. Its loss or reduced activity may lead to unregulated cell proliferation and metastasis by failing to restrain pathways involving Src and FAK. In inflammatory disorders altered activities of PTPN12 can affect immune cell function contributing to conditions like rheumatoid arthritis. The enzyme's dysfunction can amplify inflammatory signals through faults in the regulation of immune cells linking PTPN12 to increased disease susceptibility.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dephosphorylates a range of proteins, and thereby regulates cellular signaling cascades (PubMed : 18559503). Dephosphorylates cellular tyrosine kinases, such as ERBB2 and PTK2B/PYK2, and thereby regulates signaling via ERBB2 and PTK2B/PYK2 (PubMed : 17329398, PubMed : 27134172). Selectively dephosphorylates ERBB2 phosphorylated at 'Tyr-1112', 'Tyr-1196', and/or 'Tyr-1248' (PubMed : 27134172).
See full target information PTPN12
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com