Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control Suitable for ICC/IF, Flow Cyt and reacts with samples. Cited in 19 publications.
IgG
Rabbit
Alexa Fluor® 647
Ex: 650nm, Em: 665nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application ICC/IF | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used. |
Select an associated product type
Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control Suitable for ICC/IF, Flow Cyt and reacts with samples. Cited in 19 publications.
IgG
Rabbit
Alexa Fluor® 647
Ex: 650nm, Em: 665nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
EPR25A
Affinity purification Protein A
Please note Abcam have optimised the validation of this product. In our hands, we observe an increase in background signal intensity with the use of Triton X-100 and would recommend using an alternative permeabilisation method such as methanol or saponin.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Store in the dark
KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with ab199093 using various fixation and permeablisation. The cells were fixed, washed and permeablised as indicated below;
4% formaldehyde (10 min, room temperature)/0.1% PBS-Triton X-100 (15 min, room temperature)
80% methanol (5 min, -20°C)/0.1% PBS-Triton X-100 (15 min, room temperature)
4% formaldehyde (10 min, room temperature)/90% methanol (30 min, -20°C)
The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091) for 30 min at 22°C at the following concentrations - Blue line (Unlabelled), Black line (0.01μg/ml), Red line (0.1μg/ml) and Green line (1μg/ml) .
Acquisition of >5,000 events were collected using a 40mW Red laser (640nm) and 670/14 bandpass filter.
Immunofluorescent analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, fixed with 4% formaldehyde (10 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199093 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/500 dilution (showing no signal) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).
Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.
The initial sample was stained using an antibody cocktail and anti-EGFR antibody. A further slide was fixed with 4% formaldehyde for 10 mins, permeabilized with 0.2% Triton X-100 for 5 mins and blocked with 10% fetal-bovine serum in 0.1% PBS-Tween for 1 h at room temperature. The cells were incubated overnight at 4 °C with anti-EGFR antibody and anti-PD-L1 antibody [28-8] (Alexa Fluor® 647) (Abcam Alexa Fluor® 647 Anti-PD-L1 antibody [28-8] - Extracellular domain ab209960) 1/100 dilution. Nuclear DNA was visualized with DAPI. Rabbit IgG monoclonal isotype control (Alexa Fluor®647) (ab199093) was used to identify nonspecific binding. Cells were imaged on the Olympus IX3 inverted microscope.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) treated with 1uM Puromycin for 30min (Green) / Untreated HeLa (Magenta) cells labelling Puromycin with Alexa Fluor® 647 Anti-Puromycin antibody [EPR27218-173] ab322729 at 1/500 dilution (0.1ug) (Magenta) and (Green) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) and (Grey).
Flow cytometric analysis of Mouse splenocytes cells labelling CD23 with Alexa Fluor® 647 Anti-CD23 antibody [EPR28712-26] ab322424 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Gated on viable cells.
Cells are co-stained with CD19 conjugated to PE/CY7.
Flow cytometric analysis of Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Left) / HeLa (human cervical adenocarcinoma epithelial cell, Right) cells labelling LTBR with Alexa Fluor® 647 Anti-LTBR antibody [EPR26097-53] ab318170 at 1/5000 dilution(0.01ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Gated on viable cells.
Negative control: Jurkat (PMID: 10200501).
Flow cytometric analysis of Mouse splenocytes cells labelling CD23 with Alexa Fluor® 647 Anti-CD23 antibody [EPR28712-26] ab322424 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Gated on viable cells.
Cells are co-stained with CD3 conjugated to Alexa Fluor®488.
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling Dectin-1 with Alexa Fluor® 647 Anti-Dectin-1 antibody [EPR28032-27] ab318175 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Gated on viable cells.
Cells are co-stained with CD3 conjugated to Alexa Fluor® 488.
Flow cytometric analysis of A549 (human lung carcinoma epithelial cell, Left) / HUVEC (human umbilical vein endothelial cell, Right) cells labelling DARC with Alexa Fluor® 647 Anti-DARC antibody [EPR26544-110] ab318172 at 1/5000 dilution(0.01ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Gated on viable cells.
Negative control: A549.
Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype control (Left) / Human peripheral blood mononuclear cell (PBMC) treated with 50ng/ml PMA, 250ng/ml ionomycin and 300ng/ml Brefeldin A for 16hours (Middle) / Untreated control (Right) cells labelling TNF alpha with Alexa Fluor® 647 Anti-TNF alpha antibody [EPR22598-212] ab318238 at 1/500 dilution (0.1ug) / Middle and Right (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Cells were stained with anti-CD3 conjugated to Alexa Fluor® 488. Then fixed with 2% PFA for 10min followed by intracellularly stained with rabbit IgG or Alexa Fluor® 647 Anti-TNF alpha antibody [EPR22598-212] ab318238
Flow cytometric analysis of Isotype (Left) / Human peripheral blood mononuclear cell (PBMC) treated with 100ng/ml GM-CSF and 50ng/ml IL-4 for 6 days (Middle) / Untreated control (Right) cells labelling CMKLR1 with Alexa Fluor® 647 Anti-CMKLR1 antibody [EPR26501-70] ab318242 at 1/500 dilution (0.1ug)/ Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Gated on viable cells.
Cells were stained with rabbit IgG or Alexa Fluor® 647 Anti-CMKLR1 antibody [EPR26501-70] ab318242. Then stained with anti-CD123 conjugated to FITC.
Flow cytometric analysis of Isotype (Left) / Human peripheral blood mononuclear cell (PBMC) treated with 100ng/ml GM-CSF and 50ng/ml IL-4 for 6 days (Middle) / Untreated control (Right) cells labelling CMKLR1 with Alexa Fluor® 647 Anti-CMKLR1 antibody [EPR26501-70] ab318242 at 1/500 dilution (0.1ug)/ Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Gated on viable cells.
Cells were stained with rabbit IgG or Alexa Fluor® 647 Anti-CMKLR1 antibody [EPR26501-70] ab318242. Then stained with anti-HLA-DR conjugated to PE.
Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / 293T cells transfected with a human EMR1/ADGRE1expression vector containing a Myc tag (Middle) / 293T cells transfected with an empty vector containing a Myc tag (Right) cells labelling EMR1/ADGRE1 with Alexa Fluor® 647 Anti-EMR1/ADGRE1 antibody [EPR23225-94] ab322230 at 1/500 dilution (0.1ug) / Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Live cells were surface stained with ab, then fixed with 2% PFA followed by intracellular staining with Myc-tag conjugated to Alexa Fluor® 488.
Flow cytometric analysis of B16-F10 (mouse skin melanoma cell, Left) / bEnd.3 (mouse brain endothelial cell, Right) cells labelling EPCR/CD201 with Alexa Fluor® 647 Anti-EPCR/CD201 antibody [EPR26267-19] ab322227 at 1/50000 dilution (0.001ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Gated on viable cells.
Low expression: B16-F10 (PMID: 19188668).
Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CCR6 with Alexa Fluor® 647 Anti-CCR6 antibody [EPR24590-123] ab322226 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Cells were co-stained with anti-CD19 conjugated to PE-Cy7.
Gated on viable cells.
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD2 with Alexa Fluor® 647 Anti-CD2 antibody [EPR27426-17] ab320120 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Cells are co-stained with CD14 conjugated to FITC.
Gated on viable cells.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Transaldolase 1 with Alexa Fluor® 647 Anti-Transaldolase 1 antibody [EPR28140-51] ab322241 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD21 with Alexa Fluor® 647 Anti-CD21 antibody [EPR27369-9] ab320784 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Gated on viable cells.
Cells are co-stained with CD56 conjugated to Brilliant Violet 421.
Flow cytometric analysis of EL4 (mouse lymphoma T lymphocyte, Left) / RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells labelling CLEC5A with Alexa Fluor® 647 Anti-CLEC5A antibody [EPR28104-14] ab321947 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Negative control: EL4.
Gated on viable cells.
Flow cytometric analysis of Parental Jurkat (Left) / CD1A KO Jurkat (CD1A knockout human T cell leukemia T lymphocyte from peripheral blood, Right) cells labelling CD1a with Alexa Fluor® 647 Anti-CD1a antibody [EPR26526-62] ab321943 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Gated on viable cells.
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD21 with Alexa Fluor® 647 Anti-CD21 antibody [EPR27369-9] ab320784 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Gated on viable cells.
Cells are co-stained with CD19 conjugated to PE/CY7.
Flow cytometric analysis of K-562 (human chronic myelogenous leukemia lymphoblast, Left) / Raji (human Burkitt's lymphoma B lymphocyte, Right) cells labelling CD21 with Alexa Fluor® 647 Anti-CD21 antibody [EPR27369-9] ab320784 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Negative control: K-562 (PMID: 6983911).
Gated on viable cells.
Flow cytometric analysis of Daudi (human Burkitt's lymphoma lymphoblast, Left) / KG-1a (human bone marrow myeloblast, Right) cells labelling CD34 with Alexa Fluor® 647 Anti-CD34 antibody [EPR27432-54] ab321945 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Negative control: Daudi (PMID: 21286385, 10942240).
Gated on viable cells.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized AtT-20 (mouse pituitary tumor cell, Left) / Hepa1-6 (mouse hepatoma epithelial cell, Right) cells labelling PCSK9 with Alexa Fluor® 647 Anti-PCSK9 antibody [EPR17827-117] ab322020 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Low expression: AtT-20 (PMID: 28971102).
Flow cytometric analysis of EL4 (mouse lymphoma T lymphocyte, Left) / NIH/3T3 (mouse embryonic fibroblast, Right) cells labelling OSMR with Alexa Fluor® 647 Anti-OSMR antibody [EPR28222-64] ab322133 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Negative control: EL4.
Gated on viable cells.
Flow cytometric analysis of Mouse splenocytes cells labelling CLEC5A with Alexa Fluor® 647 Anti-CLEC5A antibody [EPR28104-14] ab321947 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Cells are co-stained with CD3 conjugated to Alexa Fluor®488.
Gated on viable cells.
Flow cytometric analysis of Mouse splenocytes cells labelling CLEC5A with Alexa Fluor® 647 Anti-CLEC5A antibody [EPR28104-14] ab321947 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Cells are co-stained with CD11b conjugated to Brilliant Violet 421.
Gated on viable cells.
Flow cytometric analysis of M1 (mouse myeloid leukemia myeloblast, Left) / 3T3-L1 (mouse embryonic fibroblast, Right) cells labelling OSMR with Alexa Fluor® 647 Anti-OSMR antibody [EPR28222-64] ab322133 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Negative control: M1.
Gated on viable cells.
Flow cytometric analysis of HeLa (human cervical adenocarcinoma epithelial cell, Left) / MOLT-4 (human lymphoblastic leukemia T lymphoblast, Right) cells labelling CD1a with Alexa Fluor® 647 Anti-CD1a antibody [EPR26526-62] ab321943 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Negative control: HeLa.
Gated on viable cells.
Flow cytometric analysis of Raji (human Burkitt's lymphoma B lymphocyte, Left) / Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Right) cells labelling CD2 with Alexa Fluor® 647 Anti-CD2 antibody [EPR27426-17] ab320120 at 1/500 dilution (0.1ug) (Magenta) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Negative control: Raji (PMID: 32808354).
Gated on viable cells.
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CCR9 with Alexa Fluor® 647 Anti-CCR9 antibody [EPR26524-59] ab320780 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Cells are co-stained with CD56 conjugated to Brilliant Violet 421.
Gated on viable cells.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling Transaldolase 1 with Alexa Fluor® 647 Anti-Transaldolase 1 antibody [EPR28140-51] ab322241 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Flow cytometric analysis of Neuro-2a (mouse neuroblastoma neuroblast, Left) / Raw264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells labelling CD204 with Alexa Fluor® 647 Anti-CD204 antibody [EPR28270-88] ab320786 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Negative control: Neuro-2a.
Gated on viable cells.
Flow cytometric analysis of Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Left) / MOLT-4 (human lymphoblastic leukemia T lymphoblast, Right) cells labelling CCR9 with Alexa Fluor® 647 Anti-CCR9 antibody [EPR26524-59] ab320780 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Negative control: Jurkat (PMID: 19525985).
Gated on viable cells.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (human epidermoid carcinoma epithelial cell, Left) / U-937 (human histiocytic lymphoma monocyte, Right) cells labelling WASP/Wiskott-Aldrich syndrome protein with Alexa Fluor® 647 Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] ab320116 at 1/5000 dilution (0.01ug) (Magenta) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Negative control: A431 (PMID: 9207440).
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD2 with Alexa Fluor® 647 Anti-CD2 antibody [EPR27426-17] ab320120 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Cells are co-stained with CD14 conjugated to Brilliant Violet 421
Gated on viable cells.
Flow cytometric analysis of Isotype (Left) / HEK-293T (human embryonic kidney epithelial cell) transfected with Myc-tagged ENPP3 overexpression construct (Middle) / 293T transfected with an empty vector containing a myc-His tag (Right) cells labelling ENPP3/B10 with Alexa Fluor® 647 Anti-ENPP3/B10 antibody [EPR27349-72] ab319126 at 1/500 dilution (0.1ug) / Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).
Cells were co-stained with anti-myc conjugated to Alexa Fluor 488.
Gated on viable cells.
Flow cytometric analysis of 293T (human embryonic kidney epithelial cell, Left) / KU812 (human Peripheral blood basophil, Right) cells labelling ENPP3/B10 with Alexa Fluor® 647 Anti-ENPP3/B10 antibody [EPR27349-72] ab319126 at 1/500 dilution (0.1ug)compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).
Negative control: 293T (PMID: 11342463).
Gated on viable cells.
Flow cytometric analysis of Isotype (Left) / Human PBMC (human peripheral blood mononuclear cell) treated with 200 ng/ml IL-12 for 6 days (Right) cells labelling IL-18R1 with Alexa Fluor® 647 Anti-IL-18R1 antibody [EPR26127-35] ab319088 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody) (Blue).
Cells co-stained with CD3 conjugated to BV421.
Gated on viable cells.
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling Dectin-1 with Alexa Fluor® 647 Anti-Dectin-1 antibody [EPR28032-27] ab318175 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Gated on viable cells.
Cells are co-stained with CD14 conjugated to Alexa Fluor® 488.
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