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AB225339

Alexa Fluor® 647 Anti-Renilla Luciferase antibody [EPR17792]

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(1 Publication)

Rabbit Recombinant Monoclonal LUCI antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Coelenterazine h 2-monooxygenase, Renilla-luciferin 2-monooxygenase, Renilla-type luciferase

2 Images
Flow Cytometry - Alexa Fluor® 647 Anti-Renilla Luciferase antibody [EPR17792] (AB225339)
  • Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 647 Anti-Renilla Luciferase antibody [EPR17792] (AB225339)

Flow cytometric analysis of 293T transfected with RFP tagged Renilla Luciferase stained with ab225339 (right panel). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225339, 1/500 dilution) for 30 min at 22°C.

Isotype control antibody (left panel) was Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Co-staining with RFP (y-axis).

Acquisition of >5,000 events were collected using a 17 mW red Helium-Neon laser (633nm) and 660/20 bandpass filter.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Renilla Luciferase antibody [EPR17792] (AB225339)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Renilla Luciferase antibody [EPR17792] (AB225339)

ab225339 staining Renilla Luciferase in 293T cells transfected with Renilla Luciferase and normal 293T cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225339 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 578 PE

    PE Anti-Renilla Luciferase antibody [EPR17792]

  • 660 APC

    APC Anti-Renilla Luciferase antibody [EPR17792]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Renilla Luciferase antibody [EPR17792]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Renilla Luciferase antibody [EPR17792]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Renilla Luciferase antibody [EPR17792]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17792

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in 293T cells transfected with Renilla Luciferase fixed with 4% formaldehyde (10 min)</p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in 293T cells transfected with Renilla Luciferase fixed with 4% formaldehyde (10 min)</p>" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in 293T cells transfected with Renilla Luciferase fixed with 4% formaldehyde (10 min)</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Renilla Luciferase also known as RLuc is an enzyme that catalyzes the oxidation of its substrate coelenterazine producing bioluminescence. With a molecular mass of approximately 36 kDa RLuc facilitates this reaction by oxygenation resulting in the emission of blue light. Renilla Luciferase is typically isolated from the sea pansy Renilla reniformis and has been widely used as a reporter gene in molecular biology for monitoring gene expression and cellular events due to its efficient light production.
Biological function summary

Renilla Luciferase operates as part of the bioluminescent system found in certain marine organisms. In its native environment it interacts with other proteins like Green Fluorescent Protein (GFP) to which it transfers energy converting blue light to green fluorescence. This process of bioluminescence serves various ecological functions including predator avoidance and communication between organisms. These interactions highlight the importance of Renilla Luciferase in understanding biological luminescence systems.

Pathways

Researchers use Renilla Luciferase extensively in signaling and metabolic pathway analyses. It plays an important role in the study of G-protein coupled receptor (GPCR) pathways where it can help to analyze receptor activation and downstream communication. In these pathways it often pairs with other luminescent or fluorescent markers like Firefly Luciferase or GFP enabling simultaneous dual-reporter assays to provide more comprehensive data on cellular responses.

Renilla Luciferase aids in the study of cancer and infectious diseases. Its role in reporter assays facilitates the investigation of gene expression changes and the screening of potential therapeutic compounds. For example in cancer research it is frequently paired with p53 proteins for studying apoptosis and cell cycle regulation. Similarly in infectious disease studies Renilla Luciferase reporters can track viral infections and the efficacy of antiviral drugs. These applications highlight its value in advancing biomedical research and drug development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO(2) molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.
See full target information Coelenterazine h 2-monooxygenase
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature medicine 30:3196-3208 PubMed39304781

2024

High-throughput identification of repurposable neuroactive drugs with potent anti-glioblastoma activity.

Applications

Unspecified application

Species

Unspecified reactive species

Sohyon Lee,Tobias Weiss,Marcel Bühler,Julien Mena,Zuzanna Lottenbach,Rebekka Wegmann,Miaomiao Sun,Michel Bihl,Bartłomiej Augustynek,Sven P Baumann,Sandra Goetze,Audrey van Drogen,Patrick G A Pedrioli,David Penton,Yasmin Festl,Alicia Buck,Daniel Kirschenbaum,Anna M Zeitlberger,Marian C Neidert,Flavio Vasella,Elisabeth J Rushing,Bernd Wollscheid,Matthias A Hediger,Michael Weller,Berend Snijder
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com