AB309983

Alexa Fluor® 647 Anti-RYBP antibody [EPR13059(2)]

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal RYBP antibody - conjugated to Alexa Fluor® 647.

View Alternative Names

DEDAF, YEAF1, RYBP, RING1 and YY1-binding protein, Apoptin-associating protein 1, Death effector domain-associated factor, YY1 and E4TF1-associated factor 1, APAP-1, DED-associated factor

578 PE

PE Anti-RYBP antibody [EPR13059(2)]
660 APC

APC Anti-RYBP antibody [EPR13059(2)]
HRP

HRP Anti-RYBP antibody [EPR13059(2)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-RYBP antibody [EPR13059(2)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-RYBP antibody [EPR13059(2)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-RYBP antibody [EPR13059(2)]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-RYBP antibody [EPR13059(2)]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-RYBP antibody [EPR13059(2)] - ChIP Grade
Unconjugated

Anti-RYBP antibody [EPR13059(2)] - ChIP Grade
Carrier free

Anti-RYBP antibody [EPR13059(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13059(2)

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RYBP also known as Ring1 and YY1 Binding Protein has a critical mechanical role in gene regulation. It has a molecular weight of approximately 28 kDa. It is a part of the Polycomb group (PcG) proteins which take part in chromatin remodeling to regulate gene expression. RYBP is found in the nucleus of cells and is known to bind DNA directly. Its expression occurs widely across various tissues indicating its broad involvement in cellular functions.

Biological function summary

This protein actively participates in maintaining transcriptional repression through its involvement in the Polycomb Repressive Complex 1 (PRC1). PRC1 includes other key proteins such as Ring1A/B and CBX proteins which work together to modulate chromatin structure. RYBP contributes to the ubiquitination of histone H2A a process essential for maintaining gene silencing. This activity is important during developmental stages influencing cell fate decisions and lineage commitment.

Pathways

RYBP plays an essential role in the regulation of the Wnt signaling pathway and the apoptosis pathway. Within the Wnt pathway RYBP acts in part by interacting with proteins such as β-catenin influencing cell proliferation and differentiation. In the apoptosis pathway it is involved through its interactions with E3 ubiquitin ligases potentially affecting cell death regulation. These pathways showcase how RYBP integrates into important cellular processes impacting cellular homeostasis.

RYBP has connections to cancer and neurodevelopmental disorders. Its altered expression is linked to tumor progression where it interacts with other PcG proteins like EZH2 often showing overexpression in cancers. These interactions can lead to abnormal gene silencing contributing to oncogenesis. In neurodevelopmental contexts disruptions in RYBP's function are associated with changes in neural differentiation and might correlate with disorders like autism spectrum disorders highlighting its broader relevance beyond typical gene expression roles.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1-like complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed : 25519132). Component of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females. May stimulate ubiquitination of histone H2A 'Lys-119' by recruiting the complex to target sites (By similarity). Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes (PubMed : 19098711). May also regulate the ubiquitin-mediated proteasomal degradation of other proteins like FANK1 to regulate apoptosis (PubMed : 14765135, PubMed : 27060496). May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1 (PubMed : 11953439). May bind to DNA (By similarity). May play a role in the repression of tumor growth and metastasis in breast cancer by down-regulating SRRM3 (PubMed : 27748911).

See full target information RYBP

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-RYBP antibody [EPR13059(2)]

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Immunogen

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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