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AB204028

Alexa Fluor® 647 Anti-S100 alpha 6/PRA antibody [EPNCIR121]

5

(1 Review)

|

(3 Publications)

Rabbit Recombinant Monoclonal S100 alpha 6/PRA antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

CACY, S100A6, Protein S100-A6, Calcyclin, Growth factor-inducible protein 2A9, MLN 4, Prolactin receptor-associated protein, S100 calcium-binding protein A6, PRA

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 alpha 6/PRA antibody [EPNCIR121] (AB204028)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 alpha 6/PRA antibody [EPNCIR121] (AB204028)

ab204028 staining S100 alpha 6/PRA in SKNSH cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204028 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPNCIR121

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB244301

Anti-S100 alpha 6/PRA antibody

Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody (AB244301)

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Primary Antibodies

AB181974

Anti-S100 alpha 6/PRA antibody [EPR13084]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody [EPR13084] (AB181974)

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Primary Antibodies

AB181975

Anti-S100 alpha 6/PRA antibody [EPR13084-69]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (AB181975)

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  • 1

Complementary Products

Assay Kits

AB317542

Human S100 alpha 6/PRA ELISA Kit

SimpleStep

Sandwich ELISA - Human S100 alpha 6/PRA ELISA Kit (AB317542)

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Primary Antibodies

AB196175

Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB196175)

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Primary Antibodies

AB7800

Anti-Cytokeratin 14 antibody [LL002]

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [LL002] (AB7800)

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Primary Antibodies

AB202755

Alexa Fluor® 647 Anti-DCAMKL1 antibody [EPR6085]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DCAMKL1 antibody [EPR6085] (AB202755)

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Assay Kits

AB112156

Rhod-4 Calcium Assay kit - Medium Removal No Wash

Functional Studies - Rhod-4 Calcium Assay kit - Medium Removal No Wash (AB112156)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S100 alpha 6 also known as PRA is a member of the S100 protein family characterized by its small molecular weight of approximately 10 kDa. This protein expresses in various tissues including the heart skeletal muscle kidney and brain. The S100 family proteins commonly contain two EF-hand calcium-binding motifs which play key roles in intracellular and extracellular regulatory activities. S100 alpha 6 participates in calcium-dependent signal transduction processes that affect a range of cellular functions.
Biological function summary

S100 alpha 6 plays a significant role in intracellular calcium signaling and cytoskeletal dynamics. This protein often interacts as a part of protein complexes with various other proteins including annexin and tropomyosin contributing to vital cellular functions like contraction motility and cell cycle regulation. Its expression levels and spatial distribution influence cellular proliferation and differentiation making it an important component in several cell types.

Pathways

The protein S100 alpha 6 participates in essential pathways such as the MAPK and calcium signaling pathways. In the MAPK pathway S100 alpha 6 interacts with proteins like ERK1/2 influencing cell growth and apoptosis signaling cascades. In calcium signaling it helps regulate the release and uptake of calcium ions vital for maintaining cellular homeostasis and function. Through these pathways S100 alpha 6 is interconnected with other proteins like calmodulin and calcyclin which share similar physiological roles.

Increased or decreased expression of S100 alpha 6 relates to conditions such as cancer and cardiomyopathy. In cancer S100 alpha 6 often associates with dysregulated cell growth and can influence tumor progression through its interactions with proteins such as p53 and MDM2. In cardiomyopathy it affects calcium-handling properties in heart muscle cells linking it to cardiac dysfunction. These associations highlight its impact on disease development and progression making it a target for research in therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.. (Microbial infection) Facilitates attachment and invasion of Toxoplasma gondii parasites.
See full target information S100A6
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature 647:517-527 PubMed40866703

2025

Mechanical confinement governs phenotypic plasticity in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Miranda V Hunter,Eshita Joshi,Sydney Bowker,Emily Montal,Yilun Ma,Young Hun Kim,Zhifan Yang,Laura Tuffery,Zhuoning Li,Eric Rosiek,Alexander Browning,Reuben Moncada,Itai Yanai,Helen Byrne,Mara Monetti,Elisa de Stanchina,Pierre-Jacques Hamard,Richard P Koche,Richard M White

Ultrasonics sonochemistry 117:107339 PubMed40215791

2025

Anti-inflammatory and gut microbiota regulatory effects of ultrasonic degraded polysaccharides from Auricularia auricula-judae in DSS-induced colitis mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tahidul Islam,Baojun Xu,Zhaoxiang Bian

Cancer discovery 13:2270-2291 PubMed37553760

2023

GABA Regulates Electrical Activity and Tumor Initiation in Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mohita Tagore,Emiliano Hergenreder,Sarah C Perlee,Nelly M Cruz,Laura Menocal,Shruthy Suresh,Eric Chan,Maayan Baron,Stephanie Melendez,Asim Dave,Walid K Chatila,Jeremie Nsengimana,Richard P Koche,Travis J Hollmann,Trey Ideker,Lorenz Studer,Andrea Schietinger,Richard M White
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com