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AB196175

Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker

4

(1 Review)

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(6 Publications)

Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker - Alexa Fluor® 647 conjugated (ab196175) is a rabbit recombinant monoclonal antibody detecting S100 beta in ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB196175)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB196175)

ab196175 staining S100 beta in A375 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196175 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB196175)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB196175)

ab196175 staining S100 beta in Malme-3M cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196175 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Unconjugated

    Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker

  • Carrier free

    Anti-S100 beta antibody [EP1576Y] - BSA and Azide free

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker

  • HRP

    HRP Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1576Y

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p>This product gave a positive signal in A375 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Goat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Macaque monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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AB314846

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab196175) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone EP1576Y is also available pre-conjugated to a variety of labels for your convenience – Anti-S100 beta Alexa Fluor® 647 [EP1576Y] - Astrocyte Marker (ab196175).

Other related products
We have a range of other formats of antibody clone [EP1576Y] also available for your convenience: ab52642, HRP - ab195535, Alexa Fluor® 647 - ab196175, Carrier free - ab215989, Alexa Fluor® 594 - ab307851, Alexa Fluor® 488 - ab310990, Alexa Fluor® 555 - ab313219, Alexa Fluor® 568 - ab313817

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S100 beta also known as protein S100 is part of the S100 family of proteins characterized by their calcium-binding properties. The S100 beta protein has an approximate molecular mass of 10.7 kDa. This protein is mainly expressed in astrocytes a type of glial cell in the central nervous system. It acts as a marker for astrocytes and is prominently used in astrocyte immunohistochemistry. Additionally it serves as a valuable marker for identifying and studying astrocyte markers because it can highlight the presence of these cells in various contexts.
Biological function summary

The S100 beta protein participates in several intracellular and extracellular activities influencing cell proliferation and differentiation. It forms dimers and sometimes assembles into larger complexes mediated by the binding of calcium ions which alters its functional state. Within the nervous system S100 beta modulates the dynamics between neurons and glial cells affecting processes such as cell survival and neurite outgrowth. This dual role in both supporting and inhibiting various cellular actions highlights its importance in maintaining neural homeostasis.

Pathways

S100 beta engages in signaling pathways related to inflammation and stress responses. It works within the MAPK and NF-kB pathways playing roles in cellular responses to oxidative stress. These pathways involve interactions with other proteins like p53 which S100 beta can modulate to influence apoptosis. The regulatory properties of S100 beta in these pathways link it with broader networks affecting cell fate decisions under pathological and physiological conditions.

S100 beta is associated with neurodegenerative diseases and cancer. Elevated levels of S100 beta are often found in Alzheimer's disease where it interacts with proteins like amyloid-beta contributing to neuroinflammation and neurotoxicity. In cancer particularly malignant melanoma S100 beta levels serve as a biomarker for tumor progression. Its interactions with p53 and other regulatory proteins highlight its involvement in oncogenic processes emphasizing its potential as a target for therapeutic intervention in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain (PubMed : 20950652, PubMed : 6487634). Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer (PubMed : 20950652, PubMed : 6487634). Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites (By similarity). Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation (By similarity). Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue (By similarity). Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase (By similarity). Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization (PubMed : 20351179). May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (PubMed : 22399290).
See full target information S100B
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

JACC. Clinical electrophysiology 8:1191-1215 PubMed36182566

2022

The Heart's Pacemaker Mimics Brain Cytoarchitecture and Function: Novel Interstitial Cells Expose Complexity of the SAN.

Applications

Unspecified application

Species

Unspecified reactive species

Rostislav Bychkov,Magdalena Juhaszova,Miguel Calvo-Rubio Barrera,Lorenzo A H Donald,Christopher Coletta,Chad Shumaker,Kayla Moorman,Syevda Tagirova Sirenko,Alexander V Maltsev,Steven J Sollott,Edward G Lakatta

STAR protocols 3:101109 PubMed35106499

2022

A clearing-free protocol for imaging intact whole adipose tissue innervation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jake W Willows,Magdalena Blaszkiewicz,Kristy L Townsend

iScience 24:103127 PubMed34622172

2021

Visualization and analysis of whole depot adipose tissue neural innervation.

Applications

Unspecified application

Species

Unspecified reactive species

Jake W Willows,Magdalena Blaszkiewicz,Amy Lamore,Samuel Borer,Amanda L Dubois,Emma Garner,William P Breeding,Karissa B Tilbury,Andre Khalil,Kristy L Townsend

Scientific reports 11:16175 PubMed34376743

2021

Increased mortality and altered local immune response in secondary peritonitis after previous visceral operations in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jonas Menz,Laura Hundt,Tobias Schulze,Katrin Schmoeckel,Pia Menges,Grazyna Domanska

Frontiers in cell and developmental biology 8:549353 PubMed33042990

2020

The Spindle-Associated Microcephaly Protein, WDR62, Is Required for Neurogenesis and Development of the Hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Belal Shohayeb,Uda Y Ho,Halah Hassan,Michael Piper,Dominic C H Ng

Cerebellum (London, England) 19:89-101 PubMed31838646

2019

Common Regulatory Targets of NFIA, NFIX and NFIB during Postnatal Cerebellar Development.

Applications

Unspecified application

Species

Unspecified reactive species

James Fraser,Alexandra Essebier,Alexander S Brown,Raul Ayala Davila,Danyon Harkins,Oressia Zalucki,Lauren P Shapiro,Peter Penzes,Brandon J Wainwright,Matthew P Scott,Richard M Gronostajski,Mikael Bodén,Michael Piper,Tracey J Harvey
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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