Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker - Alexa Fluor® 647 conjugated (ab196175) is a rabbit recombinant monoclonal antibody detecting S100 beta in ICC/IF. Suitable for Human.
- Biophysical QC for unrivalled batch-batch consistency
Images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB196175), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-s100-beta-antibody-ep1576y-astrocyte-marker-ab196175--immunocytochemistry-immunofluorescence-img173248.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB196175)")
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB196175), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-s100-beta-antibody-ep1576y-astrocyte-marker-ab196175--immunocytochemistry-immunofluorescence-img69676.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB196175)")
Publications
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- JACC. Clinical electrophysiology 8:1191-12152022The Heart's Pacemaker Mimics Brain Cytoarchitecture and Function: Novel Interstitial Cells Expose Complexity of the SAN.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRostislav Bychkov,Magdalena Juhaszova,Miguel Calvo-Rubio Barrera,Lorenzo A H Donald,Christopher Coletta,Chad Shumaker,Kayla Moorman,Syevda Tagirova Sirenko,Alexander V Maltsev,Steven J Sollott,Edward G LakattaPubMed 36182566
- STAR protocols 3:1011092022A clearing-free protocol for imaging intact whole adipose tissue innervation in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJake W Willows,Magdalena Blaszkiewicz,Kristy L TownsendPubMed 35106499
- iScience 24:1031272021Visualization and analysis of whole depot adipose tissue neural innervation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJake W Willows,Magdalena Blaszkiewicz,Amy Lamore,Samuel Borer,Amanda L Dubois,Emma Garner,William P Breeding,Karissa B Tilbury,Andre Khalil,Kristy L TownsendPubMed 34622172
- Scientific reports 11:161752021Increased mortality and altered local immune response in secondary peritonitis after previous visceral operations in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJonas Menz,Laura Hundt,Tobias Schulze,Katrin Schmoeckel,Pia Menges,Grazyna DomanskaPubMed 34376743
- Frontiers in cell and developmental biology 8:5493532020The Spindle-Associated Microcephaly Protein, WDR62, Is Required for Neurogenesis and Development of the Hippocampus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBelal Shohayeb et. al.PubMed 33042990
- Cerebellum (London, England) 19:89-1012019Common Regulatory Targets of NFIA, NFIX and NFIB during Postnatal Cerebellar Development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames Fraser,Alexandra Essebier,Alexander S Brown,Raul Ayala Davila,Danyon Harkins,Oressia Zalucki,Lauren P Shapiro,Peter Penzes,Brandon J Wainwright,Matthew P Scott,Richard M Gronostajski,Mikael Bodén,Michael Piper,Tracey J HarveyPubMed 31838646
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Goat | Predicted |
| Macaque monkey | Predicted |
| Zebrafish | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes This product gave a positive signal in A375 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Goat, Zebrafish, Macaque monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
3 products for Alternative Version
Target data
Function
Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain (PubMed:20950652, PubMed:6487634). Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer (PubMed:20950652, PubMed:6487634). Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites (By similarity). Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation (By similarity). Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue (By similarity). Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase (By similarity). Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization (PubMed:20351179). May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (PubMed:22399290).
Alternative names
Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B
Recommended products
Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker - Alexa Fluor® 647 conjugated (ab196175) is a rabbit recombinant monoclonal antibody detecting S100 beta in ICC/IF. Suitable for Human.
- Biophysical QC for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP1576Y
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 5.5 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab196175) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
Related products
Antibody clone EP1576Y is also available pre-conjugated to a variety of labels for your convenience – Anti-S100 beta Alexa Fluor® 647 [EP1576Y] - Astrocyte Marker (ab196175).
Other related products
We have a range of other formats of antibody clone [EP1576Y] also available for your convenience:
Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker ab52642, HRP - HRP Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker ab195535, Alexa Fluor® 647 - ab196175, Carrier free - Anti-S100 beta antibody [EP1576Y] - BSA and Azide free ab215989, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker ab307851, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker ab310990, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker ab313219, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker ab313817
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
S100 beta also known as protein S100 is part of the S100 family of proteins characterized by their calcium-binding properties. The S100 beta protein has an approximate molecular mass of 10.7 kDa. This protein is mainly expressed in astrocytes a type of glial cell in the central nervous system. It acts as a marker for astrocytes and is prominently used in astrocyte immunohistochemistry. Additionally it serves as a valuable marker for identifying and studying astrocyte markers because it can highlight the presence of these cells in various contexts.
- Biological function summary
The S100 beta protein participates in several intracellular and extracellular activities influencing cell proliferation and differentiation. It forms dimers and sometimes assembles into larger complexes mediated by the binding of calcium ions which alters its functional state. Within the nervous system S100 beta modulates the dynamics between neurons and glial cells affecting processes such as cell survival and neurite outgrowth. This dual role in both supporting and inhibiting various cellular actions highlights its importance in maintaining neural homeostasis.
- Pathways
S100 beta engages in signaling pathways related to inflammation and stress responses. It works within the MAPK and NF-kB pathways playing roles in cellular responses to oxidative stress. These pathways involve interactions with other proteins like p53 which S100 beta can modulate to influence apoptosis. The regulatory properties of S100 beta in these pathways link it with broader networks affecting cell fate decisions under pathological and physiological conditions.
- Associated diseases and disorders
S100 beta is associated with neurodegenerative diseases and cancer. Elevated levels of S100 beta are often found in Alzheimer's disease where it interacts with proteins like amyloid-beta contributing to neuroinflammation and neurotoxicity. In cancer particularly malignant melanoma S100 beta levels serve as a biomarker for tumor progression. Its interactions with p53 and other regulatory proteins highlight its involvement in oncogenic processes emphasizing its potential as a target for therapeutic intervention in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab196175), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-s100-beta-antibody-ep1576y-astrocyte-marker-ab196175--immunocytochemistry-immunofluorescence-img173248.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab196175)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab196175)
ab196175 staining S100 beta in Malme-3M cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196175 at 1/100 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab196175), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-s100-beta-antibody-ep1576y-astrocyte-marker-ab196175--immunocytochemistry-immunofluorescence-img69676.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab196175)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab196175)
ab196175 staining S100 beta in A375 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196175 at 1/50 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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