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AB325113

Alexa Fluor® 647 Anti-SAP102 antibody [EPR28704-52]

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Rabbit Recombinant Monoclonal SAP102 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-Fr and reacts with Mouse samples.

View Alternative Names

Dlgh3, Dlg3, Disks large homolog 3, Synapse-associated protein 102, SAP-102, SAP102

2 Images
Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-SAP102 antibody [EPR28704-52] (AB325113)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-SAP102 antibody [EPR28704-52] (AB325113)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh frozen) tissue labeling SAP102 with ab325113 at 1/50 (10.0 ug/ml) dilution (White).

Panel A : merged staining of anti-DLG3 (ab325113, white), anti-NeuN (ab190195, green) and anti-GFAP (ab201732, magenta) on mouse hippocampus.
Panel B : anti-DLG3 stained on mouse hippocampus.
Panel C : anti-NeuN stained in neurons of mouse hippocampus.
Panel D : anti-GFAP stained in astrocytes of mouse hippocampus.
The section was incubated in two rounds of staining : in the order of ab325113 and ab190195, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-SAP102 antibody [EPR28704-52] (AB325113)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-SAP102 antibody [EPR28704-52] (AB325113)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (fresh frozen) tissue labeling SAP102 with ab325113 at 1/50 (10.0 ug/ml) dilution (Magenta).

Negative control : confocal image showing no staining on mouse skeletal muscle (PMID : 8780649). The nuclear counterstain was DAPI (Blue). The section was incubated with ab325113 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28704-52

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Mouse

Applications

IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Unsuitable for human IHC, human ICC and human FC.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SAP102 also known as DLG3 is a member of the membrane-associated guanylate kinase (MAGUK) protein family. This protein weighs approximately 97 kDa and is mainly expressed in the brain. SAP102 plays a significant role in signal transduction by organizing synaptic signaling complexes. It contains PDZ domains that facilitate protein-protein interactions and its expression in neuronal synapses is notable.
Biological function summary

The interaction between SAP102 and N-methyl-D-aspartate (NMDA) receptors has major implications in synapse development and plasticity. SAP102 forms part of the postsynaptic density (PSD) complex which contributes to synaptic organization and dynamics. This complex is essential for the correct functioning of synaptic signaling pathways influencing learning and memory processes.

Pathways

The regulation of synaptic plasticity and neurotransmitter release involves SAP102. In this context it plays a part in the NMDA receptor signaling pathway and the synaptic vesicle cycle. SAP102 is associated with proteins such as PSD95 and SAP97 which work together to modulate synaptic strength and communication.

Abnormalities in SAP102 function or expression have links to neurodevelopmental disorders like intellectual disability and autism spectrum disorder. Mutations in SAP102 and related proteins such as neuroligins or neurexins can disrupt synaptic communication leading to defective neural circuit formation. Understanding SAP102's role in these disorders offers insights into potential therapeutic approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for learning most likely through its role in synaptic plasticity following NMDA receptor signaling.
See full target information Dlg3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com